LOCUS       sequence-255922-       14206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of plant LINC fluorescent fusion protein for cell 
            biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14206)
  AUTHORS   McKenna JF, Gumber HK, Turpin ZM, Jalovec AM, Kartick AC, Graumann 
            K, Bass HW
  TITLE     Maize (Zea mays L.) Nucleoskeletal Proteins Regulate Nuclear 
            Envelope Remodeling and Function in Stomatal Complex Development and
            Pollen Viability.
  JOURNAL   Front Plant Sci. 2021 Feb 17;12:645218. doi: 
            10.3389/fpls.2021.645218. eCollection 2021.
  PUBMED    33679862
REFERENCE   2  (bases 1 to 14206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2021-02-17"; volume: "12:645218. doi"; pages: " 
            10.3389/fpls.2021.645218. eCollection 2021"
COMMENT     Sequence Label: NCH1exp
FEATURES             Location/Qualifiers
     source          1..14206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    37..61
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        754..1098
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1042..1062
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1198..1222
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1225..1227,1228..1230,1231..1941)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1951..1974
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1978..2004
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(5502..5526)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5898..6077
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             6113..7138
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      7336..7588
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7681..7705
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8226..9017
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9263..9851
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9752..9771
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10005..10022
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10037..10177
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10103..10122)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10263..10285
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10521..10715
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10781..11854)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12283..12912)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tatccgatta ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc
       61 aaacactgat agtttaaact gaaggcggga aacgacaatc tgatccaagc tcaagctaag
      121 cttgagctct cccatatggt cgactagagc caagctgatc tcctttgccc cggagatcac
      181 catggacgac tttctctatc tctacgatct aggaagaaag ttcgacggag aaggtgacga
      241 taccatgttc accaccgata atgagaagat tagcctcttc aatttcagaa agaatgctga
      301 cccacagatg gttagagagg cctacgcggc aggtctcatc aagacgatct acccgagtaa
      361 taatctccag gagatcaaat accttcccaa gaaggttaaa gatgcagtca aaagattcag
      421 gactaactgc atcaagaaca cagagaaaga tatatttctc aagatcagaa gtactattcc
      481 agtatggacg attcaaggct tgcttcataa accaaggcaa gtaatagaga ttggagtctc
      541 taagaaagta gttcctactg aatcaaaggc catggagtca aaaattcaga tcgaggatct
      601 aacagaactc gccgtgaaga ctggcgaaca gttcatacag agtcttttac gactcaatga
      661 caagaagaaa atcttcgtca acatggtgga gcacgacact ctcgtctact ccaagaatat
      721 caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc
      781 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga
      841 aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga
      901 tgcccctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
      961 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt
     1021 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
     1081 atttcatttg gagaggactc cggtattttt acaacaatta ccacaacaaa acaaacaaca
     1141 aacaacatta caatttacta ttctagtcga cctgcaggcg gccgcactag tgatatcaca
     1201 agtttgtaca aaaaagcagg cttcatggtc tccaaggggg aggagctgtt caccggcgtg
     1261 gtcccaatcc tcgtcgagct ggacggggat gttaacggcc acaagttctc cgtgagcggc
     1321 gagggggagg gcgacgccac ctacgggaag ctcacgctga agttcatttg cacgaccggc
     1381 aagctgccgg tgccctggcc aaccctggtc acaactctga cgtacggcgt ccagtgcttc
     1441 tcccgctacc cggaccacat gaagcagcat gatttcttca agagcgccat gccggagggc
     1501 tacgttcagg agcgcaccat cttcttcaag gacgatggca actacaagac aagggcggag
     1561 gttaagttcg agggcgacac tctcgtgaac cggatcgagc tgaagggcat tgacttcaag
     1621 gaggatggga atattctcgg ccacaagctg gagtacaact acaattcgca taacgtctac
     1681 atcatggctg acaagcagaa gaacggcatt aaggttaatt tcaagatccg ccacaatatt
     1741 gaggacggct ccgtgcagct cgcggatcat taccagcaga acacgccgat cggggacggc
     1801 cctgtcctcc tgccggataa tcactacctc tcgacgcagt ctgcgctgtc aaaggacccg
     1861 aacgagaaga gggatcatat ggttctcctg gagttcgtga cagccgcggg gatcactctc
     1921 ggcatggacg agctgtacaa gggggcgggc gactacaagg acgatgacga taaggcttac
     1981 ccctacgacg tgccagatta cgctggggcc ggcggatcca tgtttactcc gcagggcaaa
     2041 ggatggaccg gctggtcgac gccaactccg gccaaccagc ggagcggtgg tggggctcct
     2101 gctgcgtcgg cgccccttgg aaagggcaaa ggaagggtcg ccgagctcga gcaggagttg
     2161 catgaatatc agtacaatat ggggcttctt ttgattgaaa agaaagaatg ggcagcaaaa
     2221 tttgaagaaa tcagtgaagt gctgacacag aaagaggaga tactgaagag ggagcaggcg
     2281 gcacatctga atgctatatc agagtatgaa aggcgggagg agaacatgcg gaaagcacta
     2341 ggtgttgaga agcaatgtgt cgctgatctt gaaaaagcct tgcgtgatat acgagcagag
     2401 atagctgagg ttaaatttac atctgagaaa aagataacag atgctcagtc tcttgaggct
     2461 agtttggagg aaaaatcctt ggagatcgag ggaaaacttc atgctgcaga tgccaaactt
     2521 gcagaggcaa accgcaagaa gtctcaagct gaccgtgatt tagaagaggc ggaagctcgt
     2581 caacgtagac tggagaagga gaaactgtat tttgagacag agcggaaggc ccgagaaaag
     2641 caacttaagg agcaggagga atcactgcag gaatgggaga aaaagctgaa ggaaagtcag
     2701 aatagactta atgagttgca gagatctata aatgagcgag aggagagagc aaataagaat
     2761 gatcaacttt tcaagataaa acaggacgaa ctggaggagg cacgaaggac agtggaggcc
     2821 gctaaagtta ctctgaaagt caaagaggat gatattaaca aaagactaaa tgaattacat
     2881 ttacaggaaa aggatgctga ctcaaagcgc agtgcattgg aagagcaggg gaaaaaattg
     2941 gatgagagag aggcaaaggt cactaataga gaaaaggagg ggctccagaa gcttcttgag
     3001 gatcatcagg tggagcttga atccaaaaga cgggattttg aattagaatt agagagggaa
     3061 aggaaatctt ttgatcaaaa tatgacacag aagcaagcgg atttattgaa aagggagaaa
