LOCUS       sequence-256134-       10364 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dual yTRAP, Gateway destination vector of aTF2 fusion reporting on 
            mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10364)
  AUTHORS   Newby GA, Kiriakov S, Hallacli E, Kayatekin C, Tsvetkov P, Mancuso 
            CP, Bonner JM, Hesse WR, Chakrabortee S, Manogaran AL, Liebman SW, 
            Lindquist S, Khalil AS
  TITLE     A Genetic Tool to Track Protein Aggregates and Control Prion 
            Inheritance.
  JOURNAL   Cell. 2017 Nov 2;171(4):966-979.e18. doi: 
            10.1016/j.cell.2017.09.041. Epub 2017 Oct 19.
  PUBMED    29056345
REFERENCE   2  (bases 1 to 10364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.041"; journalName: "Cell"; date: "2017-11-2-
            2"; volume: "171"; issue: "4"; pages: "966-979.e18"
COMMENT     Sequence Label: pSK247
FEATURES             Location/Qualifiers
     source          1..10364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..188)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     109..133
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     regulatory      655..664
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1735..1824)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             complement(1831..1920)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             complement(2524..2544)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    2582..2706
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2747..3052)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(2849..2868)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(3394..4053)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3968..3987
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(4107..4113,4114..4131,4132..4137))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(4162..4286)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(4839..5248)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(5543..5559)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5543..5559)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5556..5578)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5567..5583
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5638..5659
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5776..5793)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5947..6535)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6027..6046)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6706..7497,7498..7566))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7329..7348
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7567..7671)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7739..7757
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7795..7817)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7917..7936
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8090..8111)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8224..8243
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8528..8550
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8542..8559
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8543..8559
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8566..8585
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8566..8584
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8599..8615)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8599..8615)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(9132..9941)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9855..9874
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ccggtagagg tgtggtcaat aagagcgacc tcatactata cctgagaaag caacctgacc
       61 tacaggaaag agttactcaa gaataagaat tttcgtttta aaacctaaga gtcactttaa
      121 aatttgtata cacttatttt ttttataact tatttaataa taaaaatcat aaatcataag
      181 aaattcgctt atttagaagt ggcgcgcctt acttgtacag ctcgtccatg cccatcacat
      241 cggtaaaggc cttttgccac tccttgaagt tgagctcggt cttggagtgc ttgagctccg
      301 tcttacggaa cacgtacatc ggctggttct tcagatagtt agccgccatt ggcttggcaa
      361 aggtgtaggt ggtccgcgca gtgctccggt agcgcttgcc atttccagtg gtgtaactcc
      421 acttaaaggt actgatgatg gttttgtcgt tggggtaagt cttcttcgac ctgcaccagt
      481 ccgcagcggt cagcgagttg gtcatcacag gaccgtcagc agggaaacca gtccccttca
      541 cctgggcctc tcctttgatg tggcttccct cgtaggtgta gcggtagtta acagtaaggg
      601 aggcaccatc ttcaaactgc attgtgcgat ggacttggta tccggagcca tctaccatgg
      661 cggcctggaa aggcgacatc ccgtcagggt agggcaggta ctgatggaag ccatacccga
      721 tatgagggac cagaatccag ggggagaact ggaggtcacc cttggtggac ttcaggttta
      781 actcctcata accatcattt ggattgccgg tgccctgacc caccatgtca aagtccacac
      841 cgttgatgga gccaaagatg tgtaactcat gtgtcgctgg gagagaggcc atgttatcct
      901 cctcgcccat tttttgttta aacagtgtgt gtatttgtgt ttgtgtgtct atagaagtat
      961 agtaatttat gctgcaaagg tcctaatgta taaggaaaga atatttagag aaaagaagaa
     1021 aacaagagtt ttatatacat acagagcaca tgcatgccat atgatcatgt gtcgtcgcac
     1081 acatatatat atgcctgtat gtgtcagcac taaagttgcc tggccatcca cgctatatat
     1141 acacgcctgg cggatctggg ccggccacac gtcctaactt ctaaaacacg tcctcagata
     1201 attacacgtc ctcagattta ggacacgtcc tcaatgcttt aaacacgtcc tcatatttaa
     1261 tacacgtcct cactttagaa ccacacgtcc tcaattaaac tacacgtcct cattaattaa
