LOCUS       sequence-25614-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Muehlich S, Wang R, Lee SM, Lewis TC, Dai C, Prywes R
  TITLE     Serum-induced phosphorylation of the serum response factor 
            coactivator MKL1 by the extracellular signal-regulated kinase 1/2 
            pathway inhibits its nuclear localization.
  JOURNAL   Mol Cell Biol. 2008 Oct;28(20):6302-13. doi: 10.1128/MCB.00427-08. 
            Epub 2008 Aug 11.
  PUBMED    18694962
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00427-08"; journalName: "Mol Cell Biol"; date: 
            "2008-10"; volume: "28"; issue: "20"; pages: "6302-13"
COMMENT     Sequence Label: pRevTRE MKL1-N100
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             119..184
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(239..250)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 acaaangagc atnngctntt ttgacctccn tagaagacac cgggaccgat ccagcctccg
       61 cggccccgaa ttcntngctc ggtacccggg gatccgtgaa ccgtcagaat taaccatgga
      121 ctacaaagac catgacggtg attataaaga tcatgacatc gattacaagg atgacgatga
      181 caagcttgcg gccgcgaatt cggtgggcca ggtgaactat cccaaagtag cagacagctc
      241 ttccttcgat gaggacagca gcgatgcctt atcccccgag cagcctgcca gccatgagtc
      301 ccagggttct gtgccgtcac ccctggaggc ccgagtcagc gaaccactgc tcagtgccac
      361 ctctgcatcc cccacccagg ttgtgtctca acttccgatg ggccgggatt ccagagaaat
      421 gcttttcctg gcagagcagc ctcctctgcc tcccccacct ctgctgcctc ccagcctcac
      481 caatggaacc actatcccca ctgccaagtc cacccccaca ctcattaagc aaagccaacc
      541 caagtctgnn gnggnnanan nnctcnncan caagaaaggc caaggagctg nagccnnagg
      601 tgaagaagct caagtaccac angtgcatcc ccccgganga gaagcaggac agggggcncc
      661 cccccatgng actcntccta cgcaagatct gganancngg agtnttcnct acagctgnan
      721 gtctcnacng cntagcagcg cacacantac caggcatctg ctgcccgcaa gtagagggag
      781 cctgggagag ctggaccccc agancacctt cntacaaaga ctncgcnggg ncctggctgg
      841 gtggcnnaaa gactntgtga acggactcgc acgaganagg aantaaga
//
