LOCUS       sequence-255916-       12534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of plant nuclear envelope LINC protein fused to 
            fluorescent protein and affinity tags for cell biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12534)
  AUTHORS   Gumber HK, McKenna JF, Tolmie AF, Jalovec AM, Kartick AC, Graumann 
            K, Bass HW
  TITLE     MLKS2 is an ARM domain and F-actin-associated KASH protein that 
            functions in stomatal complex development and meiotic chromosome 
            segregation.
  JOURNAL   Nucleus. 2019 Dec;10(1):144-166. doi: 10.1080/19491034.2019.1629795.
  PUBMED    31221013
REFERENCE   2  (bases 1 to 12534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/19491034.2019"; journalName: "Nucleus"; date: "2019-12"; 
            volume: "10"; issue: "1"; pages: "144-166"
COMMENT     Sequence Label: MLKS2delTMexp
FEATURES             Location/Qualifiers
     source          1..12534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    28..52
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        745..1089
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1033..1053
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1189..1213
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1216..1218,1219..1221,1222..1932)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1942..1965
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1969..1995
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(3821..3845)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4217..4396
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             4432..5457
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      5655..5907
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6000..6024
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6545..7336
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7582..8170
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8071..8090
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8324..8341
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8356..8496
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8422..8441)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8582..8604
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8840..9034
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9100..10173)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10602..11231)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga
       61 tagtttaaac tgaaggcggg aaacgacaat ctgatccaag ctcaagctaa gcttgagctc
      121 tcccatatgg tcgactagag ccaagctgat ctcctttgcc ccggagatca ccatggacga
      181 ctttctctat ctctacgatc taggaagaaa gttcgacgga gaaggtgacg ataccatgtt
      241 caccaccgat aatgagaaga ttagcctctt caatttcaga aagaatgctg acccacagat
      301 ggttagagag gcctacgcgg caggtctcat caagacgatc tacccgagta ataatctcca
      361 ggagatcaaa taccttccca agaaggttaa agatgcagtc aaaagattca ggactaactg
      421 catcaagaac acagagaaag atatatttct caagatcaga agtactattc cagtatggac
      481 gattcaaggc ttgcttcata aaccaaggca agtaatagag attggagtct ctaagaaagt
      541 agttcctact gaatcaaagg ccatggagtc aaaaattcag atcgaggatc taacagaact
      601 cgccgtgaag actggcgaac agttcataca gagtctttta cgactcaatg acaagaagaa
      661 aatcttcgtc aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac
      721 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct
      781 cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg
      841 tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcccctgc
      901 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
      961 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
     1021 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
     1081 ggagaggact ccggtatttt tacaacaatt accacaacaa aacaaacaac aaacaacatt
     1141 acaatttact attctagtcg acctgcaggc ggccgcacta gtgatatcac aagtttgtac
     1201 aaaaaagcag gcttcatggt ctccaagggg gaggagctgt tcaccggggt ggtccccatc
     1261 ctggtggagc tggatggcga cgttaacggg cacaagttct cggtgtctgg cgagggggag
     1321 ggcgacgcta cctacgggaa gctcacgctg aagttcatct gcacgaccgg caagctgccg
     1381 gtgccctggc caacactcgt cacaactctg acttacggcg tccagtgctt ctcgcgctac
     1441 cctgatcaca tgaagcagca tgacttcttc aagtcggcta tgcccgaggg ctacgtgcag
     1501 gagcggacga tcttcttcaa ggacgatggc aactacaaga cacgcgcgga ggtcaagttc
     1561 gagggggaca ctctcgttaa caggatcgag ctgaagggca ttgacttcaa ggaggatggc
     1621 aatatcctcg ggcacaagct ggagtacaac tacaattccc ataacgtgta catcatggcc
     1681 gacaagcaga agaacggcat taaggtcaat ttcaagatcc ggcacaacat tgaggatggc
     1741 agcgttcagc tcgcggacca ttaccagcag aacacaccga tcggggatgg ccctgtcctc
     1801 ctgcccgaca atcactacct ctccactcag agcgccctgt cgaaggaccc gaacgagaag
     1861 agggaccata tggtgctcct ggagttcgtc acagccgcgg gcatcactct cgggatggat
     1921 gagctgtaca agggggcggg cgactacaag gacgatgacg ataaggctta cccctacgac
     1981 gtgccggatt acgccggggc tggcatgggg cgctcactct ccccgctcct gaggcaggag
     2041 ctggacaacc tggataagga cgccgattct cgcagggctg ccatgaaggc tctcaagtca
     2101 tacgcccgcc acctggatag caagtcgatc ccccatttcc tggccgaggt ctctgacacg
     2161 accgccgctg gcgcgggggc cggcagcggg atgccaaccg gcggggagtt cacgatctcg
     2221 ctctacgagg tcctggccag ggttcacggg cggaacatcg tgccccagat cggcaatatt
     2281 atggcgacca ttatgtgcac gctctccagc tcgggcgggt cattcccact gcaccaggcc
     2341 tgctccaagg ttgtgccagc tatcgctagg catggcattg atccgtcggc cccagacggc
     2401 gagaaggccg ggatcattgc tagcctctgc cgcccactgt gctcggctct catgggcggg
     2461 agccaggacg gcggcgctgc cagcggcgct gccctctgcc tgaaggccct ggttgagtct
     2521 tcaaactgga ggttcgcgtc gggcgagctg gtgaatgagg tctgcctgaa ggtggccggc
