LOCUS       sequence-255913-       12621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of plant nuclear envelope LINC protein fused to 
            fluorescent protein and affinity tags for cell biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12621)
  AUTHORS   Gumber HK, McKenna JF, Tolmie AF, Jalovec AM, Kartick AC, Graumann 
            K, Bass HW
  TITLE     MLKS2 is an ARM domain and F-actin-associated KASH protein that 
            functions in stomatal complex development and meiotic chromosome 
            segregation.
  JOURNAL   Nucleus. 2019 Dec;10(1):144-166. doi: 10.1080/19491034.2019.1629795.
  PUBMED    31221013
REFERENCE   2  (bases 1 to 12621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/19491034.2019"; journalName: "Nucleus"; date: "2019-12"; 
            volume: "10"; issue: "1"; pages: "144-166"
COMMENT     Sequence Label: MLKS2del4exp
FEATURES             Location/Qualifiers
     source          1..12621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        650..994
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     938..958
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1094..1118
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1121..1123,1124..1126,1127..1837)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1847..1870
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1874..1900
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(3813..3837)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4209..4388
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             4424..5449
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      5647..5899
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5992..6016
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6537..7328
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7574..8162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8063..8082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8316..8333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8348..8488
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8414..8433)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8574..8596
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8832..9026
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9092..10165)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10594..11223)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12554..12578
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ccaagctcaa gctaagcttg agctctccca tatggtcgac tagagccaag ctgatctcct
       61 ttgccccgga gatcaccatg gacgactttc tctatctcta cgatctagga agaaagttcg
      121 acggagaagg tgacgatacc atgttcacca ccgataatga gaagattagc ctcttcaatt
      181 tcagaaagaa tgctgaccca cagatggtta gagaggccta cgcggcaggt ctcatcaaga
      241 cgatctaccc gagtaataat ctccaggaga tcaaatacct tcccaagaag gttaaagatg
      301 cagtcaaaag attcaggact aactgcatca agaacacaga gaaagatata tttctcaaga
      361 tcagaagtac tattccagta tggacgattc aaggcttgct tcataaacca aggcaagtaa
      421 tagagattgg agtctctaag aaagtagttc ctactgaatc aaaggccatg gagtcaaaaa
      481 ttcagatcga ggatctaaca gaactcgccg tgaagactgg cgaacagttc atacagagtc
      541 ttttacgact caatgacaag aagaaaatct tcgtcaacat ggtggagcac gacactctcg
      601 tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc
      661 aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca
      721 tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa
      781 aggctatcgt tcaagatgcc cctgccgaca gtggtcccaa agatggaccc ccacccacga
      841 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
      901 atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct
      961 ctatataagg aagttcattt catttggaga ggactccggt atttttacaa caattaccac
     1021 aacaaaacaa acaacaaaca acattacaat ttactattct agtcgacctg caggcggccg
     1081 cactagtgat atcacaagtt tgtacaaaaa agcaggcttc atggtctcca agggggagga
     1141 gctgttcacc ggggtggtcc ccatcctggt ggagctggat ggcgacgtta acgggcacaa
     1201 gttctcggtg tctggcgagg gggagggcga cgctacctac gggaagctca cgctgaagtt
     1261 catctgcacg accggcaagc tgccggtgcc ctggccaaca ctcgtcacaa ctctgactta
     1321 cggcgtccag tgcttctcgc gctaccctga tcacatgaag cagcatgact tcttcaagtc
     1381 ggctatgccc gagggctacg tgcaggagcg gacgatcttc ttcaaggacg atggcaacta
     1441 caagacacgc gcggaggtca agttcgaggg ggacactctc gttaacagga tcgagctgaa
     1501 gggcattgac ttcaaggagg atggcaatat cctcgggcac aagctggagt acaactacaa
     1561 ttcccataac gtgtacatca tggccgacaa gcagaagaac ggcattaagg tcaatttcaa
     1621 gatccggcac aacattgagg atggcagcgt tcagctcgcg gaccattacc agcagaacac
     1681 accgatcggg gatggccctg tcctcctgcc cgacaatcac tacctctcca ctcagagcgc
     1741 cctgtcgaag gacccgaacg agaagaggga ccatatggtg ctcctggagt tcgtcacagc
     1801 cgcgggcatc actctcggga tggatgagct gtacaagggg gcgggcgact acaaggacga
     1861 tgacgataag gcttacccct acgacgtgcc ggattacgcc ggggctggca tggggcgctc
     1921 actctccccg ctcctgaggc aggagctgga caacctggat aaggacgccg attctcgcag
     1981 ggctgccatg aaggctctca agtcatacgc ccgccacctg gatagcaagt cgatccccca
     2041 tttcctggcc gaggtctctg acacgaccgc cgctggcgcg ggggccggca gcgggatgcc
     2101 aaccggcggg gagttcacga tctcgctcta cgaggtcctg gccagggttc acgggcggaa
     2161 catcgtgccc cagatcggca atattatggc gaccattatg tgcacgctct ccagctcggg
     2221 cgggtcattc ccactgcacc aggcctgctc caaggttgtg ccagctatcg ctaggcatgg
     2281 cattgatccg tcggccccag acggcgagaa ggccgggatc attgctagcc tctgccgccc
     2341 actgtgctcg gctctcatgg gcgggagcca ggacggcggc gctgccagcg gcgctgccct
     2401 ctgcctgaag gccctggttg agtcttcaaa ctggaggttc gcgtcgggcg agctggtgaa
     2461 tgaggtctgc ctgaaggtgg ccggcgctat gcacgactcg gccacacggt cgaacgctca
     2521 tatggggctg gccatggcgc tcgtgaagca caatggcctc atcgctgagg cctacgcgcg
