LOCUS       sequence-256142-        9821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9821)
  AUTHORS   Newby GA, Kiriakov S, Hallacli E, Kayatekin C, Tsvetkov P, Mancuso 
            CP, Bonner JM, Hesse WR, Chakrabortee S, Manogaran AL, Liebman SW, 
            Lindquist S, Khalil AS
  TITLE     A Genetic Tool to Track Protein Aggregates and Control Prion 
            Inheritance.
  JOURNAL   Cell. 2017 Nov 2;171(4):966-979.e18. doi: 
            10.1016/j.cell.2017.09.041. Epub 2017 Oct 19.
  PUBMED    29056345
REFERENCE   2  (bases 1 to 9821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.041"; journalName: "Cell"; date: "2017-11-2-
            2"; volume: "171"; issue: "4"; pages: "966-979.e18"
COMMENT     Sequence Label: pSK260
FEATURES             Location/Qualifiers
     source          1..9821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    77..93
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     82..104
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     101..117
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     101..117
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1634..1658
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2419..2443)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2481..2501
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3105..3194
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             3201..3290
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      4915..5102
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4970..4994)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            5177..6296
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        5177..5520
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5521..6093
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      6099..6296
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     6866..6882
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6866..6882
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6896..6915)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6897..6915)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6922..6939)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6922..6938)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6931..6953)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7238..7257)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7370..7391
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7545..7564)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7664..7686
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7724..7742)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7810..7914
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7915..7983,7984..8775)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8133..8152)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8946..9534
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9435..9454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9688..9705
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccgcggct
       61 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
      121 gattacgcca ctagtccgag gcctcgagat ccgatatcgc cgtggcggcc gccagctgaa
      181 gcttaattat cctgggcacg agtgaaacaa agctaaaacc tttatttagc atggccattg
      241 aatgtaacaa ttatatatat cgcaagcaca aaaaatcaag gagagagaac taccactttg
      301 ttcatgtgta caatgttcat tatctccata agcaaaaaaa aaaaaataga aaacatatgc
      361 tataaggttg atattctcac gagtaagcgg cacttgctac ttattgacat tgcagatttt
      421 tggctacaga aatagtatat tagagattat aattgctaat caaatcaaaa tataaaatta
      481 gtaaaccaaa ccatttatac ccttccttag tagttatgga ttgtttttta atgatatttc
      541 tgcaaaccaa agaaagattg ttatccagat agaatttagt tttgatattc atttttttgt
      601 tgaagattga acgccatatc tgggcctcat aattcaaaag acggtgccat tatcggtagc
      661 gtttcgcatt gtactggatt tcagaaattt cacagttgat gaatcgaaaa gaatggtctc
      721 attgcaacac gtaaggttaa gatgtccctt tttaccatta taggcaataa atgaatcata
      781 aaacgaccgt atactggtga aatagtaggg agaacgagta cctgtagtaa aaagtataaa
      841 tcatagttaa tcgggcaatg tccctcgatc aaggagtatt gtgtcatgtt cgagacaaac
      901 gccaacattt ttgtttcttt tggacaaatg ttgtttgcat ttatgatccg ttatattttg
      961 atctaatgta gagttgcacg tagttcttac tggcaaagaa atcgatgcat accaaaaaag
     1021 aataaaggtg atatttgatc tttaccgttt agttccaacg taaaattgtg cctttggact
     1081 taaaatggcg tcgtacgctg caggtcgacg gatcccaacc acacaaaaat catacaacga
     1141 atggtatatg cttcatttct ttgtttcgca ttagctgcgc tatttgactc aaattattat
     1201 tttttactaa gacgacgcgt cacagtgttc gagtctgtgt catttctttt gtaattctct
     1261 taaaccactt cataaagttg tgaagttcat agcaaaattc ttccgcaaaa agatgaatct
     1321 tagttctcag cccaccaaaa gaggtacatg ctaagatcat acagaagtta ttgtcacttc
     1381 ttaccttgct cttaaatgta cattacaacc gggtattata tcttacatca tcgtataata
     1441 tgatctttct ttatggagaa aatttttttt tcactcgacc aaagctccca ttgcttctga
