LOCUS       sequence-256199-        8759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral constitutive expression of C20orf24 under control of its 
            native 3'UTR of human C20orf24 with variant from COX deficiency 
            patient..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8759)
  AUTHORS   Moutaoufik MT, Malty R, Amin S, Zhang Q, Phanse S, Gagarinova A, 
            Zilocchi M, Hoell L, Minic Z, Gagarinova M, Aoki H, Stockwell J, 
            Jessulat M, Goebels F, Broderick K, Scott NE, Vlasblom J, Musso G, 
            Prasad B, Lamantea E, Garavaglia B, Rajput A, Murayama K, Okazaki Y,
            Foster LJ, Bader GD, Cayabyab FS, Babu M
  TITLE     Rewiring of the Human Mitochondrial Interactome during Neuronal 
            Reprogramming Reveals Regulators of the Respirasome and 
            Neurogenesis.
  JOURNAL   iScience. 2019 Sep 4;19:1114-1132. doi: 10.1016/j.isci.2019.08.057.
  PUBMED    31536960
REFERENCE   2  (bases 1 to 8759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2019-09-4"; volume: "19:1114-1132. doi"; pages: " 
            10.1016/j.isci.2019.08.057"
COMMENT     Sequence Label: pPHAGE-C20orf24- C20orf24-3'UTR-VAR
FEATURES             Location/Qualifiers
     source          1..8759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     206..225
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     459..476
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    716..737
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(752..757,758..775,776..782)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    790..806
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     795..817
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     814..830
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     814..830
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     880..899
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1091..1724
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1771..1896
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2393..2626
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2811..2855
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3152..3269
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3321..3624
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3625..3828
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3779..3799
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3825..3849
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     3924..3943
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3924..3942
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3972..3996
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4408..4432)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4464..4487
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4503..4529
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             4503..4529
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    5042..5609
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5059..5611
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5203..5220)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5430..5449
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5623..6222
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5623..5642)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6119..6139
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6239..6827
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6292..6312)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6710..6721)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6902..7135
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7254..7272)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7340..7444
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7445..7513,7514..8305)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7663..7682)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8476..8759,1..305)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
       61 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      121 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      181 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      241 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      301 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      361 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      421 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      481 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      541 gcaagctcat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
      601 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
      661 ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
      721 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
      781 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga
      841 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
      901 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
      961 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
     1021 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     1081 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
     1141 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
     1201 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
     1261 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
     1321 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
     1381 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
     1441 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
     1501 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
     1561 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     1621 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     1681 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     1741 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     1801 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     1861 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     1921 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     1981 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     2041 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     2101 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     2161 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     2221 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     2281 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     2341 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     2401 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     2461 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     2521 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     2581 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     2641 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     2701 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     2761 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     2821 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     2881 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     2941 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     3001 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     3061 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa
     3121 ttaattcaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     3181 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     3241 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     3301 tttggactag tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     3361 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     3421 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     3481 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     3541 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     3601 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     3661 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     3721 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     3781 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     3841 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg
     3901 ccgcagatct ctcgaggttg atctaatacg actcactata gggagaccca agctggctag
     3961 ttaagctatc aacaagtttg tacaaaaaag ttggcatgag cggcgggcgg cggaaggagg
     4021 agccgcctca gccgcagctg gccaacgggg ccctcaaagt ctccgtctgg agtaaggtgc
     4081 tgcggagcga cgcggcctgg gaggataagg atgaattttt agatgtgatc tactggttcc
     4141 gacagatcat tgctgtggtc ctgggtgtca tttggggagt tttgccatta cgagggttct
     4201 tgggaatagc aggattctgc ctgatcaatg caggagtcct gtacctctac ttcagcaatt
     4261 acctacagat tgatgaggaa gaatatggtg gcacgtggga gctcacgaag gaagggttta
     4321 tgacctcttt tgccttgttc atggtatgtg tagctgatag ttttacaaca ggtcatttgg
     4381 atcatctttt acactgccat ctattatgcc caactttctt gtacaaagtg gttgatctag
     4441 agggcccgcg gttcgaactc gaggactaca aggacgacga tgacaagtcg gccgctggag
     4501 gataccccta cgacgtgccc gactacgcct agtggtgtac agctcccaag tgctccctat
     4561 ccagtccaaa ggaccctctt gattacagca caggaacttg atcgttgggg aaccccagcc
     4621 ccttggaact tggaagaccc gtgtttcctg gaccgcgaat cagtgtgttg ggcatcagtg
     4681 ttttctgcaa gggttgtgac ctgaaacttt ttaaaaacca cccacctttg gggaagcatt
     4741 tctgaattta tccatcacca accatttctt cttggatacc atcaagtaac agctattatt
     4801 tgccaagtgg agctgtcatt taatttgatg cacctctgga ttcagatgaa acattaaatt
     4861 gtcttcctcg attctccatc gggtgtagag tttttaaact atcaatggca tttcaagtct
     4921 tctgaaacaa catggctgta tgtgcgtggt ccatagcaca gtacatgcag catctaataa
     4981 gagtttccat tgtagaatgt tttcacatac ttgaataaat caaatcttta attgagaaaa
     5041 ttccgccccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     5101 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     5161 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     5221 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     5281 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     5341 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     5401 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     5461 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     5521 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     5581 tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatgaccga gtacaagccc
     5641 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     5701 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     5761 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     5821 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     5881 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     5941 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     6001 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     6061 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     6121 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     6181 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gatgcatcga tggatcctaa
     6241 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6301 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6361 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6421 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6481 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6541 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6601 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     6661 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6721 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6781 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta
     6841 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6901 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     6961 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7021 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7081 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     7141 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     7201 gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt
     7261 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     7321 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7381 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7441 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7501 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7561 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7621 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7681 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7741 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7801 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7861 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7921 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7981 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     8041 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8101 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8161 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8221 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8281 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8341 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8401 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8461 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8521 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8581 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8641 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8701 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagac
//
