LOCUS       sequence-256206-        8358 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral constitutive expression of CRISPR-resistant NENF with 
            c-terminal FLAG and HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8358)
  AUTHORS   Moutaoufik MT, Malty R, Amin S, Zhang Q, Phanse S, Gagarinova A, 
            Zilocchi M, Hoell L, Minic Z, Gagarinova M, Aoki H, Stockwell J, 
            Jessulat M, Goebels F, Broderick K, Scott NE, Vlasblom J, Musso G, 
            Prasad B, Lamantea E, Garavaglia B, Rajput A, Murayama K, Okazaki Y,
            Foster LJ, Bader GD, Cayabyab FS, Babu M
  TITLE     Rewiring of the Human Mitochondrial Interactome during Neuronal 
            Reprogramming Reveals Regulators of the Respirasome and 
            Neurogenesis.
  JOURNAL   iScience. 2019 Sep 4;19:1114-1132. doi: 10.1016/j.isci.2019.08.057.
  PUBMED    31536960
REFERENCE   2  (bases 1 to 8358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2019-09-4"; volume: "19:1114-1132. doi"; pages: " 
            10.1016/j.isci.2019.08.057"
COMMENT     Sequence Label: pPHAGE-CR-NENF-C-TAP
FEATURES             Location/Qualifiers
     source          1..8358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     206..225
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     459..476
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    716..737
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(752..757,758..775,776..782)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    790..806
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     795..817
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     814..830
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     814..830
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     880..899
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1091..1724
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1771..1896
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2393..2626
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2811..2855
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3152..3269
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3321..3624
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3625..3828
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3779..3799
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3825..3849
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     3924..3943
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3924..3942
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3972..3996
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4513..4537)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4569..4592
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4608..4634
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             4608..4634
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    4641..5208
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4658..5210
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4802..4819)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5029..5048
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5222..5821
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5222..5241)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5718..5738
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5838..6426
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5891..5911)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6309..6320)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6501..6734
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6853..6871)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6939..7043
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7044..7112,7113..7904)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7262..7281)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8075..8358,1..305)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
       61 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      121 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      181 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      241 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      301 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      361 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      421 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      481 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      541 gcaagctcat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
      601 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
      661 ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
      721 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
      781 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga
      841 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
      901 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
      961 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
     1021 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     1081 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
     1141 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
     1201 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
     1261 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
     1321 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
     1381 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
     1441 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
     1501 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
     1561 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     1621 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     1681 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     1741 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     1801 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     1861 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     1921 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     1981 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     2041 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     2101 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     2161 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     2221 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     2281 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     2341 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     2401 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     2461 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     2521 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     2581 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     2641 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     2701 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     2761 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     2821 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     2881 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     2941 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     3001 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     3061 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa
     3121 ttaattcaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     3181 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     3241 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     3301 tttggactag tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     3361 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     3421 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     3481 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     3541 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     3601 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     3661 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     3721 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     3781 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     3841 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg
     3901 ccgcagatct ctcgaggttg atctaatacg actcactata gggagaccca agctggctag
     3961 ttaagctatc aacaagtttg tacaaaaaag ttggcatggt gggccccgcg ccgcggcggc
     4021 ggctgcggcc gctggcagcg ctggccctgg tcctggcgct ggccccgggg ctgcccacag
     4081 cccgggccgg gcagacaccg cgccctgccg agcgggggcc cccagtgcgg cttttcaccg
     4141 aggaggagct ggcccgctat ggcggggagg aggaagatca gcccatctac ttggcagtga
     4201 agggagtggt gtttgatgtc acctccggaa aggagttcta cggcagagga gccccctaca
     4261 atgccttgac ggggaaggac tccactagag gggtagccaa gatgtccttg gatcctgcag
     4321 acctcaccca tgacactacg ggtctcacgg ccaaggaact ggaggccctg gatgaggtct
     4381 tcaccaaagt gtacaaagcc aaatacccca tcgtcggcta cactgcccgg agaattctca
     4441 atgaggatgg cagccctaac ctggacttca agcctgaaga ccagccccat tttgacatca
     4501 aggatgagtt ctgcccaact ttcttgtaca aagtggttga tctagagggc ccgcggttcg
     4561 aactcgagga ctacaaggac gacgatgaca agtcggccgc tggaggatac ccctacgacg
     4621 tgcccgacta cgcctagaat tccgcccccc ccccctaacg ttactggccg aagccgcttg
     4681 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     4741 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     4801 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     4861 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     4921 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     4981 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     5041 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     5101 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     5161 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac
     5221 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     5281 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     5341 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     5401 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     5461 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     5521 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     5581 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     5641 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     5701 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     5761 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     5821 atgcatcgat ggatcctaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     5881 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     5941 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     6001 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     6061 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     6121 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     6181 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     6241 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     6301 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     6361 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     6421 ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     6481 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     6541 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6601 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6661 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6721 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     6781 gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta gatcattctt
     6841 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     6901 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6961 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     7021 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     7081 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     7141 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     7201 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     7261 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
     7321 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     7381 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     7441 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     7501 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7561 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7621 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7681 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7741 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7801 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7861 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7921 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     7981 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     8041 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     8101 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     8161 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     8221 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     8281 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     8341 ccgggttgga ctcaagac
//
