LOCUS       sequence-256137-        8797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for overexpression of prion domains to induce prions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Newby GA, Kiriakov S, Hallacli E, Kayatekin C, Tsvetkov P, Mancuso 
            CP, Bonner JM, Hesse WR, Chakrabortee S, Manogaran AL, Liebman SW, 
            Lindquist S, Khalil AS
  TITLE     A Genetic Tool to Track Protein Aggregates and Control Prion 
            Inheritance.
  JOURNAL   Cell. 2017 Nov 2;171(4):966-979.e18. doi: 
            10.1016/j.cell.2017.09.041. Epub 2017 Oct 19.
  PUBMED    29056345
REFERENCE   2  (bases 1 to 8797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.041"; journalName: "Cell"; date: "2017-11-2-
            2"; volume: "171"; issue: "4"; pages: "966-979.e18"
COMMENT     Sequence Label: pGAN101
FEATURES             Location/Qualifiers
     source          1..8797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(63..278)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(302..321)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     421..443
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(481..499)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      540..1882
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    872..919
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1909..2013
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2014..2082,2083..2874)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2232..2251)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3045..3633
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3534..3553
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3787..3804
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3921..3942
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3957..3962,3963..3980,3981..3987)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3995..4011
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4000..4022
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4019..4035
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4019..4035
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4054..4074
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4056..4074
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4098..4539
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    4098..4215
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     4510..4533
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     4554..4570
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4554..4570
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    4578..4702
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4727..4732,4733..4750,4751..4757)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4811..5470
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4877..4896)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5812..6117
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5996..6015
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6158..6282)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             6314..7012
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     terminator      7019..7266
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(7035..7053)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(7284..7303)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7285..7303)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7313..7330)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7313..7329)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7322..7344)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7470..7925
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7557..7576)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7767..7788
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(8056..8797,1..62))
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt agctttcgac
       61 atgatttatc ttcgtttcct gcaggttttt gttctgtgca gttgggttaa gaatactggg
      121 caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag tctgtgctcc
      181 ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca aggaaaccga
      241 aatcaaaaaa aagaataaaa aaaaaatgat gaattgaaaa ggtggtatgg tgcactctca
      301 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
      361 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
      421 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
      481 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagtatg
      541 atccaatatc aaaggaaatg atagcattga aggatgagac taatccaatt gaggagtggc
      601 agcatataga acagctaaag ggtagtgctg aaggaagcat acgatacccc gcatggaatg
      661 ggataatatc acaggaggta ctagactacc tttcatccta cataaataga cgcatataag
      721 tacgcattta agcataaaca cgcactatgc cgttcttctc atgtatatat atatacaggc
      781 aacacgcaga tataggtgcg acgtgaacag tgagctgtat gtgcgcagct cgcgttgcat
      841 tttcggaagc gctcgttttc ggaaacgctt tgaagttcct attccgaagt tcctattctc
      901 tagaaagtat aggaacttca gagcgctttt gaaaaccaaa agcgctctga agacgcactt
      961 tcaaaaaacc aaaaacgcac cggactgtaa cgagctacta aaatattgcg aataccgctt
     1021 ccacaaacat tgctcaaaag tatctctttg ctatatatct ctgtgctata tccctatata
     1081 acctacccat ccacctttcg ctccttgaac ttgcatctaa actcgacctc tacatttttt
     1141 atgtttatct ctagtattac tctttagaca aaaaaattgt agtaagaact attcatagag
     1201 tgaatcgaaa acaatacgaa aatgtaaaca tttcctatac gtagtatata gagacaaaat
     1261 agaagaaacc gttcataatt ttctgaccaa tgaagaatca tcaacgctat cactttctgt
     1321 tcacaaagta tgcgcaatcc acatcggtat agaatataat cggggatgcc tttatcttga
     1381 aaaaatgcac ccgcagcttc gctagtaatc agtaaacgcg ggaagtggag tcaggctttt
     1441 tttatggaag agaaaataga caccaaagta gccttcttct aaccttaacg gacctacagt
     1501 gcaaaaagtt atcaagagac tgcattatag agcgcacaaa ggagaaaaaa agtaatctaa
     1561 gatgctttgt tagaaaaata gcgctctcgg gatgcatttt tgtagaacaa aaaagaagta
     1621 tagattcttt gttggtaaaa tagcgctctc gcgttgcatt tctgttctgt aaaaatgcag
     1681 ctcagattct ttgtttgaaa aattagcgct ctcgcgttgc atttttgttt tacaaaaatg
     1741 aagcacagat tcttcgttgg taaaatagcg ctttcgcgtt gcatttctgt tctgtaaaaa
     1801 tgcagctcag attctttgtt tgaaaaatta gcgctctcgc gttgcatttt tgttctacaa
     1861 aatgaagcac agatgcttcg ttcaggtggc acttttcggg gaaatgtgcg cggaacccct
