LOCUS       sequence-255912-       10629 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Beta-globin-PTC39 NMD reporter (It has a Premature 
            termination codon (PTC) at 39th Amino acid).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10629)
  AUTHORS   Hoek TA, Khuperkar D, Lindeboom RGH, Sonneveld S, Verhagen BMP, 
            Boersma S, Vermeulen M, Tanenbaum ME
  TITLE     Single-Molecule Imaging Uncovers Rules Governing Nonsense-Mediated 
            mRNA Decay.
  JOURNAL   Mol Cell. 2019 May 23. pii: S1097-2765(19)30361-2. doi: 
            10.1016/j.molcel.2019.05.008.
  PUBMED    31155380
REFERENCE   2  (bases 1 to 10629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 May 23. pii: S1097-2765(19)30361-2. doi: 
            10.1016/j.molcel.2019.05.008."
COMMENT     Sequence Label: Beta-Globin-PTC39
FEATURES             Location/Qualifiers
     source          1..10629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      984..993
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1059..1115
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1131..1187
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1203..1259
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1275..1331
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1347..1403
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1419..1475
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1491..1547
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1563..1619
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1635..1691
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1707..1763
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1779..1835
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1851..1907
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1923..1979
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1995..2051
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2067..2123
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2139..2195
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2211..2267
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2283..2339
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2355..2411
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2427..2483
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2499..2555
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2571..2627
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2715..2771
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     complement(6637..6654)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6643..6867
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6913..7341
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7000..7019)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7210..7231
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7350..7370)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7355..7684
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7535..7670
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7597..7616
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7732..7779
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7798..8172
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8302..8423
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8339..8358)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8393..8412
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8472..8488)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8472..8488)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8485..8507)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8496..8512
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8520..8526,8527..8544,8545..8550))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8565..8586
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8703..8720)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8874..9462)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8954..8973)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9633..10424,10425..10493))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10256..10275
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10494..10598)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaaactaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttgccacca tgggcgccat catacacgcg gccgcaatca
     1021 tcggtccggg tggatctgga ggtggaggtt ctggaggaga agaacttttg agcaagaatt
     1081 atcatcttga gaacgaagtg gctcgtctta agaaaggttc tggcagtgga gaagaactgc
     1141 tttcaaagaa ttaccacctg gaaaatgagg tagctagact gaaaaagggg agcggaagtg
     1201 gggaggagtt gctgagcaaa aattatcatt tggagaacga agtagcacga ctaaagaaag
     1261 ggtccggatc gggtgaggag ttactctcga aaaattatca tctcgaaaac gaagtggctc
     1321 ggctaaaaaa gggcagtggt tctggagaag agctattatc taaaaactac cacctcgaaa
     1381 atgaggtggc acgcttaaaa aagggaagtg gcagtggtga agagctacta tccaagaatt
     1441 atcatcttga gaacgaggta gcgcgtttga agaagggttc cggctcagga gaggaactgc
     1501 tctcgaagaa ctatcatctt gaaaatgagg tcgctcgatt aaaaaaggga tcgggcagtg
     1561 gtgaggaact actttcaaag aattaccacc tcgaaaacga agtagctcga ttaaagaaag
     1621 gttcagggtc gggtgaagaa ttactgagta aaaattatca tctggaaaat gaggtagcga