     1321 agaaaatacc gcatcaggaa attgtaaacg ttaatatttt gttaaaattc gcgttaaatt
     1381 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     1441 caaaagaata gaccgagata gggttgagtg atgaaaacgt gattgaagga gttgaaacct
     1501 tgctagatat ttttatgaaa ttctagatat attgagaggt gaagtttacc ttgtttatgg
     1561 tatatggtac aaaaagaact aaactaatta tacgtctata tatatatata tatatataac
     1621 agctttatta aaccttgttt tttaatatag aagaaaatgc tttatgatcg gtattattgt
     1681 gtttgcattt acttatgttt gcaagaaatt acatgagggc cctcattatg aagaagcgta
     1741 atctgggacg tcgtatgggt aactaccagc gtagtctggt acgtcatatg gataaccggc
     1801 gtaatcaggg acgtcataag gatatcttga ggcataatca ggtacatcgt atggataaga
     1861 acctgcgtag tcaggaacat cgtaagggta accagcataa tctggaacat catatgggta
     1921 tctagcagca gaaccagaat tgatatccaa accagccaat ttcaaagcca attcgccggc
     1981 agaacctctc aggtgggttt ttaggtgtct agtcaattga tcatgtctac tgaagttgcg
     2041 catgcatatt cggcattgga atggcttctc gccggtgtgc gtacgtagat gtcttctcaa
     2101 attagctcta tcggagaaat ttcgcataca gatccgacac tgaaacggtt tttcaccggt
     2161 atgagtacgg gtatgtcttt gcaaaatgaa atttctcgaa aagttccgca tgcaaatgcg
     2221 acactggaag gggcgctccc cgggtctagc accaccacca tattcatcaa tacccaaggc
     2281 atcggtgaac atttgttcga attcaaagtc agccatatct aaagcaccat atggagcaga
     2341 atcatgtgga gtaaaacctg gacctggaga atcaccgtca cccaacatat ccaaatcaaa
     2401 atcatccaaa gcatcagcat gagccatagc aacatcttca ccatccaaat gcaattcatc
     2461 acccaaagaa acatcagttg gtggagcttg taaaccacca tcaactggaa caccatgaat
     2521 accaaccttt ctcttcttct ttggagccat taagcttgat atcgaattcc tgcagcccat
     2581 caccactttg tacaagaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
     2641 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
     2701 actatggtcg acctgcagac tggctgtgta taagggagcc tgacatttat attccccaga
     2761 acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt
     2821 ccccgataac ggagaccggc acactggcca tatcggtggt catcatgcgc cagctttcat
     2881 ccccgatatg caccaccggg taaagttcac gggagacttt atctgacagc agacgtgcac
     2941 tggccagggg gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc tgtacatcca
     3001 caaacagacg ataacggctc tctcttttat aggtgtaaac cttaaactgc atttcaccag
     3061 cccctgttct cgtcagcaaa agagccgttc atttcaataa accgggcgac ctcagccatc
     3121 ccttcctgat tttccgcttt ccagcgttcg gcacgcagac gacgggcttc attctgcatg
     3181 gttgtgctta ccagaccgga gatattgaca tcatatatgc cttgagcaac tgatagctgt
     3241 cgctgtcaac tgtcactgta atacgctgct tcatagcata cctctttttg acatacttcg
     3301 ggtatacata tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat acgcatactg
     3361 ttatctggct tttagtaagc cggatccacg cgtttacgcc ccgccctgcc actcatcgca
     3421 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
     3481 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
     3541 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     3601 cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc
     3661 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     3721 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     3781 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacggaattc
     3841 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     3901 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     3961 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     4021 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     4081 cgacggatcc taactcaaaa tccacacatt atacgagccg gaagcataaa gtgtaaagcc
     4141 tggggtgcct aatgcggccg ccatagtgac tggatatgtt gtgttttaca gtattatgta
     4201 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct
     4261 cgttcagctt ttttgtacaa acttgtgatg gggctagctg ttgctagtgg gcagatatag
     4321 atgttattcc gagcaagtcg atgaagaaac cgctttttgt tacagtacaa tggagtcttt
     4381 caagagaaga tgtaccaata tacactacac tcttcagaag caatgggagc tttggtcgag
     4441 tgaaaaaaaa attttctcca taaagaaaga tcatattata cgatgatgta agatataata
     4501 cccggttgta atgtacattt aagagcaagg taagaagtga caataacttc tgtatgatct
     4561 tagcatgtac ctcttttggt gggctgagaa ctaagattca tctttttgcg gaagaatttt
     4621 gctatgaact tcacaacttt atgaagtggt ttaagagaat tacaaaagaa atgacacaga
     4681 ctcgaacact gtgacgcgtc gtcttagtaa aaaataataa tttgagtcaa atagcgcagc
     4741 taatgcgaaa caaagaaatg aagcatatac cattcgttgt atgatttttg tgtggttgca
     4801 ctcggtgcag cgtacgacgc catcttaggg gcagacatta gaatggtata tccttgnaan
     4861 atatatatat atatattgct gaaatgtaaa aggtaagaaa agttagaaag taagacgatt
     4921 gctaaccacc tattggaaaa aacaataggt ccttaaataa tattgtcaac ttcaagtatt
     4981 gtgatgcaag catttagtca tgaacgcttc tctattctat atgaaaagcc ggttccggcg
     5041 ctctcacctt tcctttttct cccaattttt cagttgaaaa aggtatatgc gtcaggcgac
     5101 ctctgaaatt aacaaaaaat ttccagtcat cgaatttgat tctgtgcgat agcgcccctg
     5161 tgtgttctcg ttatgttgag gaaaaaaata atggttgcta agagattcga actcttgcat
     5221 cttacgatac ctgagtattc ccacagttaa ctgcggtcaa gatatttctt gaatcaggcg
     5281 ccttagaccg ctcggccaaa caaccaatta cttgttgaga aatagagtat aattatccta
     5341 taaatataac gtttttgaac acacatgaac aaggaagtac aggacaattg attttgaaga
     5401 gaatgtggat tttgatgtaa tcgttgggat tccattttta ataaggcaat aatattaggt
     5461 atgtagatat actagaagtt ctcctcggcg gccgccacgg cgatatcgga tctcgaggcc
     5521 tcggactagt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     5581 caattccaca caacatacga gccgcggaag cataaagtgt aaagcctggg gtgcctaatg
