LOCUS       sequence-25591-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pCAG-RFP-miR-E1-1int
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           324..338
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 acatcgtgna cagtacgagc gcaccgaggg ccgccaccac ctgttcctgt aattaattaa
       61 caggtaagtg cnatcgctaa tgcgggaaag ctcttattcg ggtgagatgg gctggggcac
      121 catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt gtcgtctgtt
      181 gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc
      241 gccctcgtcg tgtcgtgacg tcacccgttc tgttggtacc tgctgttgac agtgagcgaa
      301 tgagaagatc tcttgcacat caagtgaagc cacagatgtt gatgtgcaag atcttctcat
      361 ctgcctactg cctcggactt caaggggaat tcagggtggg gccacctgcc ggtaggtgtg
      421 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      481 cgacaggcgc cggacctctg gcggccgcaa caacgcgttc ctgaccattc atcctctttc
      541 tttttcctgc aggcttgtgg aagaaatggg accctgctgc tgctggagac ncannaacna
      601 nanngnnncc cngncccggg gttnncctgt tncccatccc ggantctcct gctcccactc
      661 tgngctgctg ctcagccaaa acnatcnnnt cccggantcc tananagagn cctgccatga
      721 actnccctcc cggcctnggg gaaagacnca tgaacaactg ccntggtgtn cgatcctacc
      781 acccagaatg agtggctngg nctncccaaa anggggcggc accccccnan ccaggngnaa
      841 tatnatc
//
