LOCUS       sequence-255895-        7087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid contains a reporter gene for Crz1. The 1000bp promoter of 
            the gene pYPS1 driving a Venus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Chen SY, Osimiri LC, Chevalier M, Bugaj LJ, Nguyen TH, Greenstein 
            RA, Ng AH, Stewart-Ornstein J, Neves LT, El-Samad H
  TITLE     Optogenetic Control Reveals Differential Promoter Interpretation of 
            Transcription Factor Nuclear Translocation Dynamics.
  JOURNAL   Cell Syst. 2020 Sep 4. pii: S2405-4712(20)30293-3. doi: 
            10.1016/j.cels.2020.08.009.
  PUBMED    32898473
REFERENCE   2  (bases 1 to 7087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2020 Sep 4. pii: S2405-4712(20)30293-3. doi: 
            10.1016/j.cels.2020.08.009."
COMMENT     Sequence Label: pYPS1-Venus
FEATURES             Location/Qualifiers
     source          1..7087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2561..3376)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2561..3376)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3284..3303
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3368..3387
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      3601..4189
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4090..4109
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        4539..4946
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             6130..6843
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEGGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      6859..7046
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6914..6938)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gaaatctgct cgtcagtggt gctcacactg acgaatcatg tacagatcat accgatgact
       61 gcctggcgac tcacaactaa gcaagacagc cggaaccagc gccggcgaac accactgcat
      121 atatggcata tcacaacagt ccacgtctca agcagttaca gagatgttac gaaccactag
      181 tgcactgcag tacattacca atagatagcg gtttaatata aggtttcaac aagaaatatt
      241 ttgtgaaaat caaaagttaa tagtttcact gtcgaaaata tgaaagttta agttttcagt
      301 tcatcccatt gtagttatca aatttctgtg tttcccgtat atgtgtgctc tgtattcatc
      361 gctatcttga aactggttat gatcgttgcg catgtgacct gtatcgctca aaagagcccg
      421 gcaccggtac cgggcaaaag cccctagcaa gctaaaatga ttcaactttc gtagtaattt
      481 gttcaaccgg tcacagtaaa gtcaatgact aacttttgaa tcgaggatgt atttccaatt
      541 gaaagcaatg tatatataaa ccattgtgtt gaagcttgtc ttacagtttt cttgtatcct
      601 cccattgttc atatagtact taaagtacca taatccttaa agaacaaact tgaaagagaa
      661 tacactatac acaactaata aataaagaat aatcatggct gtgaccaaga caattgtagt
      721 tctaccaggt gaccatgttg gtcaagaaat cactgaagag gccattaaag ttttaaatgc
      781 tattcaggaa tgtcgtccag acaaggtcaa tttcaagttt gatcatcatt tgatcggtgg
      841 tgctgcaatt gatgccactg gtgttccatt accagacgaa gctttggagg cctctaagaa
      901 agctgatgcc gtgcttctgg gtgctgttgg tggtccaaaa tggggtactg gcgctgtcag
      961 accagaacaa ggtcttttga aaatccgtaa ggagttgcaa ttgtatgcta atctaagacc
     1021 atgtaatttt gcatctgatt ccttactaga tctatcgcca ttgaagcctg aaattgcaag
     1081 aggtacagat ttcgtcgttg ttagagaact agtgggtggt atttatttcg gggagagaaa
     1141 agaagatgaa ggtgatggtg tcgcctggga tagcgaaaag tattctgtgc ccgaagttca
     1201 aagaatcact agaatggctg ctttcatggc tctgcaacac aacccacctt tacctatttg
     1261 gactctagat aaagccaacg ttctagcatc atcaagatta tggagaaaaa cagtggaaaa
     1321 gacgattaag aatgaattcc cacaattgac tgttcaacat caattgattg attccgctgc
