LOCUS       sequence-255870-        9808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes FKBP-dCas9(N) fused to VEGFR2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Baeumler TA, Ahmed AA, Fulga TA
  TITLE     Engineering Synthetic Signaling Pathways with Programmable 
            dCas9-Based Chimeric Receptors.
  JOURNAL   Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 
            10.1016/j.celrep.2017.08.044.
  PUBMED    28903044
REFERENCE   2  (bases 1 to 9808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-12- 12"; volume: "20"; issue: "11"; pages: "2639-2653"
COMMENT     Sequence Label: 
            pVEGFR2_TEV(N)_NES_TCS(Q'L)_FKBP_HA-dCas9(N)_P2A_Puro-WPRE
FEATURES             Location/Qualifiers
     source          1..9808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          117..344
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             3182..3502
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             3509..3535
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(3551..3574,3575..3577,3578..5152)
                     /codon_start=1
                     /product="N-terminal portion of catalytically inactive 
                     Streptococcus pyogenes dCas9 carrying the D10A mutation 
                     (Zetsche et al., 2015)"
                     /label=dCas9(N)
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMR"
     CDS             5183..5239
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(5246..5265)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5742..5762
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5879..5899)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6297..6308)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6439..6456)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6445..6652
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6661..6801
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6661..6790
                     /label=AAV2 ITR
     rep_origin      6876..7331
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6963..6982)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7173..7194
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7348..7367)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7467..7489
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7527..7545)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7613..7717
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7718..7786,7787..8578)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7936..7955)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8749..9337
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9238..9257
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        9408..9693
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
       61 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      121 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      181 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      241 gctgtaatta gctgagcaag aggtaagggt ttaagggatg gttggttggt ggggtattaa
      301 tgtttaatta cctggagcac ctgcctgaaa tcactttttt tcaggttgga ccggtgccac
      361 catgcagagc aaggtgctgc tggccgtcgc cctgtggctc tgcgtggaga cccgggccgc
      421 ctctgtgggt ttgcctagtg tttctcttga tctgcccagg ctcagcatac aaaaagacat
      481 acttacaatt aaggctaata caactcttca aattacttgc aggggacaga gggacttgga
      541 ctggctttgg cccaataatc agagtggcag tgagcaaagg gtggaggtga ctgagtgcag
      601 cgatggcctc ttctgtaaga cactcacaat tccaaaagtg atcggaaatg acactggagc
      661 ctacaagtgc ttctaccggg aaactgactt ggcctcggtc atttatgtct atgttcaaga
      721 ttacagatct ccatttattg cttctgttag tgaccaacat ggagtcgtgt acattactga
      781 gaacaaaaac aaaactgtgg tgattccatg tctcgggtcc atttcaaatc tcaacgtgtc
      841 actttgtgca agatacccag aaaagagatt tgttcctgat ggtaacagaa tttcctggga
      901 cagcaagaag ggctttacta ttcccagcta catgatcagc tatgctggca tggtcttctg
      961 tgaagcaaaa attaatgatg aaagttacca gtctattatg tacatagttg tcgttgtagg
     1021 gtataggatt tatgatgtgg ttctgagtcc gtctcatgga attgaactat ctgttggaga
     1081 aaagcttgtc ttaaattgta cagcaagaac tgaactaaat gtggggattg acttcaactg
     1141 ggaataccct tcttcgaagc atcagcataa gaaacttgta aaccgagacc taaaaaccca
     1201 gtctgggagt gagatgaaga aatttttgag caccttaact atagatggta taacccggag
     1261 tgaccaagga ttgtacacct gtgcagcatc cagtgggctg atgaccaaga agaacagcac
     1321 atttgtcagg gtccatgaaa aaccttttgt tgcttttgga agtggcatgg aatctctggt
     1381 ggaagccacg gtgggggagc gtgtcagaat ccctgcgaag taccttggtt acccaccccc
     1441 agaaataaaa tggtataaaa atggaatacc ccttgagtcc aatcacacaa ttaaagcggg
     1501 gcatgtactg acgattatgg aagtgagtga aagagacaca ggaaattaca ctgtcatcct
     1561 taccaatccc atttcaaagg agaagcagag ccatgtggtc tctctggttg tgtatgtccc
     1621 accccagatt ggtgagaaat ctctaatctc tcctgtggat tcctaccagt acggcaccac
     1681 tcaaacgctg acatgtacgg tctatgccat tcctcccccg catcacatcc actggtattg
     1741 gcagttggag gaagagtgcg ccaacgagcc cagccaagct gtctcagtga caaacccata
     1801 cccttgtgaa gaatggagaa gtgtggagga cttccaggga ggaaataaaa ttgaagttaa
     1861 taaaaatcaa tttgctctaa ttgaaggaaa aaacaaaact gtaagtaccc ttgttatcca
     1921 agcggcaaat gtgtcagctt tgtacaaatg tgaagcggtc aacaaagtcg ggagaggaga
     1981 gagggtgatc tccttccacg tgaccagggg tcctgaaatt actttgcaac ctgacatgca
     2041 gcccactgag caggagagcg tgtctttgtg gtgcactgca gacagatcta cgtttgagaa
     2101 cctcacatgg tacaagcttg gcccacagcc tctgccaatc catgtgggag agttgcccac
     2161 acctgtttgc aagaacttgg atactctttg gaaattgaat gccaccatgt tctctaatag
     2221 cacaaatgac attttgatca tggagcttaa gaatgcatcc ttgcaggacc aaggagacta
     2281 tgtctgcctt gctcaagaca ggaagaccaa gaaaagacat tgcgtggtca ggcagctcac
     2341 agtcctagag cgtgtggcac ccacgatcac aggaaacctg gagaatcaga cgacaagtat
     2401 tggggaaagc atcgaagtct catgcacggc atctgggaat ccccctccac agatcatgtg
     2461 gtttaaagat aatgagaccc ttgtagaaga ctcaggcatt gtattgaagg atgggaaccg
     2521 gaacctcact atccgcagag tgaggaagga ggacgaaggc ctctacacct gccaggcatg
     2581 cagtgttctt ggctgtgcaa aagtggaggc atttttcata atagaaggtg cccaggaaaa
     2641 gacgaacttg gaaatcatta ttctagtagg cacggcggtg attgccatgt tcttctggct
     2701 acttcttgtc atcatcggcg gtggctcggg tggtggctcg ggagaaagct tgtttaaggg
     2761 gccgcgtgat tacaacccga tatcgagcac catttgtcat ttgacgaatg aatctgatgg
     2821 gcacacaaca tcgttgtatg gtattggatt tggtcccttc atcattacaa acaagcactt
     2881 gtttagaaga aataatggaa cactgttggt ccaatcacta catggtgtat tcaaggtcaa
     2941 gaacaccacg actttgcaac aacacctcat tgatgggagg gacatgataa ttattcgcat
     3001 gcctaaggat ttcccaccat ttcctcaaaa gctgaaattt agagagccac aaagggaaga
     3061 gcgcatatgt cttgtgacaa ccaacttcca aactggcggt ggctcgctag atttagctag
     3121 cttaatcctc ggcaagcttg gcgaaaacct gtattttcag ctaggcggag gcagcactag
     3181 tggcgtgcag gtggagacca tcagccccgg cgacggcaga accttcccca agagaggcca
     3241 gacctgcgtg gtgcactaca ccggcatgct ggaggacggc aagaagttcg acagcagcag
     3301 agacagaaac aagcccttca agttcatgct gggcaagcag gaggtgatca gaggctggga
     3361 ggagggcgtg gcccagatga gcgtgggcca gagagccaag ctgaccatca gccccgacta
     3421 cgcctacggc gccaccggcc accccggcat catccctcct cacgccaccc tggtgttcga
