LOCUS       sequence-256166-       10251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yTRAP sensor of [RNQ+], detects aggregation of Rnq1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10251)
  AUTHORS   Newby GA, Kiriakov S, Hallacli E, Kayatekin C, Tsvetkov P, Mancuso 
            CP, Bonner JM, Hesse WR, Chakrabortee S, Manogaran AL, Liebman SW, 
            Lindquist S, Khalil AS
  TITLE     A Genetic Tool to Track Protein Aggregates and Control Prion 
            Inheritance.
  JOURNAL   Cell. 2017 Nov 2;171(4):966-979.e18. doi: 
            10.1016/j.cell.2017.09.041. Epub 2017 Oct 19.
  PUBMED    29056345
REFERENCE   2  (bases 1 to 10251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.041"; journalName: "Cell"; date: "2017-11-2-
            2"; volume: "171"; issue: "4"; pages: "966-979.e18"
COMMENT     Sequence Label: pGAN201
FEATURES             Location/Qualifiers
     source          1..10251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..17
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6..28
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     25..41
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     25..41
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1534..1558
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2780..2804)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2842..2862
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3466..3555
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             3562..3651
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     regulatory      4788..4797
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      5264..5451
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5319..5343)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            5526..6657
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     CDS             5876..6448
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      6460..6657
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     7220..7236
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7220..7236
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7250..7269)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7251..7269)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7276..7293)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7276..7292)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7285..7307)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7592..7611)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7724..7745
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7899..7918)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8018..8040
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8078..8096)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8164..8268
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8269..8337,8338..9129)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8487..8506)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9300..9888
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9789..9808
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10042..10059
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10176..10197
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccactagtc
       61 cgaggcctcg agatccgata tcgccgtggc ggccgcaatt atcctgggca cgagtgaaac
      121 aaagctaaaa cctttattta gcatggccat tgaatgtaac aattatatat atcgcaagca
      181 caaaaaatca aggagagaga actaccactt tgttcatgtg tacaatgttc attatctcca
      241 taagcaaaaa aaaaaaaata gaaaacatat gctataaggt tgatattctc acgagtaagc
      301 ggcacttgct acttattgac attgcagatt tttggctaca gaaatagtat attagagatt
      361 ataattgcta atcaaatcaa aatataaaat tagtaaacca aaccatttat acccttcctt
      421 agtagttatg gattgttttt taatgatatt tctgcaaacc aaagaaagat tgttatccag
      481 atagaattta gttttgatat tcattttttt gttgaagatt gaacgccata tctgggcctc
