LOCUS       sequence-25586-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pCAG-EGFP/RFP-miR-E1-1int
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           325..339
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 acacatcgtg gacagtcgag cgcaccgagg gccgccacca cctgttcctg taattaatta
       61 acaggtaagt gcgatcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca
      121 ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt
      181 tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg
      241 cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggtac ctgctgttga cagtgagcga
      301 atgagaagat ctcttgcaca tcaagtgaag ccacagatgt tgatgtgcaa gatcttctca
      361 tctgcctact gcctcggact tcaaggggaa ttcagggtgg ggccacctgc cggtaggtgt
      421 gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg ctctcctgaa
      481 tcgacaggcg ccggacctct ggcggccgca acaacgcgtt cctgaccatt catcctcttt
      541 ctttttcctg caggcttgtg gaagaaatgg gaccctgctg ctgctggaga cggccactgc
      601 tccctgagtg tcccgtccct ggggctcctg cttccccncn cgaaaattcc cctgctccna
      661 cctcctgtcg nnctgcctgc tcaccgatct ctcccgatcc taanaagntt tgcggnantn
      721 nngncatgaa ccttccctcc ccgtgcgctc tgggactgag ccatganaca anctgccctt
      781 ggtgcnnnga ntcnaaccca cccaggantg
//
