LOCUS       sequence-255915-       12600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of plant nuclear envelope LINC protein fused to 
            fluorescent protein and affinity tags for cell biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12600)
  AUTHORS   Gumber HK, McKenna JF, Tolmie AF, Jalovec AM, Kartick AC, Graumann 
            K, Bass HW
  TITLE     MLKS2 is an ARM domain and F-actin-associated KASH protein that 
            functions in stomatal complex development and meiotic chromosome 
            segregation.
  JOURNAL   Nucleus. 2019 Dec;10(1):144-166. doi: 10.1080/19491034.2019.1629795.
  PUBMED    31221013
REFERENCE   2  (bases 1 to 12600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/19491034.2019"; journalName: "Nucleus"; date: "2019-12"; 
            volume: "10"; issue: "1"; pages: "144-166"
COMMENT     Sequence Label: MLKS2delKASHexp
FEATURES             Location/Qualifiers
     source          1..12600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    33..57
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        750..1094
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1038..1058
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1194..1218
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1221..1223,1224..1226,1227..1937)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1947..1970
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1974..2000
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(3892..3916)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4288..4467
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             4503..5528
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      5726..5978
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6071..6095
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6616..7407
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7653..8241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8142..8161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8395..8412
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8427..8567
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8493..8512)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8653..8675
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8911..9105
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9171..10244)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10673..11302)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac
       61 actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctaagcttg
      121 agctctccca tatggtcgac tagagccaag ctgatctcct ttgccccgga gatcaccatg
      181 gacgactttc tctatctcta cgatctagga agaaagttcg acggagaagg tgacgatacc
      241 atgttcacca ccgataatga gaagattagc ctcttcaatt tcagaaagaa tgctgaccca
      301 cagatggtta gagaggccta cgcggcaggt ctcatcaaga cgatctaccc gagtaataat
      361 ctccaggaga tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact
      421 aactgcatca agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta
      481 tggacgattc aaggcttgct tcataaacca aggcaagtaa tagagattgg agtctctaag
      541 aaagtagttc ctactgaatc aaaggccatg gagtcaaaaa ttcagatcga ggatctaaca
      601 gaactcgccg tgaagactgg cgaacagttc atacagagtc ttttacgact caatgacaag
      661 aagaaaatct tcgtcaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa
      721 gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga
      781 aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag
      841 gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc
      901 cctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
      961 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
     1021 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
     1081 catttggaga ggactccggt atttttacaa caattaccac aacaaaacaa acaacaaaca
     1141 acattacaat ttactattct agtcgacctg caggcggccg cactagtgat atcacaagtt
     1201 tgtacaaaaa agcaggcttc atggtctcca agggggagga gctgttcacc ggggtggtcc
     1261 ccatcctggt ggagctggat ggcgacgtta acgggcacaa gttctcggtg tctggcgagg
     1321 gggagggcga cgctacctac gggaagctca cgctgaagtt catctgcacg accggcaagc
     1381 tgccggtgcc ctggccaaca ctcgtcacaa ctctgactta cggcgtccag tgcttctcgc
     1441 gctaccctga tcacatgaag cagcatgact tcttcaagtc ggctatgccc gagggctacg
     1501 tgcaggagcg gacgatcttc ttcaaggacg atggcaacta caagacacgc gcggaggtca
     1561 agttcgaggg ggacactctc gttaacagga tcgagctgaa gggcattgac ttcaaggagg
     1621 atggcaatat cctcgggcac aagctggagt acaactacaa ttcccataac gtgtacatca
     1681 tggccgacaa gcagaagaac ggcattaagg tcaatttcaa gatccggcac aacattgagg
     1741 atggcagcgt tcagctcgcg gaccattacc agcagaacac accgatcggg gatggccctg
     1801 tcctcctgcc cgacaatcac tacctctcca ctcagagcgc cctgtcgaag gacccgaacg
     1861 agaagaggga ccatatggtg ctcctggagt tcgtcacagc cgcgggcatc actctcggga
     1921 tggatgagct gtacaagggg gcgggcgact acaaggacga tgacgataag gcttacccct
     1981 acgacgtgcc ggattacgcc ggggctggca tggggcgctc actctccccg ctcctgaggc
     2041 aggagctgga caacctggat aaggacgccg attctcgcag ggctgccatg aaggctctca
     2101 agtcatacgc ccgccacctg gatagcaagt cgatccccca tttcctggcc gaggtctctg
     2161 acacgaccgc cgctggcgcg ggggccggca gcgggatgcc aaccggcggg gagttcacga
     2221 tctcgctcta cgaggtcctg gccagggttc acgggcggaa catcgtgccc cagatcggca
     2281 atattatggc gaccattatg tgcacgctct ccagctcggg cgggtcattc ccactgcacc
     2341 aggcctgctc caaggttgtg ccagctatcg ctaggcatgg cattgatccg tcggccccag
     2401 acggcgagaa ggccgggatc attgctagcc tctgccgccc actgtgctcg gctctcatgg
     2461 gcgggagcca ggacggcggc gctgccagcg gcgctgccct ctgcctgaag gccctggttg
     2521 agtcttcaaa ctggaggttc gcgtcgggcg agctggtgaa tgaggtctgc ctgaaggtgg
     2581 ccggcgctat gcacgactcg gccacacggt cgaacgctca tatggggctg gccatggcgc