      541 ataattcaaa agacggtgcc attatcggta gcgtttcgca ttgtactgga tttcagaaat
      601 ttcacagttg atgaatcgaa aagaatggtc tcattgcaac acgtaaggtt aagatgtccc
      661 tttttaccat tataggcaat aaatgaatca taaaacgacc gtatactggt gaaatagtag
      721 ggagaacgag tacctgtagt aaaaagtata aatcatagtt aatcgggcaa tgtccctcga
      781 tcaaggagta ttgtgtcatg ttcgagacaa acgccaacat ttttgtttct tttggacaaa
      841 tgttgtttgc atttatgatc cgttatattt tgatctaatg tagagttgca cgtagttctt
      901 actggcaaag aaatcgatgc ataccaaaaa agaataaagg tgatatttga tctttaccgt
      961 ttagttccaa cgtaaaattg tgcctttgga cttaaaatgg cgtcgtacgc tgcaccgagt
     1021 gcaaccacac aaaaatcata caacgaatgg tatatgcttc atttctttgt ttcgcattag
     1081 ctgcgctatt tgactcaaat tattattttt tactaagacg acgcgtcaca gtgttcgagt
     1141 ctgtgtcatt tcttttgtaa ttctcttaaa ccacttcata aagttgtgaa gttcatagca
     1201 aaattcttcc gcaaaaagat gaatcttagt tctcagccca ccaaaagagg tacatgctaa
     1261 gatcatacag aagttattgt cacttcttac cttgctctta aatgtacatt acaaccgggt
     1321 attatatctt acatcatcgt ataatatgat ctttctttat ggagaaaatt tttttttcac
     1381 tcgaccaaag ctcccattgc ttctgaagag tgtagtgtat attggtacat cttctcttga
     1441 aagactccat tgtactgtaa caaaaagcgg tttcttcatc gacttgctcg gaataacatc
     1501 tatatctgcc cactagcaac agctagcccc atcacaagtt tgtacaaaaa agcaggctac
     1561 aaaatggata cggataagtt aatctcagag gctgagtctc atttttctca aggaaaccat
     1621 gcagaagctg ttgcgaagtt gacatccgca gctcagtcga accccaatga cgagcaaatg
     1681 tcaactattg aatcattaat tcaaaaaatc gcaggatacg tcatggacaa ccgtagtggt
     1741 ggtagtgacg cctcgcaaga tcgtgctgct ggtggtggtt catcttttat gaacacttta
     1801 atggcagact ctaagggttc ttcccaaacg caactaggaa aactagcttt gttagccaca
     1861 gtgatgacac actcatcaaa taaaggttct tctaacagag ggtttgacgt agggactgtc
     1921 atgtcaatgc taagtggttc tggcggcggg agccaaagta tgggtgcttc cggcctggct
     1981 gccttggctt ctcaattctt taagtcaggt aacaattccc aaggtcaggg acaaggtcaa
     2041 ggtcaaggtc aaggtcaagg acaaggtcaa ggtcaaggtt cttttactgc tttggcgtct
     2101 ttggcttcat ctttcatgaa ttccaacaac aataatcagc aaggtcaaaa tcaaagctcc
     2161 ggtggttcct cctttggagc actggcttct atggcaagct cttttatgca ttccaataat
     2221 aatcagaact ccaacaatag tcaacagggc tataaccaat cctatcaaaa cggtaaccaa
     2281 aatagtcaag gttacaataa tcaacagtac caaggtggca acggtggtta ccaacaacaa
     2341 cagggacaat ctggtggtgc tttttcctca ttggcctcca tggctcaatc ttacttaggt
     2401 ggtggacaaa ctcaatccaa ccaacagcaa tacaatcaac aaggccaaaa caaccagcag
     2461 caataccagc aacaaggcca aaactatcag catcaacaac agggtcagca gcagcaacaa
     2521 ggccactcca gttcattctc agctttggct tccatggcaa gttcctacct gggcaataac
     2581 tccaattcaa attcgagtta tgggggccag caacaggcta atgagtatgg tagaccgcaa
     2641 cagaatggtc aacagcaatc caatgagtac ggaagaccgc aatacggcgg aaaccagaac
     2701 tccaatggac agcacgaatc cttcaatttt tctggcaact tttctcaaca gaacaataac
     2761 ggcaaccaga accgctacaa cccagctttc ttgtacaaag tggtgatggg ctgcaggaat
     2821 tcgatatcaa gcttaatggc tccaaagaag aagagaaagg ttggtattca tggtgttcca
     2881 gttgatggtg gtttacaagc tccaccaact gatgtttctt tgggtgatga attgcatttg
     2941 gatggtgaag atgttgctat ggctcatgct gatgctttgg atgattttga tttggatatg
     3001 ttgggtgacg gtgattctcc aggtccaggt tttactccac atgattctgc tccatatggt
     3061 gctttagata tggctgactt tgaattcgaa caaatgttca ccgatgcctt gggtattgat
     3121 gaatatggtg gtggtgctag accaggtgaa agaccatttc aatgtagaat ctgcatgaga
     3181 aacttctcca gacaagatag attggataga cataccagaa ctcatactgg tgaaaagcca
     3241 ttccaatgca gaatatgtat gagaaatttt tcccaaaaag aacacttggc cggtcatttg
     3301 agaacacaca caggtgaaaa accttttcaa tgcagaatat gcatgagaaa ctttagtaga
     3361 agagacaact tgaacagaca cttgaaaacc catttgagag gttctgccgg cgaattggct
     3421 ttgaaattgg ctggtttgga tatcaattct ggttctgctg ctagataccc atatgatgtt
     3481 ccagattatg ctggttaccc ttacgatgtt cctgactacg caggttctta tccatacgat
     3541 gtacctgatt atgcctcaag atatccttat gacgtccctg attacgccgg ttatccatat