     2581 ctctatcgtc aggtccggcc tccagattct ggacggggat accgctgact ccagctcgca
     2641 gaagaggctg tccgccatcc agatgattaa cttcttcatg aagttcgtgg acccgaggtg
     2701 cctgtcttca gagctgggcc gggttatcga tgtgatggag cgctgccaga atgaccggat
     2761 gcccttcgtg cggggcgcgg ctttcgaggc ctctcagtca gcgcgcaaca ttgccgcgca
     2821 gaagggctcc aggcacgagg ttgggacctc tcctatggtg ggctcaaact tccataagag
     2881 gagggagaag tccccttgcc ggagcctctg gggcgccaag aggtcccccg ctgcctctac
     2941 gatggccgct gctagccctg tgcagttccg ctctccggag tcacaggtcg ttgattccag
     3001 catcatgaat ggctccacac tccacgactc cccagtgagc gtcggccagt cgtcttgcaa
     3061 cttcgaccag agcaggcgga ctaaccgcag gctgtggtcg aatgacgcgg ttgatgtgtc
     3121 tctgaaggac ggcctcttca tccagctgtg ctccaacagc aattcgtacg aggacgatct
     3181 gggcgaggtt tgcgatagcg aggtgaccga cgctaacttc gagtgcacca atacgttcgc
     3241 cgggttcgtc tctccctcac acaatggctc catcagcagg gataagacgc cttcaccgcg
     3301 ggcctcggac aggcccatca acattgacga tgtgaagatc tactccacgc caaggaagct
     3361 cctgcggctc ctgcagtcat cctacgactc ggattctgcc tcaaacatcg ggcagagcac
     3421 ggccaagctc aggggcctct cctcccccga ccaggagcac aagggcctcg tcatttcttc
     3481 agagcagatg cagtcgctgc attctgacaa taagggcgat gagatgaagg acgagaacga
     3541 gaccatctac atgcagaata gcaagggcag gacacagact ctctcgaacg ctgacgcctc
     3601 agtcctgtcc acaactgagg ccgagaacac aagcatcaag acttcgcctg agattgagct
     3661 gaatggggac gatgtttgca tcacctccag cattggcaag acgcggaagt accgcgcgaa
     3721 gttcaccctc ttcctgtccg tcatcgttat tttcctcgct atcattacga tgctgatccg
     3781 cattgagaac gacgatgaca gcgcgtactg agacccagct ttcttgtaca aagtggtgat
     3841 atcccgcggc catgctagag tccgcaaaaa tcaccagtct ctctctacaa atctatctct
     3901 ctctattttt ctccagaata atgtgtgagt agttcccaga taagggaatt agggttctta
     3961 tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa
     4021 aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtgac ctgcaggcat
     4081 gcgacgtcgg gccctctaga ggatccccgg gtaccgcgaa ttatcgatca tgagcggaga
     4141 attaagggag tcacgttatg acccccgccg atgacgcggg acaagccgtt ttacgtttgg
     4201 aactgacaga accgcaacgt tgaaggagcc actgagccgc gggtttctgg agtttaatga
     4261 gctaagcaca tacgtcagaa accattattg cgcgttcaaa agtcgcctaa ggtcactatc
     4321 agctagcaaa tatttcttgt caaaaatgct ccactgacgt tccataaatt cccctcggta
     4381 tccaattaga gtctcatatt cactctcaac tcgaatcccc cctatgaaaa agcctgaact
     4441 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
     4501 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
     4561 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
     4621 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggagt tcagcgagag
     4681 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
     4741 cgaactgccc gctgttcttc agccggtcgc ggaggctatg gatgctatcg ctgcggccga
     4801 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
     4861 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
     4921 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
     4981 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac
     5041 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
     5101 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac
     5161 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccaaggctcc gggcgtatat
     5221 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
     5281 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
     5341 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
     5401 cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataga gtagatgccg
     5461 accgaacaag agctgatttc gagaacgcct cagccagcaa ctcgcgcgag cctagcaagg
     5521 caaatgcgag agaacggcct tacgcttggt ggcacagttc tcgtccacag ttcgctaagc
     5581 tcgctcggct gggtcgcggg agggccggtc gcagtgattc aggaattaat tccctagagt
     5641 caagcagatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     5701 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     5761 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     5821 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     5881 tcatctatgt tactagatcg accggcatgc aagctgataa ttcaattcgg cgttaattca
     5941 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
     6001 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
     6061 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
     6121 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
     6181 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
     6241 gctgcctgtg atcaattcgg gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     6301 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     6361 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
     6421 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     6481 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg
     6541 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     6601 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     6661 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     6721 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     6781 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     6841 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     6901 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     6961 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     7021 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     7081 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     7141 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     7201 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     7261 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     7321 gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac
     7381 tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg
