LOCUS       sequence-25592-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pCAG-RFP-miR-E2-4int
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           326..340
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 aaacatcgtg gaacagtcga gcgcaccgag ggccgccacc acctgttcct gtaattaatt
       61 aacaggtaag tgcgatcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc
      121 accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg
      181 ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc
      241 gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggta cctgctgttg acagtgagcg
      301 agaagtggtg tatctagaga ttatagtgaa gccacagatg tataatctct atacaccact
      361 tcctgcctac tgcctcggac ttcaagggga attcagggtg gggccacctg ccggtaggtg
      421 tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga
      481 atcgacaggc gccggacctc tggcggccgc aacaacgcgt tcctgaccat tcatcctctt
      541 tctttttcct gcaggcttgt ggaagaaatg ggaccctgct gctgctggag acggccactg
      601 ctccctgagt gtcccgtccc tggggctcct gcttccanca tcccggantt ttccctgctc
      661 cccacctcct gtcgtccggg ctgggcctcc agcccgantt tatcatcccc cggantnccn
      721 taaaaaaggc ngncctgaaa ncncnccccc cccccccgng nctttgggna ntgnacccaa
      781 aanncaanct gcccttgggg ttnccgaaac cnancc
//