     3121 gatgttaaat ctttggaggc taaactttct aaaagtgagc aggcattgaa tgataagaag
     3181 aaatcaatgg aaaacttgca gaatgatctt gatgccaaat ctaaggctct gaagagttgg
     3241 gatgaatcat tgaaaaatga cgagaaaagg ttactgaaag aaaagcagca aatggatcat
     3301 gaaagagaac aattggagac atacaaattg gagcttgaaa agatcaaatc agcattagaa
     3361 gctgagaagg aaaaaatatc agaagagcag aataatctga aactcacagc gcaagagagg
     3421 caggagcata gcttgttgat agcaaaactt aagaaggaga ttgaggaata cagaatgcga
     3481 agcaactccc tatctgagga aatggaggat ttaaggaagc aacggcagaa atttgaagaa
     3541 gagtgggaac aactagacga aaagagagcc ctccttgtag aggaggacaa aaggttgaac
     3601 attgagagga tgaatttaga acggtggcgt gacaacgaag aaaaaaggtt aaatgatatg
     3661 aaactcaaaa tggatgaaga atacaagcag cagctggaga gtcttgagcg caaggaaaag
     3721 gctttgagtg atgatatgaa gcacaagcaa atggaaaatg atgaatttct gaagggagaa
     3781 cgtgctgatg ttcaacgtaa attgcaactc aaacggcatg aattggagat ggagatggag
     3841 caaaaacaag ccacgaagga gaaggaactg gaagaaaagg aaaatgagtt aaataagaaa
     3901 atagattttg tggaaaataa actccgacat gcaattgagt tgaatgagtc caagattgag
     3961 aagttattat tggagaaaag ggaggtgcag atggaaagag aattactgct ggaggaaaga
     4021 aagaaaacgg agactgacaa ggcagatatt agaagggata ttgagagtct gcactcattg
     4081 agcaagagtt tgaaggaacg tcgagaggca tataacaggg acaggagcag actcatcgaa
     4141 ctatttgaaa agtacaaggc gtgcaaaaac tgtgggattt ctatttttga gggattggat
     4201 tctctgctgc ttaaagacag tgctgaaatt gagcatccaa gtttggctgt tgagggagat
     4261 gatcatgcct tgaccaccga tacatcaggt ccagatacag gaacccttgt taattcaggt
     4321 ggtcgttttt ctttgttaca gaagtgttca aggcttttca agttctcccc tagaaaaaag
     4381 ggtgagcagt catcagaaca accatcagag aggaatatat cttttggtgc aagactcgag
     4441 gaagcaacac agagcgatgg agattatgtg ccaactcctg tctatgagat agcccacgat
     4501 tctttcaatg cagaggatga gctcccttct gacggtgaaa ccagggagaa tgaagagtct
     4561 gaaaggcatg acatagctga taatgcccag atggagtctt ctgttggtgt agcagataac
     4621 agtatagata ttctcggtac taagtcattt gatggtgcta acgacatggc agttgaagca
     4681 actattgtct ctgttgatca aaatggcgag gactctgtag ttcctgctga agcaggtgta
     4741 gagcctgaaa catcagagca aggtcggcgc cagcaaaacc agagaggcag aagaaaaggt
     4801 ggtatgaaaa gaacacggtc tgttaatgct gtagttgaag atgctaagat gatacttggc
     4861 gaggtttttg aggagaagac tgatgatcag ggagatacag tcaaggtggg tgctacacga
     4921 aagcggcggt ttgctggagc tacaataagt gaacaagatg aagaagggag cgaagcacat
     4981 tcagaaagtg tttcacttgg agggcagcgc cgtaagaggc ggcaaactgc tggggcagtg
     5041 acagatgccc caggagaaag acgttacaat ttgagacaca gtagagctgc aaatgctggc
     5101 gctgcaacag cccaagcaga caaaaaaaaa tcttcaaaag caagaaataa acatacagta
     5161 gaagcttctg ctgatgacac cgaaggaaca tcaaaggttg ttgaggaacc tgcccctgag
     5221 agcaagaggg tatctgaatc tgcagactac gatgcatccc agttgcatga gttttcccag
     5281 gttgaagttg gtgatgccca tgctgtagac ggtgagggtg ctggtgagga agatggtgat
     5341 attttggatg gccaagatgc actgcctgat gtgccaatga caccttctgg aagcgagttt
     5401 ggagcggaac aggaagatga ggacgatgat gattcagaga ggcgaaacca atcaatcagc
     5461 aagaagctat ggtcattctt cactacatga cctgcaggca gacccagctt tcttgtacaa
     5521 agtggtgata tcccgcggcc atgctagagt ccgcaaaaat caccagtctc tctctacaaa
     5581 tctatctctc tctatttttc tccagaataa tgtgtgagta gttcccagat aagggaatta
     5641 gggttcttat agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg
     5701 tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtgacc
     5761 tgcaggcatg cgacgtcggg ccctctagag gatccccggg taccgcgaat tatcgatcat
     5821 gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga caagccgttt
     5881 tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctgagccgcg ggtttctgga
     5941 gtttaatgag ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa gtcgcctaag
     6001 gtcactatca gctagcaaat atttcttgtc aaaaatgctc cactgacgtt ccataaattc
     6061 ccctcggtat ccaattagag tctcatattc actctcaact cgaatccccc ctatgaaaaa
     6121 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc
     6181 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg
     6241 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt
     6301 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggagtt
     6361 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct
     6421 gcctgaaacc gaactgcccg ctgttcttca gccggtcgcg gaggctatgg atgctatcgc
     6481 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca
     6541 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca
     6601 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct
     6661 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa
     6721 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg
     6781 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga
     6841 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc caaggctccg
     6901 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt
     6961 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac
     7021 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga
     7081 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagag
     7141 tagatgccga ccgaacaaga gctgatttcg agaacgcctc agccagcaac tcgcgcgagc
     7201 ctagcaaggc aaatgcgaga gaacggcctt acgcttggtg gcacagttct cgtccacagt
     7261 tcgctaagct cgctcggctg ggtcgcggga gggccggtcg cagtgattca ggaattaatt
     7321 ccctagagtc aagcagatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
     7381 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
     7441 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
     7501 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
     7561 cgcgcggtgt catctatgtt actagatcga ccggcatgca agctgataat tcaattcggc
     7621 gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt
     7681 gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg
     7741 cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc
     7801 cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa
     7861 gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga
     7921 cagagcgttg ctgcctgtga tcaattcggg cacgaaccca gtggacataa gcctgttcgg