     1501 agagtgtagt gtatattggt acatcttctc ttgaaagact ccattgtact gtaacaaaaa
     1561 gcggtttctt catcgacttg ctcggaataa catctatatc tgcccactag caacaactag
     1621 tggatccccc atcacaagtt tgtacaaaaa agcaggctat gtcggattca aaccaaggca
     1681 acaatcagca aaactaccag caatacagcc agaacggtaa ccaacaacaa ggtaacaaca
     1741 gataccaagg ttatcaagct tacaatgctc aagcccaacc tgcaggtggg tactaccaaa
     1801 attaccaagg ttattctggg taccaacaag gtggctatca acagtacaat cccgacgccg
     1861 gttaccagca acagtataat cctcaaggag gctatcaaca gtacaatcct caaggcggtt
     1921 atcagcagca attcaatcca caaggtggcc gtggaaatta caaaaacttc aactacaata
     1981 acaatttgca aggatatcaa gctggtttcc aaccacagtc tcaaggtatg tctttgaacg
     2041 actttcaaaa gcaacaaaag caggccgctc ccaaaccaaa gaagactttg aagcttgtct
     2101 ccagttccgg tatcaagttg gccaatgcta ccaagaaggt tggcacaaaa cctgccgaat
     2161 ctgataagaa agaggaagag aagtctgctg aaaccaaaga accaactaaa gagccaacaa
     2221 aggtcgaaga accagttaaa aaggaggaga aaccagtcca gactgaagaa aagacggagg
     2281 aaaaatcgga acttccaaag gtagaagacc ttaaaatctc tgaatcaaca cataatacca
     2341 acaatgccaa tgttaccagt gctgatgcct tgatcaagga acaggaagaa gaagtggatg
     2401 acgaagttgt taacgatgac ccggctttct tgtacaaagt ggtgatgggc tgcaggaatt
     2461 cgatatcaag cttaatggct ccaaagaaga agagaaaggt tggtattcat ggtgttccag
     2521 ttgatggtgg tttacaagct ccaccaactg atgtttcttt gggtgatgaa ttgcatttgg
     2581 atggtgaaga tgttgctatg gctcatgctg atgctttgga tgattttgat ttggatatgt
     2641 tgggtgacgg tgattctcca ggtccaggtt ttactccaca tgattctgct ccatatggtg
     2701 ctttagatat ggctgacttt gaattcgaac aaatgttcac cgatgccttg ggtattgatg
     2761 aatatggtgg tggtgctaga ccaggtgaaa gaccatttca atgtagaatc tgcatgagaa
     2821 acttctccag acaagataga ttggatagac ataccagaac tcatactggt gaaaagccat
     2881 tccaatgcag aatatgtatg agaaattttt cccaaaaaga acacttggcc ggtcatttga
     2941 gaacacacac aggtgaaaaa ccttttcaat gcagaatatg catgagaaac tttagtagaa
     3001 gagacaactt gaacagacac ttgaaaaccc atttgagagg ttctgccggc gaattggctt
     3061 tgaaattggc tggtttggat atcaattctg gttctgctgc tagataccca tatgatgttc
     3121 cagattatgc tggttaccct tacgatgttc ctgactacgc aggttcttat ccatacgatg
     3181 tacctgatta tgcctcaaga tatccttatg acgtccctga ttacgccggt tatccatatg
     3241 acgtaccaga ctacgctggt agttacccat acgacgtccc agattacgct tcttcataat
     3301 gactcgagtc atgtaatttc ttgcaaacat aagtaaatgc aaacacaata ataccgatca
     3361 taaagcattt tcttctatat taaaaaacaa ggtttaataa agctgttata tatatatata
     3421 tatatataga cgtataatta gtttagttct ttttgtacca tataccataa acaaggtaaa
     3481 cttcacctct caatatatct agaatttcat aaaaatatct agcaaggttt caactccttc
     3541 aatcacgttt tcatcactca accctatctc ggtctattct tttgatttat aagggatttt
     3601 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     3661 taacaaaata ttaacgttta caatttcctg atgcggtatt ttctttaatt aaagagtgag
     3721 gactcgaaaa tattaataga gtgaggactc gaaaatatta atagagtgag gactcgaaaa
     3781 tattaataga gtgaggactc gaaaatatta atatcgatag agtgaggact cgaaaatatt
     3841 aatagagtga ggactcgaaa atattaatga attcagagtg aggactcgaa aatattaata
     3901 gagtgaggac tcgaaaatat taatggccgg cccagatccg ccaggcgtgt atatatagcg
     3961 tggatggcca ggcaacttta gtgctgacac atacaggcat atatatatgt gtgcgacgac
     4021 acatgatcat atggcatgca tgtgctctgt atgtatataa aactcttgtt ttcttctttt
     4081 ctctaaatat tctttcctta tacattagga cctttgcagc ataaattact atacttctat
     4141 agacacacaa acacaaatac acacactgtt taaacaaaaa atgggttctg gtggttctga
     4201 attgatcaaa gaaaacatgc acatgaagtt gtacatggaa ggtactgtta acaaccatca
     4261 tttcaagtgt acctctgaag gtgaaggtaa accatatgaa ggtactcaaa ccatgagaat
     4321 caaagctgtt gaaggtggtc cattgccatt tgcttttgat attttggcta cctccttcat
     4381 gtacggttct aagactttca ttaaccacac ccaaggtatc ccagatttct tcaaacaatc
     4441 tttcccagaa ggtttcactt gggaaagagt tactacttac gaagatggtg gtgttttgac
     4501 tgctactcaa gatacttctt tacaagacgg ttgcttgatc tacaacgtta agattagagg
     4561 tgtcaacttc ccatctaatg gtccagttat gcaaaaaaag actttgggtt gggaagcttc
     4621 tactgaaaca ttatatccag ctgatggtgg tttggaaggt agagctgata tggctttgaa
     4681 attggttggt ggtggtcatt tgatctgcaa cttgaaaact acctacagat ctaagaagcc
     4741 agccaagaat ttgaaaatgc caggtgttta ctacgtcgat agaagattgg aaagaatcaa
     4801 agaagccgac aaagaaacct acgttgaaca acacgaagtt gctgttgcta gatattgtga
     4861 tttgccatcc aaattaggtc atagaggatc ttaaggcgcg ccacttctaa ataagcgaat
     4921 ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa
     4981 ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg
     5041 taggtcaggt tgctttctca ggtatagtat gaggtcgctc ttattgacca cacctctacc
     5101 ggcagatccg ctagggataa cagggtaata tagatctgtt tagcttgcct cgtccccgcc
     5161 gggtcacccg gccagcgaca tggaggccca gaataccctc cttgacagtc ttgacgtgcg
     5221 cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc cccatgtata
     5281 atcatttgca tccatacatt ttgatggccg cacggcgcga agcaaaaatt acggctcctc
     5341 gctgcagacc tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt gtccccacgc
