LOCUS       sequence-256084-        5031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the voltage sensor Arch(D95N) in the pharynx of C. 
            elegans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Azimi Hashemi N, Bergs ACF, Schuler C, Scheiwe AR, Steuer Costa W, 
            Bach M, Liewald JF, Gottschalk A
  TITLE     Rhodopsin-based voltage imaging tools for use in muscles and neurons
            of Caenorhabditis elegans.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Aug 1. pii: 1902443116. doi: 
            10.1073/pnas.1902443116.
  PUBMED    31371514
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2019 Aug 1. pii: 1902443116. doi: 
            10.1073/pnas.1902443116."
COMMENT     Sequence Label: pNH11 (pmyo-2::Arch(D95N)::2xMycTag)
FEATURES             Location/Qualifiers
     source          1..5031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      568..1156
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1057..1076
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     regulatory      2386..2395
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2392..3165
                     /codon_start=1
                     /product="light-driven proton pump archaerhodopsin-3 from 
                     Halorubrum sodomense (Chow et al., 2010)"
                     /label=Arch
                     /translation="MDPIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT
                     DKDAREYYAVTILVPGIASAAYLSMFFGIGLTEVTVGGEMLDIYYARYANWLFTTPLLL
                     LDLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYFLA
                     TSLRSAAKERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLD
                     VTAKVGFGFILLRSRAILGDTEAPEPSAGADVSAAD"
     CDS             3175..3204
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3208..3237
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4154..4176)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4276..4295
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4377..4395)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4463..4567
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4568..4636,4637..5031,1..397)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4786..4805)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
       61 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      121 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      181 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      241 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      301 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      361 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
      421 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
      481 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      541 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      601 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      661 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
      721 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      781 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
      841 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
      901 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
      961 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1021 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1081 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1141 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1201 gccttttgct cacatgtcag gtcgaggcat ttgaattggg ggtggtggac agtaactgtc
     1261 tgtaataata attactcctg accaggttgc aattcgagtt ttgataagca taattatacc
     1321 ttgtacattg tgggttttgt gctgtggacg ttttattgtg acatccccat aagctacaag
     1381 aacaaaaatg aaatgtaaaa gtattgaaaa cgtcgtacat tttatatctg agtagtatcc
     1441 tttgctttaa atgtccataa aactaatttt ataatcaata aaacaacgtt tgtaaatcaa
     1501 ctgagtttac aagtagagac attgagggat actttcacta tgctaaagtg ataatcgacc
     1561 aaataataac ttcactttgg tatttattcc tgtcttataa atgttatgta tgaattaaat
     1621 tcatatgcat atggctcact ctgacaattt ttaataatct tccagatcaa tattgactac
     1681 cgatgcgggt ggtcttttgc tttgaattct gctgaacttt acaccccgaa cagcaatgtg
     1741 tgcttcagcc taaaaaaaag taagtgtgtt aatcagtgcc cccgattctt cattttttgc
     1801 ccctctctcc cgtttcgtcg gcaaaagaag agaaaataaa gataagtctc aagataggtt
     1861 ggtaatcgct aaagtggttg tgtggataag agtagcaaaa tggcaggaag agcactttgc
     1921 gcgcacacac tgtactcatt gttctggata aaattctctc gttgtttgcc gtcggatgtc
     1981 tgcctctctg cattgagccg gcttcttcac tatctttagt taacctaaaa tgccgtttct
     2041 tttctcgtat ccccactatc ccgttgaggt tctctgctct cttcgctccc taccgccagc
     2101 gagcaactat ccgtgggggc gccttgctcg gaagatgggg gggaagaaag aagatttttg
     2161 ctatttgcac ttgagaaaga gacttttcct gcgtcgatgg ttagagaaca gtgtgcagac
     2221 acttttcagc tacctagaat tacaattgga tatccccgcc tcccaatcca cccacccagg
     2281 gaaaaagaag ggctcgccga aaatcaaagt tatctccagg ctcgcgcatc ccaccgagcg
     2341 gttgacttct ctccaccact tttcatttta accctcgggg gatccgccac catggacccc
     2401 atcgctctgc aggctggtta cgacctgctg ggtgacggca gacctgaaac tctgtggctg
     2461 ggcatcggca ctctgctgat gctgattgga accttctact ttctggtccg cggatgggga
     2521 gtcaccgata aggatgcccg ggaatattac gctgtgacta tcctggtgcc cggaatcgca
     2581 tccgccgcat atctgtctat gttctttggt atcgggctta ctgaggtgac cgtcgggggc
     2641 gaaatgttgg atatctatta tgccaggtac gccaactggc tgtttaccac cccacttctg
     2701 ctgctggatc tggcccttct cgctaaggtg gatcgggtga ccatcggcac cctggtgggt
     2761 gtggacgccc tgatgatcgt cactggcctc atcggagcct tgagccacac ggccatagcc
     2821 agatacagtt ggtggttgtt ctctacaatt tgcatgatag tggtgctcta ttttctggct
     2881 acatccctgc gatctgctgc aaaggagcgg ggccccgagg tggcatctac ctttaacacc
     2941 ctgacagctc tggtcttggt gctgtggacc gcttacccta tcctgtggat cataggcact
     3001 gagggcgctg gcgtggtggg cctgggcatc gaaactctgc tgtttatggt gttggacgtg
     3061 actgccaagg tcggctttgg ctttatcctg ttgagatccc gggctattct gggcgacacc
     3121 gaggcaccag aacccagtgc cggtgccgat gtcagtgccg ccgaccgacc ggtagagcag
     3181 aaattgatca gcgaggaaga cttgggcgaa caaaagctta tttctgaaga agacttgtga
     3241 attccaactg agcgccggtc gctaccatta ccaacttgtc tggtgtcaaa aataataggg
     3301 gccgctgtca tcagagtaag tttaaactga gttctactaa ctaacgagta atatttaaat
     3361 tttcagcatc tcgcgcccgt gcctctgact tctaagtcca attactcttc aacatcccta
     3421 catgctcttt ctccctgtgc tcccaccccc tatttttgtt attatcaaaa aacttctctt
     3481 aatttctttg ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca
     3541 aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat
     3601 aataattcta tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttacttc
     3661 tgataaattt ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt atcatatgtt
     3721 acgtttcagt ttatgaccgc aatttttatt tcttcgcacg tctgggcctc tcatgacgtc
     3781 aaatcatgct catcgtgaaa aagttttgga gtatttttgg aatttttcaa tcaagtgaaa
     3841 gtttatgaaa ttaattttcc tgcttttgct ttttggggtt tcccctattg tttgtcaaga
     3901 tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa
     3961 gatcggaaga aggtttgggt ttgaggctca gtggaaggtg agtagaagtt gataatttga
     4021 aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca gaatacattc ccaatatacc
     4081 aaacataact gtttcctact agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg
     4141 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     4201 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     4261 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     4321 aaataccgca cagatgcgta aggagaaaat accgcatcag gcggccttaa ttaagggcct
     4381 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     4441 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     4501 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     4561 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     4621 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     4681 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     4741 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     4801 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     4861 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     4921 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     4981 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg a
//
