LOCUS       sequence-255908-       11688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TPI-PTC1 NMD reporter (It has a Premature termination 
            codon (PTC) at First Amino acid).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11688)
  AUTHORS   Hoek TA, Khuperkar D, Lindeboom RGH, Sonneveld S, Verhagen BMP, 
            Boersma S, Vermeulen M, Tanenbaum ME
  TITLE     Single-Molecule Imaging Uncovers Rules Governing Nonsense-Mediated 
            mRNA Decay.
  JOURNAL   Mol Cell. 2019 May 23. pii: S1097-2765(19)30361-2. doi: 
            10.1016/j.molcel.2019.05.008.
  PUBMED    31155380
REFERENCE   2  (bases 1 to 11688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 May 23. pii: S1097-2765(19)30361-2. doi: 
            10.1016/j.molcel.2019.05.008."
COMMENT     Sequence Label: TPI-PTC1
FEATURES             Location/Qualifiers
     source          1..11688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      984..993
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1059..1115
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1131..1187
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1203..1259
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1275..1331
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1347..1403
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1419..1475
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1491..1547
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1563..1619
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1635..1691
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1707..1763
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1779..1835
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1851..1907
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1923..1979
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1995..2051
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2067..2123
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2139..2195
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2211..2267
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2283..2339
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2355..2411
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2427..2483
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2499..2555
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2571..2627
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2715..2771
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     complement(7685..7702)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7691..7915
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7961..8389
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8048..8067)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8258..8279
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8398..8418)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8403..8732
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8583..8718
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8645..8664
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8780..8827
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8846..9220
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9350..9471
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9387..9406)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9441..9460
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9520..9536)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9520..9536)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9533..9555)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9544..9560
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9568..9574,9575..9592,9593..9598))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9613..9634
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9751..9768)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9933..10521)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10013..10032)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10692..11483,11484..11552))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11315..11334
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11553..11657)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaaactaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttgccacca tgggcgccat catacacgcg gccgcaatca