     2641 tcgtgaagca caatggcctc atcgctgagg cctacgcgcg ctctatcgtc aggtccggcc
     2701 tccagattct ggacggggat accgctgact ccagctcgca gaagaggctg tccgccatcc
     2761 agatgattaa cttcttcatg aagttcgtgg acccgaggtg cctgtcttca gagctgggcc
     2821 gggttatcga tgtgatggag cgctgccaga atgaccggat gcccttcgtg cggggcgcgg
     2881 ctttcgaggc ctctcagtca gcgcgcaaca ttgccgcgca gaagggctcc aggcacgagg
     2941 ttgggacctc tcctatggtg ggctcaaact tccataagag gagggagaag tccccttgcc
     3001 ggagcctctg gggcgccaag aggtcccccg ctgcctctac gatggccgct gctagccctg
     3061 tgcagttccg ctctccggag tcacaggtcg ttgattccag catcatgaat ggctccacac
     3121 tccacgactc cccagtgagc gtcggccagt cgtcttgcaa cttcgaccag agcaggcgga
     3181 ctaaccgcag gctgtggtcg aatgacgcgg ttgatgtgtc tctgaaggac ggcctcttca
     3241 tccagctgtg ctccaacagc aattcgtacg aggacgatct gggcgaggtt tgcgatagcg
     3301 aggtgaccga cgctaacttc gagtgcacca atacgttcgc cgggttcgtc tctccctcac
     3361 acaatggctc catcagcagg gataagacgc cttcaccgcg ggcctcggac aggcccatca
     3421 acattgacga tgtgaagatc tactccacgc caaggaagct cctgcggctc ctgcagtcat
     3481 cctacgactc ggattctgcc tcaaacatcg ggcagagcac ggccaagctc aggggcctct
     3541 cctcccccga ccaggagcac aagggcctcg tcatttcttc agagcagatg cagtcgctgc
     3601 attctgacaa taagggcgat gagatgaagg acgagaacga gaccatctac atgcagaata
     3661 gcaagggcag gacacagact ctctcgaacg ctgacgcctc agtcctgtcc acaactgagg
     3721 ccgagaacac aagcatcaag acttcgcctg agattgagct gaatggggac gatgtttgca
     3781 tcacctccag cattggcaag acgcggaagt accgcgcgaa gttcaccctc ttcctgtccg
     3841 tcatcgttat tttcctcgct atcattacga tgctgatcgc tgccgcatga gacccagctt
     3901 tcttgtacaa agtggtgata tcccgcggcc atgctagagt ccgcaaaaat caccagtctc
     3961 tctctacaaa tctatctctc tctatttttc tccagaataa tgtgtgagta gttcccagat
     4021 aagggaatta gggttcttat agggtttcgc tcatgtgttg agcatataag aaacccttag
     4081 tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa
     4141 tccagtgacc tgcaggcatg cgacgtcggg ccctctagag gatccccggg taccgcgaat
     4201 tatcgatcat gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga
     4261 caagccgttt tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctgagccgcg
     4321 ggtttctgga gtttaatgag ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa
     4381 gtcgcctaag gtcactatca gctagcaaat atttcttgtc aaaaatgctc cactgacgtt
     4441 ccataaattc ccctcggtat ccaattagag tctcatattc actctcaact cgaatccccc
     4501 ctatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg
     4561 acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg
     4621 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag
     4681 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca
     4741 ttggggagtt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt
     4801 tgcaagacct gcctgaaacc gaactgcccg ctgttcttca gccggtcgcg gaggctatgg
     4861 atgctatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag
     4921 gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt
     4981 atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg
     5041 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg
     5101 gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg
     5161 cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg
     5221 cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc
     5281 caaggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg
     5341 acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg
     5401 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg
     5461 gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa
     5521 aggaatagag tagatgccga ccgaacaaga gctgatttcg agaacgcctc agccagcaac
     5581 tcgcgcgagc ctagcaaggc aaatgcgaga gaacggcctt acgcttggtg gcacagttct
     5641 cgtccacagt tcgctaagct cgctcggctg ggtcgcggga gggccggtcg cagtgattca
     5701 ggaattaatt ccctagagtc aagcagatcg ttcaaacatt tggcaataaa gtttcttaag
     5761 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     5821 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     5881 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     5941 taaattatcg cgcgcggtgt catctatgtt actagatcga ccggcatgca agctgataat
     6001 tcaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
     6061 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc
     6121 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca
     6181 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa
     6241 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt
     6301 gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca gtggacataa
     6361 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     6421 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     6481 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     6541 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     6601 acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag
     6661 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     6721 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     6781 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     6841 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     6901 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     6961 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     7021 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     7081 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     7141 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     7201 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     7261 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     7321 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     7381 gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg
