LOCUS       sequence-25617-e         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Muehlich S, Wang R, Lee SM, Lewis TC, Dai C, Prywes R
  TITLE     Serum-induced phosphorylation of the serum response factor 
            coactivator MKL1 by the extracellular signal-regulated kinase 1/2 
            pathway inhibits its nuclear localization.
  JOURNAL   Mol Cell Biol. 2008 Oct;28(20):6302-13. doi: 10.1128/MCB.00427-08. 
            Epub 2008 Aug 11.
  PUBMED    18694962
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00427-08"; journalName: "Mol Cell Biol"; date: 
            "2008-10"; volume: "28"; issue: "20"; pages: "6302-13"
COMMENT     Sequence Label: pRevTRE MKL1-N100 S449A/T450A/S454A
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             131..196
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(251..262)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gtcagatcnc ctggagacgc catccacgct gttttgnncn nnnnnnaana caccgggacc
       61 gatccagcct ccgcggcccc gaattcgagc tcggtacccg gggatccgtg aaccgtcaga
      121 attaaccatg gactacaaag accatgacgg tgattataaa gatcatgaca tcgattacaa
      181 ggatgacgat gacaagcttg cggccgcgaa ttcggtgggc caggtgaact atcccaaagt
      241 ancagacagc tcttccttcg atgaggacag cagcgatgcc ttatcccccg agcagcctgc
      301 cagccatgag tcccagggtt ctgtgccgtc ncccctggag gcccgagtca gcgaaccact
      361 gctcagtgcc acctctgcat cccccaccca ggttgtgtct caacttccga tgggccggga
      421 ttccagagaa atgcttttcc tggcagagca gcctcctctg nnnnncccac ctctgctgcc
      481 tcccagcctc ancaatggaa ccactatccc cactgccaag tccacccccn cactcattaa
      541 gcaaagccaa ccnnnntctg ccantgagaa gtcacagcgc ancnnnaang ccanggagct
      601 gaaannnnna ggtgaanaat ctcaagtacc accnntnnnt ccnccccgga ncaganncnn
      661 gacagggggg nnncccccna tgnantcatc cnnacnccan nnncctgcan nancngcagc
      721 ttc
//
