LOCUS       sequence-256085-        2346 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Biosensor for monitoring activation for rice small GTPase OsRac1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Wong HL, Akamatsu A, Wang Q, Higuchi M, Matsuda T, Okuda J, Kosami 
            KI, Inada N, Kawasaki T, Kaneko-Kawano T, Nagawa S, Tan L, Kawano Y,
            Shimamoto K
  TITLE     In vivo monitoring of plant small GTPase activation using a Forster 
            resonance energy transfer biosensor.
  JOURNAL   Plant Methods. 2018 Jul 7;14:56. doi: 10.1186/s13007-018-0325-4. 
            eCollection 2018.
  PUBMED    30002723
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2018-07-7"; volume: "14:56. doi"; pages: " 
            10.1186/s13007-018-0325-4. eCollection 2018"
COMMENT     Sequence Label: Raichu-OsRac1 WT
FEATURES             Location/Qualifiers
     source          1..2346
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(10..12,13..15,16..528)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             join(1531..1533,1534..1536,1537..2049)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIGFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
ORIGIN
        1 gaattcggca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
       61 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      121 gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc
      181 tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg ctaccccgac
      241 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      301 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      361 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
      421 ctggggcaca agctggagta caactacaac agccacaacg tctatatcac cgccgacaag
      481 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg
      541 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
      601 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
      661 cacatggtcc tgctggagtt cgtgaccgcc gccctcgaga aagagaaaga gcggccagag
      721 atttctctcc cttcagattt tgaacacaca attcatgtcg gttttgatgc tgtcacaggg
      781 gagtttacgg gaatgccaga gcagtgggcc cgcttgcttc agacatcaaa tatcactaag
      841 tcggagcaga agaaaaaccc gcaggctgtt ctggatgtgt tggagtttta caactcgaag
      901 aagacatcca acagccagaa atacatgagc tttacagata agtcagcttc cggaggtgga
      961 accggtggtg gaggtaccat gagctcggcg gcggcggcga cgaggttcat caagtgcgtc
     1021 accgtggggg acggcgcggt ggggaagacg tgcatgctca tctgctacac ctgcaacaag
     1081 ttccccaccg attacatccc caccgtgttc gacaacttca gcgccaatgt ctccgtggac
     1141 gggagcgtcg tcaacctcgg cctctgggac actgcaggtc aggaggatta cagcaggttg
     1201 aggcctctga gctacagggg agccgatgtg ttcatcctgt ccttctccct gataagcagg
     1261 gcgagctatg agaatgttca gaagaagtgg atgccagagc ttcgccggtt tgcgcctggt
     1321 gttcctgtag ttcttgttgg aaccaagttg gatctccgtg aagatagggc ctatcttgct
     1381 gatcatccag cttcttccat aataacaacg gagcagggag aagaactgag gaagctaata
     1441 ggagcggtcg cctacatcga atgcagctcc aagacacaga gaaacattaa agctgttttc
     1501 gacactgcca tcaaagtggt gcgcggccgc atggtgagca agggcgagga gctgttcacc
     1561 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacag gttcagcgtg
     1621 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     1681 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag
     1741 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1801 gaaggctacg tccaggagcg taccatcttc ttcaaggacg acggcaacta caagacccgc
     1861 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcggc
     1921 ttcaaggagg acggcaacat cctggggcac aagctagagt acaactacat cagccacaac
     1981 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg cccacttcaa gatccgccac
     2041 aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     2101 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     2161 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     2221 actctcggca tggacgagct gtctagacca agacataagg atgtaaccag aaagaaactc
     2281 caatcaagct ccaatcggcc agtaaggcgg tacttttgcg gaagcgcttg tttcgcgtag
     2341 ggatcc
//
