LOCUS       sequence-256056-        4135 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Component of optically-induced motor dimerization system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Ross TD, Lee HJ, Qu Z, Banks RA, Phillips R, Thomson M
  TITLE     Controlling organization and forces in active matter through 
            optically defined boundaries.
  JOURNAL   Nature. 2019 Aug;572(7768):224-229. doi: 10.1038/s41586-019-1447-1. 
            Epub 2019 Aug 7.
  PUBMED    31391558
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-019-1447-1"; journalName: "Nature"; date: "2019-08";
            volume: "572"; issue: "7768"; pages: "224-229"
COMMENT     Sequence Label: K401-iLid
FEATURES             Location/Qualifiers
     source          1..4135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     203..225
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     290..309
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        290..308
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             340..362
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1603..2040
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF"
     CDS             2065..2082
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(2164..2955,2956..3024))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2787..2806
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3025..3129)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3197..3215
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      3344..3932
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3833..3852
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4086..4103
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccttacgcat ctgtgcggta tttcacaccg cataggaaga tcttccggaa gatcttccta
       61 tggtgcactc tcagtacaat ctgctctgat gcgctacgtg actgggtcat ggctgcgccc
      121 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
      181 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
      241 ccgaaacgcg cgaggcccag cgattcgaac ttctgataga cttcgaaatt aatacgactc
      301 actataggga gaccacaacg gtttccctct agaaataatt ttgtttaact ttaagaagga
      361 gatatacata tgtccgcgga acgagagatt cccgccgagg acagcatcaa agtggtctgc
      421 cgattccgac cgctgaacga cagcgaagag aaggccggct ccaagttcgt ggtcaagttc
      481 cccaacaatg tggaggagaa ctgcatatcc atagcgggca aggtgtattt gttcgacaag
      541 gtcttcaaac cgaatgcatc ccaggaaaag gtctacaatg aggcggccaa gtccattgtt
      601 acggatgtcc tggccgggta caatggaacg atattcgcat atggtcagac gtcctccgga
      661 aaaacgcata cgatggaggg cgtgatcggg gactccgtaa aacagggtat cataccacgt
      721 atcgtcaacg acattttcaa tcacatctac gcgatggagg tgaacctgga gtttcacatc
      781 aaggtctcct actacgagat ctacatggac aagattcgag atctgttgga cgtctccaag
      841 gtgaacctta gtgtgcacga ggataagaac cgggtgccgt acgtcaaggg cgctacggaa
      901 cggttcgtct cgtcgccgga ggatgttttc gaggtgatcg aggagggcaa atccaatcgt
      961 cacatcgctg tgacaaacat gaacgagcat tcttcgcgat cccactcagt attccttatc
     1021 aatgtgaagc aggagaacct ggagaaccag aagaaactat ccggcaaact ctacctggtg
     1081 gatttggccg gttccgagaa ggtttccaag actggagcgg agggaaccgt tcttgatgaa
     1141 gccaagaaca tcaacaagtc gctgtcggcc ttgggcaacg taatttctgc cctggcggac
     1201 ggaaacaaaa cgcacatccc ctaccgtgat tccaagctaa cgcgcatcct gcaggagtcg
     1261 ctgggaggca acgcacgcac aaccatcgtc atctgctgct ctccagccag tttcaacgaa
     1321 tctgaaacga agtcaacgct ggacttcggt cgtagagcca agacagtgaa gaacgtggtc
     1381 tgcgttaacg aggagcttac tgccgaggaa tggaagcgac gctatgaaaa ggagaaggaa
     1441 aagaacgccc gactaaaggg taaggtggag aagctggaga tcgagcttgc gcgctggaga
     1501 gcgggtgaaa ctgttaaggc ggaggagcaa atcaacatgg aggatctcat ggaggcaagc
     1561 acgcccaacc tgcgcaaggc cggtagtggt agtggggagt ttctggcaac cacactggaa
     1621 cggatcgaga aaaatttcgt gattactgat ccgagactgc ctgacaaccc aatcattttt
     1681 gcgagcgatt ccttcctgca gctgacagaa tattctcggg aagagatcct ggggcgcaat
     1741 tgccgttttc tgcagggacc cgagacagac cgtgccactg ttcggaaaat cagagatgct
     1801 attgacaacc agactgaagt gaccgttcag ctgatcaatt ataccaagag cggcaagaag
     1861 ttctggaacg tgttccacct gcagccgatg cgcgattata agggcgacgt ccagtacttc
     1921 attggcgtgc agctggatgg caccgaacgt cttcatggcg ccgctgagcg tgaggcggtc
     1981 tgcctgatca aaaagacagc ctttcagatt gctgaggcag cgaacgacga aaattacttt
     2041 ggaagcgggt ctgagactag tgggcaccac catcaccatc actgacaagc ttatcgatga
     2101 taagctgtca aacatgagaa ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     2161 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     2221 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     2281 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     2341 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     2401 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     2461 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     2521 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     2581 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     2641 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     2701 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     2761 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt
     2821 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     2881 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     2941 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     3001 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     3061 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     3121 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     3181 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aataaaagga
     3241 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3301 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3361 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3421 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3481 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3541 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3601 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3661 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3721 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3781 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3841 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3901 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3961 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4021 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     4081 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttct
//