     1381 tatgatcttg gttaagaacc caactcatct aaatggtatt attatcacaa acaatatgtt
     1441 tggtgatatt atctccgatg aagcctccgt gatcccaggt tcgttaggat tacttccatc
     1501 tgcctccttg gcatctcttc cagataagaa tacggcattt ggtttatatg agccatgtca
     1561 tggctctgct ccagacttac caaagggaaa ggttaacccc gtagcaacaa tcctatctgc
     1621 tgctatgatg ttgaagttgt ctctggacct gttcgaagaa ggtgaaatta tagaacaagc
     1681 agtcaagaaa gtgttggatt ctggtatcag aacagcggat ctaaagggta ccaactctac
     1741 tactgaggtc ggtgatgcag tagcgaaagc tgtcagggaa ctattagctt agacttagta
     1801 gatgtacagt tgcatacata agtctgaaca gaaagaagta aatatcatat ctagttcggt
     1861 ttagaaatca gtgactatat tggttttgac cagatcgcaa tccttgcaag catttagtaa
     1921 tgactatcaa accggtgaat catctgataa ggctatatta gcttgtgcat tcgcatgtat
     1981 cgggaaacga acgagtccac cgaagtcggt gatgctgtcg ccgaagaagt taagaaaatc
     2041 cttgcttaaa aagattctct ttttttatga tatttgtaca taaactttat aaatgaaatt
     2101 cataatagaa acgacacgaa attacaaaat ggaatatgtt catagggtag acgaaactat
     2161 atacgcaatc tacatacatt tatcaagaag gagaaaaagg aggatgtaaa ggaatacagg
     2221 taagcaaatt gatactaatg gctcaacgtg ataaggaaaa agaattgcac tttaacatta
     2281 atattgacaa ggaggagggc accacacaaa aagttaggtg taacagaaaa tcatgaaact
     2341 atgattccta atttatatat tggaggattt tctctaaaaa aaaaaaaata caacaaataa
     2401 aaaacactca atgacctgac catttgatgg agtttaagtc aataccttct tgaaccattt
     2461 cccataatgg tgaaagttcc ctcaagaatt ttactctgtc agaaacggcc ttaacgacgt
     2521 accgagcggc cgcgtgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca
     2581 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     2641 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     2701 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     2761 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     2821 aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     2881 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg aggcgaaata
     2941 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     3001 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     3061 ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     3121 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     3181 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     3241 tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     3301 acccatataa atcagcatcc atgttggaat ttaatcgcgg cctggagcaa gacgtttccc
     3361 gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg
     3421 ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaacgt
     3481 ggctttgttg aataaatcga acttttgctg agttgaagga tcagtcatga ccaaaatccc
     3541 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3601 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3661 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3721 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3781 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3841 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3901 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3961 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4021 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4081 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4141 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4201 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4261 gttatcccct gattctgtgg ataaccgtgc ggccgccaat gaggagaact tctagtatat
     4321 ctacatacct aatattattg ccttattaaa aatggaatcc caacaattac atcaaaatcc