     2581 gctatgcacg actcggccac acggtcgaac gctcatatgg ggctggccat ggcgctcgtg
     2641 aagcacaatg gcctcatcgc tgaggcctac gcgcgctcta tcgtcaggtc cggcctccag
     2701 attctggacg gggataccgc tgactccagc tcgcagaaga ggctgtccgc catccagatg
     2761 attaacttct tcatgaagtt cgtggacccg aggtgcctgt cttcagagct gggccgggtt
     2821 atcgatgtga tggagcgctg ccagaatgac cggatgccct tcgtgcgggg cgcggctttc
     2881 gaggcctctc agtcagcgcg caacattgcc gcgcagaagg gctccaggca cgaggttggg
     2941 acctctccta tggtgggctc aaacttccat aagaggaggg agaagtcccc ttgccggagc
     3001 ctctggggcg ccaagaggtc ccccgctgcc tctacgatgg ccgctgctag ccctgtgcag
     3061 ttccgctctc cggagtcaca ggtcgttgat tccagcatca tgaatggctc cacactccac
     3121 gactccccag tgagcgtcgg ccagtcgtct tgcaacttcg accagagcag gcggactaac
     3181 cgcaggctgt ggtcgaatga cgcggttgat gtgtctctga aggacggcct cttcatccag
     3241 ctgtgctcca acagcaattc gtacgaggac gatctgggcg aggtttgcga tagcgaggtg
     3301 accgacgcta acttcgagtg caccaatacg ttcgccgggt tcgtctctcc ctcacacaat
     3361 ggctccatca gcagggataa gacgccttca ccgcgggcct cggacaggcc catcaacatt
     3421 gacgatgtga agatctactc cacgccaagg aagctcctgc ggctcctgca gtcatcctac
     3481 gactcggatt ctgcctcaaa catcgggcag agcacggcca agctcagggg cctctcctcc
     3541 cccgaccagg agcacaaggg cctcgtcatt tcttcagagc agatgcagtc gctgcattct
     3601 gacaataagg gcgatgagat gaaggacgag aacgagacca tctacatgca gaatagcaag
     3661 ggcaggacac agactctctc gaacgctgac gcctcagtcc tgtccacaac tgaggccgag
     3721 aacacaagca tcaagacttc gcctgagatt gagctgaatg gggacgatgt ttgcatcacc
     3781 tccagcattg gcaagacgcg gaagtaccgc gcgaagtgag acccagcttt cttgtacaaa
     3841 gtggtgatat cccgcggcca tgctagagtc cgcaaaaatc accagtctct ctctacaaat
     3901 ctatctctct ctatttttct ccagaataat gtgtgagtag ttcccagata agggaattag
     3961 ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt
     4021 atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtgacct
     4081 gcaggcatgc gacgtcgggc cctctagagg atccccgggt accgcgaatt atcgatcatg
     4141 agcggagaat taagggagtc acgttatgac ccccgccgat gacgcgggac aagccgtttt
     4201 acgtttggaa ctgacagaac cgcaacgttg aaggagccac tgagccgcgg gtttctggag
     4261 tttaatgagc taagcacata cgtcagaaac cattattgcg cgttcaaaag tcgcctaagg
     4321 tcactatcag ctagcaaata tttcttgtca aaaatgctcc actgacgttc cataaattcc
     4381 cctcggtatc caattagagt ctcatattca ctctcaactc gaatcccccc tatgaaaaag
     4441 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
     4501 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     4561 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     4621 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggagttc
     4681 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
     4741 cctgaaaccg aactgcccgc tgttcttcag ccggtcgcgg aggctatgga tgctatcgct
     4801 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
     4861 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
     4921 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
     4981 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     5041 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     5101 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     5161 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc aaggctccgg
     5221 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     5281 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     5341 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     5401 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagagt
     5461 agatgccgac cgaacaagag ctgatttcga gaacgcctca gccagcaact cgcgcgagcc
     5521 tagcaaggca aatgcgagag aacggcctta cgcttggtgg cacagttctc gtccacagtt
     5581 cgctaagctc gctcggctgg gtcgcgggag ggccggtcgc agtgattcag gaattaattc
     5641 cctagagtca agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     5701 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     5761 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     5821 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     5881 gcgcggtgtc atctatgtta ctagatcgac cggcatgcaa gctgataatt caattcggcg
     5941 ttaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg
     6001 tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc
     6061 acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc
     6121 gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag
     6181 ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac
     6241 agagcgttgc tgcctgtgat caattcgggc acgaacccag tggacataag cctgttcggt
     6301 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     6361 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
     6421 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     6481 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     6541 catcatgggg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     6601 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     6661 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     6721 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     6781 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     6841 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     6901 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     6961 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     7021 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     7081 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     7141 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     7201 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     7261 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
     7321 ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc ttcgcggcgc
     7381 ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa actatcaggt