     1921 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     1981 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     2041 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     2101 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     2161 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     2221 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     2281 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     2341 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     2401 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     2461 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     2521 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     2581 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     2641 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     2701 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     2761 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     2821 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     2881 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     2941 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     3001 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3061 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3121 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3181 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3241 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3301 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3361 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3421 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3481 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3541 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3601 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3661 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3721 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     3781 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     3841 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     3901 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     3961 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     4021 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     4081 aagctggagc tctagtacgg attagaagcc gccgagcggg tgacagccct ccgaaggaag
     4141 actctcctcc gtgcgtcctc gtcttcaccg gtcgcgttcc tgaaacgcag atgtgcctcg
     4201 cgccgcactg ctccgaacaa taaagattct acaatactag cttttatggt tatgaagagg
     4261 aaaaattggc agtaacctgg ccccacaaac cttcaaatga acgaatcaaa ttaacaacca
     4321 taggatgata atgcgattag ttttttagcc ttatttctgg ggtaattaat cagcgaagcg
     4381 atgatttttg atctattaac agatatataa atgcaaaaac tgcataacca ctttaactaa
     4441 tactttcaac attttcggtt tgtattactt cttattcaaa tgtaataaaa gtatcaacaa
     4501 aaaattgtta atatacctct atactttaac gtcaaggaga aaaaaccccg gattctagaa
     4561 ctagtggatc ccccatcaca agtttgtaca aaaaagctga acgagaaacg taaaatgata
     4621 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
     4681 cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc
     4741 ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa
     4801 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     4861 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     4921 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     4981 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
     5041 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     5101 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     5161 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     5221 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     5281 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     5341 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     5401 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
     5461 cggggcgtaa acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg
     5521 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag
     5581 aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc
     5641 aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc
     5701 gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc
     5761 ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt
     5821 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
     5881 attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
     5941 gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
     6001 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
     6061 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg
     6121 tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt
     6181 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
     6241 atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatgggct gcaggaattc
     6301 gatatcaagc ttaatggtga gcgagctgat taaggagaac atgcacatga agctgtacat
     6361 ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta
     6421 cgagggcacc cagaccatga gaatcaaggc ggtcgagggc ggccctctcc ccttcgcctt
     6481 cgacatcctg gctaccagct tcatgtacgg cagcaaaacc ttcatcaacc acacccaggg
     6541 catccccgac ttctttaagc agtccttccc cgagggcttc acatgggaga gagtcaccac
     6601 atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct
     6661 catctacaac gtcaagatca gaggggtgaa cttcccatcc aacggccctg tgatgcagaa
     6721 gaaaacactc ggctgggagg cctccaccga gaccctgtac cccgctgacg gcggcctgga
     6781 aggcagagcc gacatggccc tgaagctcgt gggcgggggc cacctgatct gcaacttgaa
     6841 gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggcg tctactatgt
     6901 ggacagaaga ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg agcagcacga
     6961 ggtggctgtg gccagatact gcgacctccc tagcaaactg gggcacagat aactcgagtc
     7021 atgtaattag ttatgtcacg cttacattca cgccctcccc ccacatccgc tctaaccgaa
     7081 aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta
     7141 gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacag acgcgtgtac
     7201 gcatgtaaca ttatactgaa aaccttgctt gagaaggttt tgggacgctc gaaggcttta
     7261 atttgcggcc ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     7321 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     7381 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     7441 aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc
     7501 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     7561 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7621 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7681 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     7741 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     7801 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     7861 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     7921 aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataggg
     7981 taataactga tataattaaa ttgaagctct aatttgtgag tttagtatac atgcatttac
     8041 ttataataca gttttttagt tttgctggcc gcatcttctc aaatatgctt cccagcctgc
     8101 ttttctgtaa cgttcaccct ctaccttagc atcccttccc tttgcaaata gtcctcttcc
     8161 aacaataata atgtcagatc ctgtagagac cacatcatcc acggttctat actgttgacc
     8221 caatgcgtct cccttgtcat ctaaacccac accgggtgtc ataatcaacc aatcgtaacc
     8281 ttcatctctt ccacccatgt ctctttgagc aataaagccg ataacaaaat ctttgtcgct
     8341 cttcgcaatg tcaacagtac ccttagtata ttctccagta gatagggagc ccttgcatga
     8401 caattctgct aacatcaaaa ggcctctagg ttcctttgtt acttcttctg ccgcctgctt
     8461 caaaccgcta acaatacctg ggcccaccac accgtgtgca ttcgtaatgt ctgcccattc
     8521 tgctattctg tatacacccg cagagtactg caatttgact gtattaccaa tgtcagcaaa
     8581 ttttctgtct tcgaagagta aaaaattgta cttggcggat aatgccttta gcggcttaac
     8641 tgtgccctcc atggaaaaat cagtcaagat atccacatgt gtttttagta aacaaatttt
     8701 gggacctaat gcttcaacta actccagtaa ttccttggtg gtacgaacat ccaatgaagc
     8761 acacaagttt gtttgctttt cgtgcatgat attaaat
//