     7981 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     8041 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     8101 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     8161 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     8221 acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     8281 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     8341 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     8401 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     8461 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     8521 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     8581 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     8641 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     8701 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     8761 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     8821 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     8881 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     8941 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     9001 aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg cttcgcggcg
     9061 cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg
     9121 tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata
     9181 tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     9241 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     9301 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     9361 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     9421 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     9481 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9541 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9601 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9661 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9721 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9781 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9841 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9901 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     9961 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    10021 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
    10081 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
    10141 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
    10201 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
    10261 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
    10321 tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta
    10381 gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg
    10441 aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg
    10501 ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt
    10561 ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt
    10621 gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt
    10681 acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt
    10741 cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt
    10801 cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct
    10861 ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga
    10921 acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg
    10981 cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat
    11041 cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct
    11101 cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct
    11161 ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt
    11221 tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt
    11281 ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg
    11341 caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca
    11401 tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca
    11461 tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct
    11521 cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac
    11581 tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct
    11641 tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga
    11701 ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc
    11761 gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga
    11821 tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc
    11881 agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc
    11941 tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc
    12001 tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct
    12061 gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct
    12121 tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt
    12181 ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta
    12241 gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt
    12301 aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag
    12361 aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc
    12421 aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc
    12481 tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg
    12541 ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac
    12601 cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg
    12661 cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt
    12721 cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg
    12781 ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc
    12841 gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc
    12901 gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca
    12961 gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc
    13021 tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg
    13081 gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg
    13141 gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc
    13201 tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg
    13261 cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt
    13321 cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg
    13381 cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg
    13441 gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc
    13501 tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca
    13561 acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc
    13621 ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca
    13681 ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg
    13741 ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc
    13801 cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg
    13861 catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt
    13921 cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca
    13981 gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct
    14041 gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt
    14101 aaagtacttt aaagtacttt aaagtacttt gatcccgagg ggaaccctgt ggttggcatg
    14161 cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaa
//
