LOCUS       sequence-256145-        4693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes two fluorecent proteins with RNA binding peptides: 
            mTurquoise2-Tat and YPet-PCP. Expression with constitutive E. coli 
            RNAP promoter (J23106), ribozymes RiboJ10 (for YPet) and PlmJ (for 
            mTq2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Schwarz-Schilling M, Dupin A, Chizzolini F, Krishnan S, Mansy SS, 
            Simmel FC
  TITLE     Optimized Assembly of a Multifunctional RNA-Protein Nanostructure in
            a Cell-Free Gene Expression System.
  JOURNAL   Nano Lett. 2018 Apr 11;18(4):2650-2657. doi: 
            10.1021/acs.nanolett.8b00526. Epub 2018 Mar 27.
  PUBMED    29564885
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.nanolett.8b00526"; journalName: "Nano Lett"; date: 
            "2018-04-11- 11"; volume: "18"; issue: "4"; pages: "2650-2657"
COMMENT     Sequence Label: pSB1C3_mTq2-Tat_YPet-PCP (cy-plasmid)
FEATURES             Location/Qualifiers
     source          1..4693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     572..591
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     819..837
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(841..884)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     RBS             1815..1826
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(1923..1957)
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     promoter        2214..2248
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             2337..2348
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    3532..3552
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      3553..3610
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(3634..3651)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3805..4393)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3885..3904)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4576..4670)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(4691..4693,1..657))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
       61 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
      121 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
      181 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc
      241 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
      301 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
      361 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
      421 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
      481 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
      541 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
      601 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
      661 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
      721 ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg
      781 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga
      841 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgagtaagc
      901 cgcttctaga gcaccgattg agaagagaaa agaaaaccgc cgatcctgtc caccgcatta
      961 ctgcaaggta gtggacaaga ccggcggtct taagtttttt ggctgaaagt agtaccatta
     1021 acgacgaatg cgacgaccct taccgcgggt accacgagga cgcggaccgc tgctgccgcc
     1081 gccaccacgg gtcttgtaca gttcgtccat acccagcgtg atacccgccg cggtaacaaa
     1141 ttccagcagg accatgtggt cacgtttttc gttcggatct ttggacagtt tggattgcgt
     1201 gctcaagtag tgattatctg gcagcaggac cggaccatcg ccaatcgggg tgttctgttg
     1261 atagtgatcg gccaattgca cgccgccatc ttcaatattg tgacggatct tgaagtttgc
     1321 tttaatgccg tttttctgtt tatcagcggt gatgtacaca ttgtcgctga agtaattata
     1381 ctccagtttg tgacccagga tgttaccgtc ctctttaaag tcaatacctt tcagctcaat
     1441 acgattcacc agcgtgtcac cctcgaactt gacttctgcg cgggtcttat agttaccgtc
     1501 gtctttgaaa aagatcgtac gttcctgaac gtagccttcc ggcatcgcgc tcttaaagaa
     1561 gtcatgctgt ttcatgtgat ccggatagcg cgcgaaacat tgcacgcccc acgacagagt
     1621 cgtcaccagg gtcggccacg gaaccggcag cttaccggtc gtgcagataa acttcagggt
     1681 caatttaccg taggtggcgt cgccctcgcc ctcaccgcta acgctaaact tgtgaccgtt
     1741 gacatcacca tccagctcaa ccagaatcgg cacgacgccg gtgaacagtt cttcaccctt
     1801 gctaaccatg tctatttctc ctctttactg actttaaaca aaattatttg tagaggtcat
     1861 aagtttcgtc gcatttcagc gactcatcag tgggcttagc ccagacttat gactgaagct
     1921 tcgctagcac tatacctagg actgagctag ccgtaaagat cctgaatctc gagattggtg
     1981 ccttgcttgg attatgaaat gccagcctga tcatacaacg ttggacgaag tagctttacc
     2041 gtccacacgg tcggaacttg gtgtgccgaa tccaagtcga gataatcact agatgtcctg
     2101 aggtgcatac tagaggacca cgcatcgtga tgcctatgcg cggtagtccc accttgtcca
     2161 ctagaatgga agattggcac gtatcaagac tttggagtag taccataacg ccgtttacgg
     2221 ctagctcagt cctaggtata gtgctagcta gcgcagcgct caacgggtgt gcttcccgtt
     2281 ctgatgagtc cgtgaggacg aaagcgcctc tacaaataat tttgtttaat catgagaaag
     2341 aggagaaaac tagatgtcta aaggtgaaga actgtttacg ggtgtcgtgc cgattctggt
     2401 cgagttggac ggcgacgtga acggtcacaa attcagcgtg agcggcgagg gcgagggtga
     2461 cgcgacgtac ggtaagctga ctctgaagct gctgtgcacc acgggtaaat tgccggttcc
     2521 gtggccgacc ctggtcacga cgctgggtta tggtgtacaa tgttttgcac gctatccgga
     2581 ccacatgaaa cagcacgatt tcttcaagag cgcgatgccg gaaggctatg ttcaggaacg
     2641 taccatcttt ttcaaagatg atggtaatta caaaacccgc gcagaagtga agttcgaggg
     2701 tgacaccctg gtgaaccgta ttgagctgaa gggtattgac ttcaaggaag atggcaatat
     2761 tctgggtcac aaactggagt acaactataa cagccataac gtctacatca ccgcggataa
     2821 gcaaaaaaat ggtatcaaag caaatttcaa gattcgccac aacatcgaag atggcggcgt
     2881 gcaactggcc gatcattatc agcagaatac cccaatcggt gacggtccgg tgctgttgcc
     2941 ggataaccac tacctgagct atcaaagcgc gttgttcaaa gacccgaatg aaaaacgtga
     3001 ccacatggtt ctgctggaat ttctgaccgc tgcgggcatc actgaaggca tgaatgaact
     3061 gtacaagacg cgtggtggcg gcggttcgat gagcaagact atcgttttgt ccgtcggcga
     3121 ggctacccgt accttgaccg aaattcaatc caccgcggac cgtcaaattt ttgaggaaaa
     3181 agtcggtcct ctggtgggtc gtctgcgtct gaccgcgagc ctgcgccaga acggtgccaa
     3241 aacggcatac cgtgttaatc tgaaactgga tcaggccgac gttgtggaca gcggtctgcc
     3301 gaaagtccgc tacacccagg tgtggagcca cgatgtgacg atcgttgcga atagcaccga
     3361 agcgagccgc aagagcctgt acgacctgac caagagcctg gtggcaacgt cccaagttga
     3421 agatctggtt gttaacctgg tgccgctggg tcgttaaagc atgccggagg aaacacagaa
     3481 aaaagcccgc acctgacagt gcgggctttt tttttcgacc aaaggggtgc atactagtag
     3541 cggccgctgc agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa
     3601 accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt
     3661 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3721 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3781 taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa
     3841 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3901 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3961 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4021 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4081 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4141 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4201 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     4261 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4321 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4381 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4441 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     4501 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     4561 cagctcgagg cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     4621 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     4681 cgatatcaaa tta
//