     3601 gacgtaccag actacgctgg tagttaccca tacgacgtcc cagattacgc ttcttcataa
     3661 tgagggccct catgtaattt cttgcaaaca taagtaaatg caaacacaat aataccgatc
     3721 ataaagcatt ttcttctata ttaaaaaaca aggtttaata aagctgttat atatatatat
     3781 atatatatag acgtataatt agtttagttc tttttgtacc atataccata aacaaggtaa
     3841 acttcacctc tcaatatatc tagaatttca taaaaatatc tagcaaggtt tcaactcctt
     3901 caatcacgtt ttcatcactc aaccctatct cggtctattc ttttgattta taagggattt
     3961 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4021 ttaacaaaat attaacgttt acaatttcct gatgcggtat tttctttaat taaagagtga
     4081 ggactcgaaa atattaatag agtgaggact cgaaaatatt aatagagtga ggactcgaaa
     4141 atattaatag agtgaggact cgaaaatatt aatatcgata gagtgaggac tcgaaaatat
     4201 taatagagtg aggactcgaa aatattaatg aattcagagt gaggactcga aaatattaat
     4261 agagtgagga ctcgaaaata ttaatggccg gcccagatcc gccaggcgtg tatatatagc
     4321 gtggatggcc aggcaacttt agtgctgaca catacaggca tatatatatg tgtgcgacga
     4381 cacatgatca tatggcatgc atgtgctctg tatgtatata aaactcttgt tttcttcttt
     4441 tctctaaata ttctttcctt atacattagg acctttgcag cataaattac tatacttcta
     4501 tagacacaca aacacaaata cacacactgt ttaaacaaaa aatgggcgag gaggataaca
     4561 tggcctctct cccagcgaca catgagttac acatctttgg ctccatcaac ggtgtggact
     4621 ttgacatggt gggtcagggc accggcaatc caaatgatgg ttatgaggag ttaaacctga
     4681 agtccaccaa gggtgacctc cagttctccc cctggattct ggtccctcat atcgggtatg
     4741 gcttccatca gtacctgccc taccctgacg ggatgtcgcc tttccaggcc gccatggtag
     4801 atggctccgg ataccaagtc catcgcacaa tgcagtttga agatggtgcc tcccttactg
     4861 ttaactaccg ctacacctac gagggaagcc acatcaaagg agaggcccag gtgaagggga
     4921 ctggtttccc tgctgacggt cctgtgatga ccaactcgct gaccgctgcg gactggtgca
     4981 ggtcgaagaa gacttacccc aacgacaaaa ccatcatcag tacctttaag tggagttaca
     5041 ccactggaaa tggcaagcgc taccggagca ctgcgcggac cacctacacc tttgccaagc
     5101 caatggcggc taactatctg aagaaccagc cgatgtacgt gttccgtaag acggagctca
     5161 agcactccaa gaccgagctc aacttcaagg agtggcaaaa ggcctttacc gatgtgatgg
     5221 gcatggacga gctgtacaag taaggcgcgc cacttctaaa taagcgaatt tcttatgatt
     5281 tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga
     5341 ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt aggtcaggtt
     5401 gctttctcag gtatagtatg aggtcgctct tattgaccac acctctaccg gcagatccgc
     5461 tagggataac agggtaatat agatctgttt agcttgcctc gtccccgccg ggtcacccgg
     5521 ccagcgacat ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg
     5581 catgatgtga ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat
     5641 ccatacattt tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct
     5701 gcgagcaggg aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt
     5761 agagaaatat aaaaggttag gatttgccac tgaggttctt ctttcatata cttcctttta
     5821 aaatcttgct aggatacagt tctcacatca catccgaaca tcaattgaaa caaccatggg
     5881 taccactctt gacgacacgg cttaccggta ccgcaccagt gtcccggggg acgccgaggc
     5941 catcgaggca ctggatgggt ccttcaccac cgacaccgtc ttccgcgtca ccgccaccgg
     6001 ggacggcttc accctgcggg aggtgccggt ggacccgccc ctgaccaagg tgttccccga
     6061 cgacgaatcg gacgacgaat cggacgacgg ggaggacggc gacccggact cccggacgtt
     6121 cgtcgcgtac ggggacgacg gcgacctggc gggcttcgtg gtcgtctcgt actccggctg
     6181 gaaccgccgg ctgaccgtcg aggacatcga ggtcgccccg gagcaccggg ggcacggggt
     6241 cgggcgcgcg ttgatggggc tcgcgacgga gttcgcccgc gagcggggcg ccgggcacct
     6301 ctggctggag gtcaccaacg tcaacgcacc ggcgatccac gcgtaccggc ggatggggtt
     6361 caccctctgc ggcctggaca ccgccctgta cgacggcacc gcctcggacg gcgagcaggc
     6421 gctctacatg agcatgccct gcccctaaag atcttcagta ctgacaataa aaagattctt
     6481 gttttcaaga acttgtcatt tgtatagttt ttttatattg tagttgttct attttaatca
     6541 aatgttagcg tgatttatat tttttttcgc ctcgacatca tctgcccaga tgcgaagtta
     6601 agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa tgctggtcgc tatactgctg
     6661 tcgattcgat actaacgccg ccatccagtg tcgaaaacga gctcgaattc ctgggggaac