     5641 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5701 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5761 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5821 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5881 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5941 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6001 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6061 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6121 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6181 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6241 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6301 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6361 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     6421 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6481 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6541 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6601 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6661 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6721 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6781 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6841 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6901 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6961 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     7021 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     7081 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     7141 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     7201 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     7261 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     7321 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     7381 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     7441 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     7501 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     7561 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     7621 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7681 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     7741 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     7801 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     7861 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     7921 atcagagcag attgtactga gagtgcacca taaaattgta aacgttaata ttttgttaaa
     7981 attcgcgtta aatttttgtt aaatcagctc attttttaac caatagaccg aaatcggcaa
     8041 aatcccttat aaatcaaaag aatagcccga gatagagttg agtgttgttc cagtttggaa
     8101 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     8161 gggcgatggc ccaccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     8221 ggaagggaag aaagcgaaag gagcgggcgc taaggcgctg gcaagtgtag cggtcacgct
     8281 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt actatggttg
     8341 ctttgacgta tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     8401 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     8461 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     8521 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
     8581 tagggcgaat tggggatcga tccactagtt ctagagcggc cgcctacgtc gttaaggccg
     8641 tttctgacag agtaaaattc ttgagggaac tttcaccatt atgggaaatg gttcaaggag
     8701 gtattgactt aaactccatc aaatggtcag gtcattgagt gttttttatt tgttgtattt
     8761 tttttttttt agagaaaatc ctccaatata taaattagga atcatagttt catgattttc
     8821 tgttacacct aactttttgt gtggtgccct cctccttgtc aatattaatg ttaaagtgca
     8881 attctttttc cttatcacgt tgagccatta gtatcaattt gcttacctgt attcctttac
     8941 atcctccttt ttctccttct tgataaatgt atgtagattg cgtatatagt ttcgtctacc
     9001 ctatgaacat attccatttt gtaatttcgt gtcgtttcta ttatgaattt catttataaa
     9061 gtttatgtac aaatatcata aaaaaagaga atctttttaa gcaatctttt tattgtcagt
     9121 actgaagatc tttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     9181 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     9241 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     9301 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     9361 tgagtgacga ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt
     9421 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     9481 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
     9541 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     9601 gaatcaggat attcttctaa tacctggaat gctgttttgc cggggatcgc agtggtgagt
     9661 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     9721 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     9781 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
     9841 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     9901 tttaatcgcg gcctcgaaac gtgagtcttt tccttaccca tggttgtttc aattgatgtt
     9961 cggatgtgat gtgagaactg tatcctagca agattttaaa aggaagtata tgaaagaaga
    10021 acctcagtgg caaatcctaa ccttttatat ttctctacag gggcgcggcg tggggacaat
    10081 tcaacgcgtc tgtgagggga gcgtttccct gctcgcaggt ctgcagcgag gagccgtaat
    10141 ttttgcttcg cgccgtgcgg ccatcaaaat gtatggatgc aaatgattat acatggggat
    10201 gtatgggcta aatgtacggg cgacagtcac atcatgcccc tgagctgcgc acgtcaagac
    10261 tgtcaaggag ggtattctgg gcctccatgt cgctggccgg gtgacccggc ggggacgagg
    10321 caagctaaac gatctatatt accctgttat ccctagcgga tctg
//