     5401 cgcgcccctg tagagaaata taaaaggtta ggatttgcca ctgaggttct tctttcatat
     5461 acttcctttt aaaatcttgc taggatacag ttctcacatc acatccgaac ataaacaacc
     5521 atgggtacca ctcttgacga cacggcttac cggtaccgca ccagtgtccc gggggacgcc
     5581 gaggccatcg aggcactgga tgggtccttc accaccgaca ccgtcttccg cgtcaccgcc
     5641 accggggacg gcttcaccct gcgggaggtg ccggtggacc cgcccctgac caaggtgttc
     5701 cccgacgacg aatcggacga cgaatcggac gacggggagg acggcgaccc ggactcccgg
     5761 acgttcgtcg cgtacgggga cgacggcgac ctggcgggct tcgtggtcgt ctcgtactcc
     5821 ggctggaacc gccggctgac cgtcgaggac atcgaggtcg ccccggagca ccgggggcac
     5881 ggggtcgggc gcgcgttgat ggggctcgcg acggagttcg cccgcgagcg gggcgccggg
     5941 cacctctggc tggaggtcac caacgtcaac gcaccggcga tccacgcgta ccggcggatg
     6001 gggttcaccc tctgcggcct ggacaccgcc ctgtacgacg gcaccgcctc ggacggcgag
     6061 caggcgctct acatgagcat gccctgcccc taatcagtac tgacaataaa aagattcttg
     6121 ttttcaagaa cttgtcattt gtatagtttt tttatattgt agttgttcta ttttaatcaa
     6181 atgttagcgt gatttatatt ttttttcgcc tcgacatcat ctgcccagat gcgaagttaa
     6241 gtgcgcagaa agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct atactgctgt
     6301 cgattcgata ctaacgccgc catccagtgt cgaaaacgag ctcgaattcc tgggggaaca
     6361 acttcacaga atgttttgtc atattgtcga agtggtcaca aaacaagaga agttccgcca
     6421 attataaaaa gggaacccgt atatttcagc ttcacggatg atttccaggg tgagagtact
     6481 gtatatgggc ttacgataga aggccataaa aatttcttgc ttggcaacaa aatagaagtg
     6541 aaatcatgtc gaggctgctg tgtgggagaa cagcataaaa tatcacaaaa aaagaatcta
     6601 aaacactgtg ttgcttgtcc cagaaaggga atcaagtatt tttataaaga ttggagtggt
     6661 aaaaatcgag tatgtgctag atgctatgga agatacaaat tcagcggtca tcactgtata
     6721 aattgcaagt atgtaccaga agcacgtgaa gtgaaaaagg caaaagacaa aggcgaaaaa
     6781 ttgggcatta cgcccgaagg tttgccagtt aaaggaccag agtgtataaa atgtggcgga
     6841 atcttacagt ggcctatgcg gccgctctag aactagtgga tcgatcccca attcgcccta
     6901 tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     6961 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     7021 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     7081 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
     7141 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     7201 agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc
     7261 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggggtgg gccatcgccc
     7321 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7381 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
     7441 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7501 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
     7561 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     7621 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     7681 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     7741 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     7801 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7861 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7921 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7981 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     8041 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     8101 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     8161 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     8221 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8281 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8341 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8401 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     8461 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8521 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8581 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8641 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8701 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8761 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8821 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8881 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8941 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     9001 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     9061 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     9121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     9181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     9241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     9301 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     9361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     9421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     9481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     9541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     9601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     9661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     9721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     9781 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa t
//