     6721 aacttcacag aatgttttgt catattgtcg aagtggtcac aaaacaagag aagttccgcc
     6781 aattataaaa agggaacccg tatatttcag cttcacggat gatttccagg gtgagagtac
     6841 tgtatatggg cttacgatag aaggccataa aaatttcttg cttggcaaca aaatagaagt
     6901 gaaatcatgt cgaggctgct gtgtgggaga acagcataaa atatcacaaa aaaagaatct
     6961 aaaacactgt gttgcttgtc ccagaaaggg aatcaagtat ttttataaag attggagtgg
     7021 taaaaatcga gtatgtgcta gatgctatgg aagatacaaa ttcagcggtc atcactgtat
     7081 aaattgcaag tatgtaccag aagcacgtga agtgaaaaag gcaaaagaca aaggcgaaaa
     7141 attgggcatt acgcccgaag gtttgccagt taaaggacca gagtgtataa aatgtggcgg
     7201 aatcttacag tgcggccgct ctagaactag tggatcgatc cccaattcgc cctatagtga
     7261 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     7321 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     7381 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     7441 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     7501 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     7561 accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc
     7621 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gtgggccatc gccctgatag
     7681 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     7741 actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg
     7801 atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     7861 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
     7921 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     7981 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     8041 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     8101 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     8161 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     8221 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     8281 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     8341 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     8401 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     8461 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     8521 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     8581 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     8641 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     8701 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     8761 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     8821 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     8881 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     8941 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     9001 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     9061 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     9121 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     9181 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     9241 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     9301 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     9361 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     9421 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     9481 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     9541 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     9601 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     9661 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     9721 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     9781 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     9841 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     9901 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     9961 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    10021 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
    10081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
    10141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
    10201 gcaccccagg ctttacactt tatgcttccg cggctcgtat gttgtgtgga a
//
