LOCUS       sequence-256054-        9821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dox-dependent expression of mouse Tead4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9821)
  AUTHORS   Benchetrit H, Herman S, van Wietmarschen N, Wu T, Makedonski K, Maoz
            N, Yom Tov N, Stave D, Lasry R, Zayat V, Xiao A, Lansdorp PM, Sebban
            S, Buganim Y
  TITLE     Extensive Nuclear Reprogramming Underlies Lineage Conversion into 
            Functional Trophoblast Stem-like Cells.
  JOURNAL   Cell Stem Cell. 2015 Nov 5;17(5):543-56. doi: 
            10.1016/j.stem.2015.08.006. Epub 2015 Sep 24.
  PUBMED    26412562
REFERENCE   2  (bases 1 to 9821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2015.08.006"; journalName: "Cell Stem Cell"; date: 
            "2015-11-5- 5"; volume: "17"; issue: "5"; pages: "543-56"
COMMENT     Sequence Label: FUW-TetO-Tead4
FEATURES             Location/Qualifiers
     source          1..9821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        273..311
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     308..332
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    1869..2457
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1922..1942)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2340..2351)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2460..2476)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2461..2477)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2982..3162
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3188..3205)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3194..3418
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3464..3892
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3551..3570)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3761..3782
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3901..3921)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3906..4235
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4086..4221
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4148..4167
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4283..4330
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4349..4723
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4853..4974
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4890..4909)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4944..4963
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5023..5039)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5023..5039)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5036..5058)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5047..5063
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5071..5077,5078..5095,5096..5101))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5116..5137
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5254..5271)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5425..6013)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5505..5524)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6184..6975,6976..7044))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6807..6826
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7045..7149)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7224..7243)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7415..7794
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7796..7994
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8012..8192
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8239..8364
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8857..9090
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9275..9319
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9617..9734
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(9791..9809,9810..9821,1..11,12..30,31..53,54..72,
                     73..95,96..114,115..137,138..156,157..179,180..198,199..221,
                     222..240)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
       61 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
      121 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
      181 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
      241 aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata
      301 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
      361 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccccgaattc ggcttccatt
      421 acctccaacg agtggagctc tcccgactcc cccgagggga gcagcatctc tgggggcagc
      481 caggcactgg acaagcccat cgacaatgat gcagagggtg tatggagccc cgaaattgag
      541 cgaagcttcc aggaggccct ggccatctac ccaccctgcg gccgccgcaa aattatcctg
      601 acggaggaag gcaagatgta tggtcggaat gagctgatcg cacgccatat caagctcagg
      661 acagggaaga cgcgcacaag gaagcaggtc tccagccaca tccaggtgct tgcccgtcga
      721 aaagcccggg agatccaggc caaactcaag gaccaggcag ctaagaacaa ggccctgcag
      781 agcatggctg ccatgtcgtc cgcacagatc gtctcggcca cagccttcca cagtaaaatg
      841 gctcttgccc ggggccctgg ctacccagca atctcagggt tttggcaagg agctttgcca
      901 ggccaacctg gaacatccca cgatgtgaaa cctttctctc aaaacaccta ccctgtccag
      961 cctccgctgc ctctgccagg ctttgagtct cctgcagggc ccaccccctc accctccgca
     1021 ccgctcgctc ctccgtggca aggccgcagc atagctagct ccaagctctg gatgttggag
     1081 ttctcggctt tcctggagcg ccagcaagat cccgacacat acaacaaaca cctgtttgtg
     1141 cacatcagcc agtcaagccc aagctacagt gacccctacc tcgaaactgt ggacatccgc
     1201 caaatctatg acaagttccc agagaagaag ggaggcctca aggagctatt tgaacggggg
     1261 ccctctaatg ccttcttcct tgtgaagttc tgggcagacc tcaataccaa catcgatgac
     1321 gagggcagcg ccttctatgg ggtctccagc cagtacgaga gcccggagaa catgattatc
     1381 acctgctcta cgaaggtctg ctcatttggc aagcaagtgg tggagaaagt tgagacggag
     1441 tatgcccgct atgagaatgg ccactatttg taccgcatcc accggtcccc tctctgtgag
     1501 tacatgatca actttatcca caagctgaaa cacttacccg agaagtacat gatgaacagt
     1561 gtgctggaga acttcaccat cctgcaggtg gtcaccaacc gagacacaca ggagaccttg
     1621 ctgtgtattg catatgtctt cgaagtttca gccagcgagc atggggctca gcaccacatc
     1681 taccgacttg tgaaagaatg agagacttgg agagcaatgg taggggggac aagtccagga
     1741 catggggacc tgggagggga ccttcaggga cagtcccctg gaagtgccaa gagagccaag
     1801 cagagttgcc aggtctcttc ttaccaggag caaactgcca agccgaattc gatatcaagc
     1861 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     1921 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     1981 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     2041 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     2101 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     2161 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     2221 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     2281 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     2341 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     2401 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     2461 gataccgtcg acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     2521 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     2581 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     2641 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     2701 ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag
     2761 ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     2821 aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc
     2881 tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag
     2941 catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc
     3001 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     3061 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     3121 gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc
     3181 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     3241 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     3301 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     3361 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     3421 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     3481 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     3541 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     3601 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     3661 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     3721 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     3781 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     3841 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     3901 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     3961 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     4021 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     4081 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     4141 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     4201 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     4261 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
     4321 ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct
     4381 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
     4441 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
     4501 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
     4561 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
     4621 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
     4681 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt
     4741 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     4801 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     4861 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     4921 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     4981 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     5041 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     5101 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     5161 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     5221 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     5281 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5341 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5401 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5461 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5521 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5581 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     5641 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5701 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5761 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5821 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     5881 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     5941 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     6001 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6061 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     6121 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     6181 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     6241 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     6301 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     6361 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     6421 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     6481 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     6541 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     6601 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     6661 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6721 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6781 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     6841 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     6901 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     6961 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     7021 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     7081 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     7141 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
     7201 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     7261 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
     7321 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
     7381 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
     7441 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     7501 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     7561 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     7621 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     7681 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     7741 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     7801 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     7861 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     7921 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     7981 gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     8041 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     8101 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     8161 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     8221 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     8281 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     8341 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     8401 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     8461 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     8521 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     8581 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     8641 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     8701 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     8761 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     8821 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     8881 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     8941 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     9001 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     9061 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     9121 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     9181 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     9241 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     9301 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     9361 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     9421 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     9481 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     9541 cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg
     9601 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     9661 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     9721 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg ttagatctcg
     9781 agtttaccac tccctatcag tgatagagaa aagtgaaagt c
//