     1021 tcggtccggg tggatctgga ggtggaggtt ctggaggaga agaacttttg agcaagaatt
     1081 atcatcttga gaacgaagtg gctcgtctta agaaaggttc tggcagtgga gaagaactgc
     1141 tttcaaagaa ttaccacctg gaaaatgagg tagctagact gaaaaagggg agcggaagtg
     1201 gggaggagtt gctgagcaaa aattatcatt tggagaacga agtagcacga ctaaagaaag
     1261 ggtccggatc gggtgaggag ttactctcga aaaattatca tctcgaaaac gaagtggctc
     1321 ggctaaaaaa gggcagtggt tctggagaag agctattatc taaaaactac cacctcgaaa
     1381 atgaggtggc acgcttaaaa aagggaagtg gcagtggtga agagctacta tccaagaatt
     1441 atcatcttga gaacgaggta gcgcgtttga agaagggttc cggctcagga gaggaactgc
     1501 tctcgaagaa ctatcatctt gaaaatgagg tcgctcgatt aaaaaaggga tcgggcagtg
     1561 gtgaggaact actttcaaag aattaccacc tcgaaaacga agtagctcga ttaaagaaag
     1621 gttcagggtc gggtgaagaa ttactgagta aaaattatca tctggaaaat gaggtagcga
     1681 gactaaaaaa ggggagtggt tctggcgaag agttgctatc gaaaaattat catcttgaga
     1741 acgaagttgc taggctcaaa aagggctcag gctcaggcga ggagttgctc tcgaaaaact
     1801 accacttgga aaatgaggtc gcgaggttga aaaaggggag cgggtcgggc gaggagttat
     1861 tgagcaaaaa ctatcattta gagaacgaag tcgcgcgctt aaagaaaggc tcgggctcgg
     1921 gcgaagaact cttatcgaag aactaccacc tcgaaaatga ggtcgccagg ttgaaaaagg
     1981 gcagtggcag cggggaggaa ctcttgagca agaactacca cttggagaat gaggtcgcga
     2041 gattgaagaa agggtcgggg agcggcgagg aattgctcag caagaattat catttggaga
     2101 acgaagtcgc caggctcaag aaaggctcgg ggtcggggga ggaattgttg agtaaaaact
     2161 accacttgga aaatgaagtc gccaggctca aaaaagggag tgggagcggc gaagagttat
     2221 tgagcaaaaa ttaccacttg gagaacgaag tggcaaggct caagaaaggg agcggcagcg
     2281 gggaggagct cttatcgaag aactaccact tagagaatga agtcgcccgc ttgaagaaag
     2341 gctcggggag cggggaagag ctcttgagca agaactacca cttggaaaat gaggtggcgc
     2401 gcttgaagaa agggagcggg agcggggaag agttactatc taagaattat catctcgaga
     2461 acgaggtggc tcgactaaag aagggctccg gcagtgggga ggaactcctg tcgaagaact
     2521 atcatcttga aaatgaggtt gcaagactta aaaaggggtc cggatcaggt gaggaactac
     2581 tcagtaagaa ttaccacctg gaaaacgaag ttgcacgttt gaagaaagga tcaggatcag
     2641 gcgaagaact gctctcaaaa gattatcatt tggaaaatga ggttgcacgt ttaaaaaagg
     2701 gaagtggcag tggtgaggaa cttctgtcga aaaattatca tctcgagaat gaagtagccc
     2761 gacttaaaaa gggttctggc tcgggtcagc ggccgcaccg gtcatctgag ctgattaaag
     2821 aaaatatgca tatgaagctg tatatggagg ggacagtgga caatcaccat ttcaaatgta
     2881 catctgaagg agaggggaaa ccctatgaag gaacacagac aatgaggatc aaagtggtgg
     2941 aggggggccc cctgcctttc gcttttgaca ttctggctac cagctttctg tatggaagca
     3001 agactttcat caaccatacc cagggaattc ctgatttctt taaacagtcc ttccctgagg
     3061 gatttacatg ggaacgggtg acaacttacg aggacggcgg ggtgctgacc gccactcagg
     3121 atacaagcct gcaggacggc tgcctgatct ataatgtgaa gatcagaggc gtgaacttca
     3181 cttccaatgg ccccgtgatg cagaagaaga ccctgggctg ggaggcattc actgagactc
     3241 tgtacccagc tgacggggga ctggaagggc gcaacgatat ggcactgaaa ctggtggggg
     3301 gaagccatct gattgcaaac attaaaacta cataccgctc taaaaaaccc gcaaaaaacc
     3361 tgaaaatgcc aggcgtgtat tacgtggatt accgcctgga gcgcatcaaa gaggctaata
     3421 acgagaccta cgtggaacag cacgaggtgg ctgtggctcg ctactgcgat ctgccttcta
     3481 agctgggaca caaactgaac gatatctaac cctccaggaa gttcttcgtt gggggaaact
     3541 ggaagatgaa cgggcggaag cagagtctgg gggagctcat cggcactctg aacgcggcca
     3601 aggtgccggc cgacaccggt aagccctcgc cgaggagggg tctggccggg ccggggccgg
     3661 ggccggggca ggagtggcag cgccctctcc cgaggccccg aggccccgag gccggtatcc
     3721 gcgcggacct gatgcagggc tgtgggacga gggccgctgg ggtccgggca ggggcctcgc
     3781 agccgcagcc ccgtcggtgc gtcgaggggg cagggcggag cacatgatgc cccttggact
     3841 atggggcagg taaggacgtt ttcttccctt cggcaacctg aacgactccc gccttccacg
     3901 gaagggaccg agcccgtgcc aaacaggctg agcgatttgg gagtgaggag ccatcctacc
     3961 gctttcccca acctggaaac agcaaagcgc aaggcctctg agtcagttag gtctctgcca
     4021 cccacgggca aaggatgctc tcctccatcc tccttcctcc ctccaccgaa atcggagagc
     4081 cgcgggcctg atccaaagag gcatcccctt ctcgttcatt ccccagaggc ctcaatacaa
     4141 accccaggag ttggcccctc tccttttgct acaaatcctt gccttgcaaa ggggaggtga
     4201 ggatgggcta ttttagaagg gaagcagggt tgctccctgg agaatgctga gtctgtgagg
     4261 tgcctatgcc gagaataggt cgaggaaatt ggagccccag ctgttaaaag agcagagggc