     1681 gactaaaaaa ggggagtggt tctggcgaag agttgctatc gaaaaattat catcttgaga
     1741 acgaagttgc taggctcaaa aagggctcag gctcaggcga ggagttgctc tcgaaaaact
     1801 accacttgga aaatgaggtc gcgaggttga aaaaggggag cgggtcgggc gaggagttat
     1861 tgagcaaaaa ctatcattta gagaacgaag tcgcgcgctt aaagaaaggc tcgggctcgg
     1921 gcgaagaact cttatcgaag aactaccacc tcgaaaatga ggtcgccagg ttgaaaaagg
     1981 gcagtggcag cggggaggaa ctcttgagca agaactacca cttggagaat gaggtcgcga
     2041 gattgaagaa agggtcgggg agcggcgagg aattgctcag caagaattat catttggaga
     2101 acgaagtcgc caggctcaag aaaggctcgg ggtcggggga ggaattgttg agtaaaaact
     2161 accacttgga aaatgaagtc gccaggctca aaaaagggag tgggagcggc gaagagttat
     2221 tgagcaaaaa ttaccacttg gagaacgaag tggcaaggct caagaaaggg agcggcagcg
     2281 gggaggagct cttatcgaag aactaccact tagagaatga agtcgcccgc ttgaagaaag
     2341 gctcggggag cggggaagag ctcttgagca agaactacca cttggaaaat gaggtggcgc
     2401 gcttgaagaa agggagcggg agcggggaag agttactatc taagaattat catctcgaga
     2461 acgaggtggc tcgactaaag aagggctccg gcagtgggga ggaactcctg tcgaagaact
     2521 atcatcttga aaatgaggtt gcaagactta aaaaggggtc cggatcaggt gaggaactac
     2581 tcagtaagaa ttaccacctg gaaaacgaag ttgcacgttt gaagaaagga tcaggatcag
     2641 gcgaagaact gctctcaaaa gattatcatt tggaaaatga ggttgcacgt ttaaaaaagg
     2701 gaagtggcag tggtgaggaa cttctgtcga aaaattatca tctcgagaat gaagtagccc
     2761 gacttaaaaa gggttctggc tcgggtcagc ggccgcaccg gtcatctgag ctgattaaag
     2821 aaaatatgca tatgaagctg tatatggagg ggacagtgga caatcaccat ttcaaatgta
     2881 catctgaagg agaggggaaa ccctatgaag gaacacagac aatgaggatc aaagtggtgg
     2941 aggggggccc cctgcctttc gcttttgaca ttctggctac cagctttctg tatggaagca
     3001 agactttcat caaccatacc cagggaattc ctgatttctt taaacagtcc ttccctgagg
     3061 gatttacatg ggaacgggtg acaacttacg aggacggcgg ggtgctgacc gccactcagg
     3121 atacaagcct gcaggacggc tgcctgatct ataatgtgaa gatcagaggc gtgaacttca
     3181 cttccaatgg ccccgtgatg cagaagaaga ccctgggctg ggaggcattc actgagactc
     3241 tgtacccagc tgacggggga ctggaagggc gcaacgatat ggcactgaaa ctggtggggg
     3301 gaagccatct gattgcaaac attaaaacta cataccgctc taaaaaaccc gcaaaaaacc
     3361 tgaaaatgcc aggcgtgtat tacgtggatt accgcctgga gcgcatcaaa gaggctaata
     3421 acgagaccta cgtggaacag cacgaggtgg ctgtggctcg ctactgcgat ctgccttcta
     3481 agctgggaca caaactgaac gatatcatgg tgcatctgac tcctgaggag aagtctgccg
     3541 ttactgccct gtggggcaag gtgaacgtgg atgaagttgg tggtgaggcc ctgggcaggt
     3601 tggtatcaag gttacaagac aggtttaagg agaccaatag aaactgggca tgtggagaca
     3661 gagaagactc ttgggtttct gataggcact gactctctct gcctattggt ctattttccc
     3721 acccttaggc tgctggtggt ctacccttgg acctagaggt tctttgagtc ctttggggat
     3781 ctgtccactc ctgatgctgt tatgggcaac cctaaggtga aggctcatgg caagaaagtg
     3841 ctcggtgcct ttagtgatgg cctggctcac ctggacaacc tcaagggcac ctttgccaca
     3901 ctgagtgagc tgcactgtga caagctgcac gtggatcctg agaacttcag ggtgagtcta
     3961 tgggaccctt gatgttttct ttccccttct tttctatggt taagttcatg tcataggaag
     4021 gggataagta acagggtaca gtttagaatg ggaaacagac gaatgattgc atcagtgtgg
     4081 aagtctcagg atcgttttag tttcttttat ttgctgttca taacaattgt tttcttttgt
     4141 ttaattcttg ctttcttttt ttttcttctc cgcaattttt actattatac ttaatgcctt
     4201 aacattgtgt ataacaaaag gaaatatctc tgagatacat taagtaactt aaaaaaaaac
     4261 tttacacagt ctgcctagta cattactatt tggaatatat gtgtgcttat ttgcatattc
     4321 ataatctccc tactttattt tcttttattt ttaattgata cataatcatt atacatattt
     4381 atgggttaaa gtgtaatgtt ttaatatgtg tacacatatt gaccaaatca gggtaatttt
     4441 gcatttgtaa ttttaaaaaa tgctttcttc ttttaatata cttttttgtt tatcttattt
     4501 ctaatacttt ccctaatctc tttctttcag ggcaataatg atacaatgta tcatgcctct
     4561 ttgcaccatt ctaaagaata acagtgataa tttctgggtt aaggcaatag caatatttct
     4621 gcatataaat atttctgcat ataaattgta actgatgtaa gaggtttcat attgctaata
     4681 gcagctacaa tccagctacc attctgcttt tattttatgg ttgggataag gctggattat
     4741 tctgagtcca agctaggccc ttttgctaat catgttcata cctcttatct tcctcccaca
     4801 gctcctgggc aacgtgctgg tctgtgtgct ggcccatcac tttggcaaag aattcacccc
     4861 accagtgcag gctgcctatc agaaagtggt ggctggtgtg gctaatgccc tggcccacaa
     4921 gtatcactaa gctcgctttc ttgctgtcca atttctatta aaggttcctt tgttccctaa
     4981 gtccaactac taaactgggg gatattatga agggccttga gcatctggat tctgcctaat
     5041 aaaaaacatt tattttcatt gcaagctagc ccggttgtac aggtaccgga tcctaaggta
     5101 cctaattgcc tagaaaggag cagacgatat ggcgtcgctc cctgcaggtc gactctagaa
     5161 accagcagag catatgggct cgctggctgc agtattcccg ggttcattag atcctaaggt
     5221 acctaattgc ctagaaagga gcagacgata tggcgtcgct ccctgcaggt cgactctaga
     5281 aaccagcaga gcatatgggc tcgctggctg cagtattccc gggttcatta gatcctaagg
     5341 tacctaattg cctagaaagg agcagacgat atggcgtcgc tccctgcagg tcgactctag
     5401 aaaccagcag agcatatggg ctcgctggct gcagtattcc cgggttcatt agatcctaag
     5461 gtacctaatt gcctagaaag gagcagacga tatggcgtcg ctccctgcag gtcgactcta
     5521 gaaaccagca gagcatatgg gctcgctggc tgcagtattc ccgggttcat tagatcctaa
     5581 ggtacctaat tgcctagaaa ggagcagacg atatggcgtc gctccctgca ggtcgactct
     5641 agaaaccagc agagcatatg ggctcgctgg ctgcagtatt cccgggttca ttagatccta
     5701 aggtacctaa ttgcctagaa aggagcagac gatatggcgt cgctccctgc aggtcgactc