     3481 cgtggagctg ctgaagctgg agactagtta cccatacgac gtaccagatt acgctggagg
     3541 ttctggatcc gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg
     3601 ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac
     3661 cgaccggcac agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac
     3721 agccgaggcc acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg
     3781 gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt
     3841 ccacagactg gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat
     3901 cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct
     3961 gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct
     4021 ggcccacatg atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa
     4081 cagcgacgtg gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga
     4141 aaaccccatc aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa
     4201 gagcagacgg ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt
     4261 cggcaacctg attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct
     4321 ggccgaggat gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct
     4381 gctggcccag atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga
     4441 cgccatcctg ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag
     4501 cgcctctatg atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct
     4561 cgtgcggcag cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg
     4621 ctacgccggc tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc
     4681 catcctggaa aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct
     4741 gctgcggaag cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga
     4801 gctgcacgcc attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga
     4861 aaagatcgag aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg
     4921 aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt
     4981 cgaggaagtg gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt
     5041 cgataagaac ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt
     5101 caccgtgtat aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaggtggcgg
     5161 tggctcgggt accggatctg gagccaccaa tttttctctg ctgaagcagg ctggtgatgt
     5221 tgaagaaaac cctggacctg aattcatgac cgagtacaag cccacggtgc gcctcgccac
     5281 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     5341 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     5401 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     5461 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     5521 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     5581 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     5641 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     5701 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     5761 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     5821 catgacccgc aaggggtaac taagtaagga tcgatccaag atatcgtatt cttaactatg
     5881 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     5941 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6001 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6061 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6121 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6181 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     6241 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6301 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6361 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgccaattc
     6421 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     6481 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     6541 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     6601 ggcaggacag caagggggag gattgggaag agaatagcag gcatgctggg gagcggccgc
     6661 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
     6721 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
     6781 gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg
     6841 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
     6901 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc
     6961 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     7021 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     7081 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     7141 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     7201 actcaactct atctcgggct attcttttga tttataaggg attttgccga tttcggtcta
     7261 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     7321 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     7381 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     7441 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     7501 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     7561 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     7621 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7681 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7741 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7801 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7861 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7921 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     7981 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     8041 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     8101 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     8161 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     8221 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     8281 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     8341 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8401 tattgctgat aaatctggag ccggtgagcg tggaagccgc ggtatcattg cagcactggg
     8461 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     8521 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8581 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8641 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8701 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8761 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8821 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8881 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8941 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     9001 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     9061 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     9121 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     9181 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9241 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9301 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9361 acggttcctg gccttttgct ggccttttgc tcacatctag aggtacccgt tacataactt
     9421 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaatagta
     9481 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     9541 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     9601 aaatggcccg cctggcattg tgcccagtac atgaccttat gggactttcc tacttggcag
     9661 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
     9721 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
     9781 ttttgtgcag cgatgggggc gggggggg
//
