LOCUS       sequence-255877-         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-133a-5p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-133a-5p
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(101..125)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(349..368)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    414..469
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(468..487)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 gccgacaaag agacatacgt cgagcagcac gaggtggctg tggccagata ctgcgacctc
       61 cctagcaaac tggggcacaa acttaattga gaattctacc acccagcttt cttgtacaaa
      121 gtggtacgtc tcaagaccag aggtctctgc ttcacggtcg atttggttcc attttaccag
      181 ctatttggtt ccattttacc agctatttgg ttccatttta ccagctattt ggttccattt
      241 taccagctca accagaagag acctgtgctt cactcctcag gtgcaggctg cctatcagaa
      301 ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc
      361 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
      421 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
      481 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
      541 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat
      601 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gc
//