     5761 tagaaaccag cagagcatat gggctcgctg gctgcagtat tcccgggttc attagatcct
     5821 aaggtaccta attgcctaga aaggagcaga cgatatggcg tcgctccctg caggtcgact
     5881 ctagaaacca gcagagcata tgggctcgct ggctgcagta ttcccgggtt cattagatcc
     5941 taaggtacct aattgcctag aaaggagcag acgatatggc gtcgctccct gcaggtcgac
     6001 tctagaaacc agcagagcat atgggctcgc tggctgcagt attcccgggt tcattagatc
     6061 ctaaggtacc taattgccta gaaaggagca gacgatatgg cgtcgctccc tgcaggtcga
     6121 ctctagaaac cagcagagca tatgggctcg ctggctgcag tattcccggg ttcattagat
     6181 cctaaggtac ctaattgcct agaaaggagc agacgatatg gcgtcgctcc ctgcaggtcg
     6241 actctagaaa ccagcagagc atatgggctc gctggctgca gtattcccgg gttcattaga
     6301 tcctaaggta cctaattgcc tagaaaggag cagacgatat ggcgtcgctc cctgcaggtc
     6361 gactctagaa accagcagag catatgggct cgctggctgc agtattcccg ggttcattag
     6421 atcctaaggt acctaattgc ctagaaagga gcagacgata tggcgtcgct ccctgcaggt
     6481 cgactctaga aaccagcaga gcatatgggc tcgctggctg cagtattccc gggttcatta
     6541 gatctcgcga agggcgaatt cacgaggcgc gcccggatcg cgatcgcaat cttaattaac
     6601 tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     6661 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     6721 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     6781 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     6841 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     6901 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6961 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     7021 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     7081 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     7141 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     7201 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     7261 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     7321 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     7381 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     7441 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     7501 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     7561 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7621 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     7681 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     7741 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     7801 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
     7861 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
     7921 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
     7981 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
     8041 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
     8101 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
     8161 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     8221 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     8281 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     8341 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     8401 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     8461 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     8521 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     8581 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     8641 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8701 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8761 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8821 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8881 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8941 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9001 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     9061 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9121 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9181 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9241 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9301 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9361 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9421 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9481 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9541 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9601 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9661 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9721 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9781 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9841 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9901 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9961 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    10021 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    10081 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    10141 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    10201 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    10261 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    10321 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    10381 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10441 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    10501 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10561 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10621 cctgacgtc
//