     7441 caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat tgggagatat
     7501 atcatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7561 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7621 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7681 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     7741 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7801 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7861 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7921 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7981 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8041 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8101 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8161 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8221 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     8281 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     8341 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     8401 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     8461 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     8521 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     8581 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt
     8641 gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag
     8701 attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga
     8761 agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc
     8821 tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt
     8881 taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg
     8941 tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta
     9001 cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc gacctttttc
     9061 ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc
     9121 gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc
     9181 cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa
     9241 cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc
     9301 ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc
     9361 gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc
     9421 gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt
     9481 tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt
     9541 cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc
     9601 taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc
     9661 aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat
     9721 ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat
     9781 gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc
     9841 gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact
     9901 atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt
     9961 gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat
    10021 tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg
    10081 ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat
    10141 ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt cctcgggctt gggggttcca
    10201 gtgccattgc agggccggca gacaacccag ccgcttacgc ctggccaacc gcccgttcct
    10261 ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct
    10321 cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg
    10381 cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt
    10441 cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc
    10501 tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag
    10561 cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta
    10621 atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga
    10681 acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca
    10741 agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct
    10801 ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc
    10861 tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc
    10921 ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc
    10981 gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc
    11041 gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg
    11101 gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg
    11161 cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg
    11221 ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag
    11281 cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct
    11341 cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg
    11401 ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac acgtacccgg
    11461 ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct
    11521 ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc
    11581 ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc
    11641 ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc
    11701 ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg
    11761 ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct
    11821 ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa
    11881 caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc
    11941 cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag
    12001 gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc
    12061 cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc
    12121 ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc
    12181 atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc
    12241 gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag
    12301 ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagacggctg
    12361 cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca aagtacttta
    12421 aagtacttta aagtacttta aagtactttg atcccgaggg gaaccctgtg gttggcatgc
    12481 acatacaaat ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc gatt
//