     4321 agggtgaggg ccgtggcctc tcaggggtat ctggaaggct cttcgagttg agtgcagacc
     4381 cagcctgggc tggaaaatgg acaaaggtca tcttgctggg gtgaaaaggg ggagagcaga
     4441 accaagaaga agagggtgag ggctgggggg ctccagggca ctggttagga attgtgggga
     4501 atgaaggctt tctttagtct catccccctg tggtaccatc ttgtcctcag aggtggtttg
     4561 tgctccccct actgcctata tcgacttcgc ccggcagaag ctagatccca agattgctgt
     4621 ggctgcgcag aactgctaca aagtgactaa tggggctttt actggggaga tcaggtgaga
     4681 tcgaggtgga gaggggtgtg tgggaccctt ccctcacttt cctcgttgag gggaaagcca
     4741 cagggtgggc tccctgctga accttggctt catctcttcc tttagccctg gcatgatcaa
     4801 agactgcgga gccacgtggg tggtcctggg gcactcagag agaaggcatg tctttgggga
     4861 gtcagatgag gttagtagcc aagagagaag ataagggatg tctttttcca agaaggatgt
     4921 ctcaccaagt ctgtttctca acagctgatt gggcagaaag tggcccatgc tctggcagag
     4981 ggactcggag taatcgcctg cattggggag aagctagatg aaagggaagc tggcatcact
     5041 gagaaggttg ttttcgagca gacaaaggtc atcgcaggta tctctggaga aagggacctt
     5101 tgagcctatc cagggccaca gagactcaga gggtagggtc aggccctgga gcctgtcttg
     5161 gtccccatgc tgatccagaa aaggaaaaag gggaggggga gtgacaatct ttgcttgggg
     5221 cctatgactt ctccagcccc aaggtagatg ccacctggaa atcccccaat gtccactagg
     5281 gggcagtagg ccaccgttct tcgtactccg gagaacctgg ctggagagct ctttcttgtt
     5341 cacccttccc tccatctgta tctctgccct gcagataacg tgaaggactg gagcaaggtc
     5401 gtcctggcct atgagcctgt gtgggccatt ggtactggca agactgcaac accccaacag
     5461 gtaaccgggc ccaggagccc tgccctcatc ccagcctgcc tcaataggtt tggacagaca
     5521 cagcccacat ggggcaaccc cttatttcaa agacacagag accttgaacc cagagacagt
     5581 gacttgtcca agggcatcca gtccagggcc tggcttggat cagagccctg gtactctgac
     5641 tcagtcagaa accacactaa gtgtccactg gtgccagtga tttttcctct tagagaggca
     5701 gaaaaggtct tacttaggcc agcttcttgt tctaggccca ggaagtacac gagaagctcc
     5761 gaggatggct gaagtccaac gtctctgatg cggtggctca gagcacccgt atcatttatg
     5821 gaggtgagtg gctttggttc ccggctgagg tggagtgggc tgaggactag actgagccct
     5881 cggacatgga ggtggggatg gggcagactc atcccattct tgaccaagcc cttgttctgc
     5941 tcccttccca ggctctgtga ctggggcaac ctgcaaggag ctggccagcc agcctgatgt
     6001 ggatggcttc cttgtgggtg gtgcttccct caagcccgaa ttcgtggaca tcatcaatgc
     6061 caaacaatga gccccatcca tcttccctac ccttcctgcc aagccaggga ctgctagccc
     6121 ggttgtacag gtaccggatc ctaaggtacc taattgccta gaaaggagca gacgatatgg
     6181 cgtcgctccc tgcaggtcga ctctagaaac cagcagagca tatgggctcg ctggctgcag
     6241 tattcccggg ttcattagat cctaaggtac ctaattgcct agaaaggagc agacgatatg
     6301 gcgtcgctcc ctgcaggtcg actctagaaa ccagcagagc atatgggctc gctggctgca
     6361 gtattcccgg gttcattaga tcctaaggta cctaattgcc tagaaaggag cagacgatat
     6421 ggcgtcgctc cctgcaggtc gactctagaa accagcagag catatgggct cgctggctgc
     6481 agtattcccg ggttcattag atcctaaggt acctaattgc ctagaaagga gcagacgata
     6541 tggcgtcgct ccctgcaggt cgactctaga aaccagcaga gcatatgggc tcgctggctg
     6601 cagtattccc gggttcatta gatcctaagg tacctaattg cctagaaagg agcagacgat
     6661 atggcgtcgc tccctgcagg tcgactctag aaaccagcag agcatatggg ctcgctggct
     6721 gcagtattcc cgggttcatt agatcctaag gtacctaatt gcctagaaag gagcagacga
     6781 tatggcgtcg ctccctgcag gtcgactcta gaaaccagca gagcatatgg gctcgctggc
     6841 tgcagtattc ccgggttcat tagatcctaa ggtacctaat tgcctagaaa ggagcagacg
     6901 atatggcgtc gctccctgca ggtcgactct agaaaccagc agagcatatg ggctcgctgg
     6961 ctgcagtatt cccgggttca ttagatccta aggtacctaa ttgcctagaa aggagcagac
     7021 gatatggcgt cgctccctgc aggtcgactc tagaaaccag cagagcatat gggctcgctg
     7081 gctgcagtat tcccgggttc attagatcct aaggtaccta attgcctaga aaggagcaga
     7141 cgatatggcg tcgctccctg caggtcgact ctagaaacca gcagagcata tgggctcgct
     7201 ggctgcagta ttcccgggtt cattagatcc taaggtacct aattgcctag aaaggagcag
     7261 acgatatggc gtcgctccct gcaggtcgac tctagaaacc agcagagcat atgggctcgc
     7321 tggctgcagt attcccgggt tcattagatc ctaaggtacc taattgccta gaaaggagca
     7381 gacgatatgg cgtcgctccc tgcaggtcga ctctagaaac cagcagagca tatgggctcg
     7441 ctggctgcag tattcccggg ttcattagat cctaaggtac ctaattgcct agaaaggagc
     7501 agacgatatg gcgtcgctcc ctgcaggtcg actctagaaa ccagcagagc atatgggctc
     7561 gctggctgca gtattcccgg gttcattaga tctcgcgaag ggcgaattca cgaggcgcgc
     7621 ccggatcgcg atcgcaatct taattaactc gagtctagag ggcccgttta aacccgctga
     7681 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     7741 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     7801 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     7861 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     7921 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     7981 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     8041 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     8101 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     8161 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     8221 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     8281 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     8341 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     8401 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     8461 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     8521 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     8581 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     8641 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     8701 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     8761 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     8821 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
     8881 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
     8941 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
     9001 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
     9061 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
     9121 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
     9181 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
     9241 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     9301 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     9361 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     9421 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9481 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     9541 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     9601 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     9661 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     9721 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     9781 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     9841 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca gcaaaaggcc
     9901 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     9961 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    10021 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    10081 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    10141 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    10201 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    10261 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    10321 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    10381 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    10441 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    10501 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    10561 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    10621 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    10681 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    10741 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    10801 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    10861 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    10921 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    10981 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    11041 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    11101 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    11161 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    11221 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    11281 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    11341 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    11401 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    11461 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    11521 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    11581 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    11641 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