     7441 cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca
     7501 aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa
     7561 ttgggagata tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7621 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7681 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7741 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     7801 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7861 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7921 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7981 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     8041 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     8101 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     8161 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     8221 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     8281 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     8341 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     8401 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     8461 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     8521 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     8581 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     8641 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     8701 cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg
     8761 cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata
     8821 ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc
     8881 agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt
     8941 ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc
     9001 acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg
     9061 ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt
     9121 cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg
     9181 gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc
     9241 cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg
     9301 gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac
     9361 aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg
     9421 atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct
     9481 tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg
     9541 gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc
     9601 aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga
     9661 atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg
     9721 agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg
     9781 tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac
     9841 acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag
     9901 cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg
     9961 atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc
    10021 tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat
    10081 tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg
    10141 atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc
    10201 gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct
    10261 tgggggttcc agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac
    10321 cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt
    10381 ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac
    10441 tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc
    10501 gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg
    10561 gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg
    10621 ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga
    10681 gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt
    10741 ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga
    10801 tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga
    10861 tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga
    10921 cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc
    10981 ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg
    11041 ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc
    11101 ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg
    11161 cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga
    11221 gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt
    11281 taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat
    11341 catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag
    11401 acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc
    11461 agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa
    11521 cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc
    11581 ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc
    11641 agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg
    11701 ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc
    11761 agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg
    11821 atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc
    11881 tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg
    11941 aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg
    12001 ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg
    12061 ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca
    12121 agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc
    12181 ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg
    12241 ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc
    12301 cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag
    12361 gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca
    12421 gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc
    12481 aaagtacttt aaagtacttt aaagtacttt aaagtacttt gatcccgagg ggaaccctgt
    12541 ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc
//