     4381 acattctctt caaaatcaat tgtcctgtac ttccttgttc atgtgtgttc aaaaacgtta
     4441 tatttatagg ataattatac tctatttctc aacaagtaat tggttgtttg gccgagcggt
     4501 ctaaggcgcc tgattcaaga aatatcttga ccgcagttaa ctgtgggaat actcaggtat
     4561 cgtaagatgc aagagttcga atctcttagc aaccattatt tttttcctca acataacgag
     4621 aacacacagg ggcgctatcg cacagaatca aattcgatga ctggaaattt tttgttaatt
     4681 tcagaggtcg cctgacgcat ataccttttt caactgaaaa attgggagaa aaaggaaagg
     4741 tgagagcgcc ggaaccggct tttcatatag aatagagaag cgttcatgac taaatgcttg
     4801 catcacaata cttgaagttg acaatattat ttaaggacct attgtttttt ccaataggtg
     4861 gttagcaatc gtcttacttt ctaacttttc ttacctttta catttcagca atatatatat
     4921 atatatttca aggatatacc attctaccct gaattcgcat ctagactgat gagacgtggt
     4981 agagccacaa acagccggta caagcaacga tctccaggac catctgaatc atgcgcggat
     5041 gacacgaact cacgacggcg atcacagaca ttaacccaca gtacagacac tgcgacaacg
     5101 tggcaattcg tcgcaataca acgcagacca gtgtggggac ctgtgtcttg gcaataattc
     5161 cacaagcggg caacgccacc gctatccttg gtgattcctt cttgagaaac gcctacgtgg
     5221 tctacgattt ggataactac gagatttccc tagctcaagc caagtatggc acggggaaag
     5281 agaacgtcga agtcatcaaa tctaccgttc ccagtgcaat aagggccccc agttacaaca
     5341 acacttggtc taactacgcc tccgccacgt ccggtggtaa tatttttacc accgtgcgca
     5401 ctttcaatgg caccagtact gccaccacta cgaggtcaac caccaccaag aagacaaact
     5461 ctaccactac tgcaaagtcg actcataaaa gcaagagggc actccagagg gctgctacca
     5521 actccgcttc cagtatacgc tctaccttgg gtttactgct agtcccctcc ttactcatcc
     5581 tttccgtttt cttttcgtaa aaaaaaacaa tagacactat atatagacac tttttccttt
     5641 ccttctttgc gcgatttcaa gaggaaaagc atacttaaat aagaatattc ctaaaacaca
     5701 cgttctgacg cgtcaattag atcgtcagac cttgcatgca gccaagacac cctcacatag
     5761 cactgcctcc ttcctcctct tttctgtcac cacctcacct ccctcgtcca ctcaactgag
     5821 tggcttttcg ctccttttat actgcgccat gagtagtttt cgtttcactg atgtgtccga
     5881 aaaaattgag gtttcataaa aaaattcgtg gacttattta tggagaaaca gggaaatccg
     5941 actacttaag aaaagggtgt caaagaggat ttactttttt ccttcttttt gcatttgttc
     6001 ctatttccgc aattggacgg ttattaagaa gaacgcaatt ggcttttctg tatattaaaa
     6061 tacatagcgt aataaaaaga taaggtgaac accaagcata tagtataata ttacctacca
     6121 catagatcta tgtctaaagg tgaagaatta ttcactggtg ttgtcccaat tttggttgaa
     6181 ttagatggtg atgttaatgg tcacaaattt tctgtctccg gtgaaggtga aggtgatgct
     6241 acttacggta aattgacctt aaaattgatt tgtactactg gtaaattgcc agttccatgg
     6301 ccaaccttag tcactacttt aggttatggt ttgcaatgtt ttgctagata cccagatcat
     6361 atgaaacaac atgacttttt caagtctgcc atgccagaag gttatgttca agaaagaact
     6421 atttttttca aagatgacgg taactacaag accagagctg aagtcaagtt tgaaggtgat
     6481 accttagtta atagaatcga attaaaaggt attgatttta aagaaggtgg taacatttta
     6541 ggtcacaaat tggaatacaa ctataactct cacaatgttt acatcactgc tgacaaacaa
     6601 aagaatggta tcaaagctaa cttcaaaatt agacacaaca ttgaagatgg tggtgttcaa
     6661 ttagctgacc attatcaaca aaatactcca attggtgatg gtccagtctt gttaccagac
     6721 aaccattact tatcctatca atctgcctta tccaaagatc caaacgaaaa gagagatcac
     6781 atggtcttgt tagaatttgt tactgctgct ggtattaccc atggtatgga tgaattgtac
     6841 aaaggatcct aactcgaggc gaatttctta tgatttatga tttttattat taaataagtt
     6901 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc
     6961 ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc
     7021 gctcttattg accacacctc taccggcatg ccgagcaaat gcctgcaaat cgctccccat
     7081 ttcgctg
//