     7441 cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgca
     7501 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7561 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7621 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7681 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     7741 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7801 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7861 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7921 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7981 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8041 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8101 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8161 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8221 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8281 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8341 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     8401 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     8461 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     8521 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     8581 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat
     8641 gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg
     8701 gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg
     8761 ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct
     8821 ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt
     8881 tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt
     8941 tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg
     9001 ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta
     9061 gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga
     9121 tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat
     9181 cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga
     9241 actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct
     9301 tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa
     9361 agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca
     9421 tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct
     9481 tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct
     9541 tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt
     9601 cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg
     9661 gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg
     9721 ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg
     9781 gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc
     9841 gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg
     9901 tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct
     9961 tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag
    10021 cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg
    10081 cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg
    10141 ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt
    10201 gcagggccgg cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat
    10261 ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc
    10321 cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt
    10381 attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg
    10441 tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc
    10501 tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg
    10561 tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc
    10621 ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt
    10681 gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg
    10741 cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc
    10801 ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac
    10861 cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag
    10921 cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc
    10981 gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc
    11041 gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc
    11101 ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag
    11161 atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt
    11221 catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca
    11281 tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct
    11341 tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt
    11401 tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc
    11461 atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt
    11521 ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca
    11581 atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc
    11641 gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca
    11701 cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg
    11761 tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc
    11821 ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc
    11881 ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg
    11941 cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta
    12001 gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg
    12061 ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt
    12121 gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca
    12181 gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat
    12241 tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag
    12301 cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac
    12361 gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt taaagtactt
    12421 taaagtactt taaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa
    12481 atggacgaac ggataaacct tttcacgccc ttttaaatat ccgattattc taataaacgc
    12541 tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaactgaa
    12601 ggcgggaaac gacaatctga t
//
