LOCUS       sequence-256209-        9594 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral constitutive expression of CRISPR-resistant PINK1 with 
            c-terminal FLAG and HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9594)
  AUTHORS   Moutaoufik MT, Malty R, Amin S, Zhang Q, Phanse S, Gagarinova A, 
            Zilocchi M, Hoell L, Minic Z, Gagarinova M, Aoki H, Stockwell J, 
            Jessulat M, Goebels F, Broderick K, Scott NE, Vlasblom J, Musso G, 
            Prasad B, Lamantea E, Garavaglia B, Rajput A, Murayama K, Okazaki Y,
            Foster LJ, Bader GD, Cayabyab FS, Babu M
  TITLE     Rewiring of the Human Mitochondrial Interactome during Neuronal 
            Reprogramming Reveals Regulators of the Respirasome and 
            Neurogenesis.
  JOURNAL   iScience. 2019 Sep 4;19:1114-1132. doi: 10.1016/j.isci.2019.08.057.
  PUBMED    31536960
REFERENCE   2  (bases 1 to 9594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2019-09-4"; volume: "19:1114-1132. doi"; pages: " 
            10.1016/j.isci.2019.08.057"
COMMENT     Sequence Label: pPHAGE-CR-PINK1-C-TAP
FEATURES             Location/Qualifiers
     source          1..9594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     206..225
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     459..476
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    716..737
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(752..757,758..775,776..782)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    790..806
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     795..817
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     814..830
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     814..830
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     880..899
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1091..1724
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1771..1896
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2393..2626
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2811..2855
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3152..3269
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3321..3624
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3625..3828
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3779..3799
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3825..3849
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     3924..3943
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3924..3942
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3972..3996
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4002..5744
                     /codon_start=1
                     /product="human PTEN-induced putative kinase 1, a 
                     mitochondria serine/threonine protein kinase"
                     /label=PINK1
                     /translation="MAVRQALGRGLQLGRALLLRFTGKPGRAYGLGRPGPAAGCVRGER
                     PGWAAGPGAEPRRVGLGLPNRLRFFRQSVAGLAARLQRQFVVRAWGCAGPCGRAVFLAF
                     GLGLGLIEEKQAESRRAVSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIG
                     KGCSAAVYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERAAGAPAFPLAIKMMW
                     NISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLAPHPNIIRVLRAF
                     TSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAA
                     MMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLP
                     FSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGK
                     AHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHIL
                     ALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCS
                     WRAAL"
     protein_bind    complement(5749..5773)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5805..5828
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5844..5870
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             5844..5870
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    5877..6444
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5894..6446
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6038..6055)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6265..6284
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6458..7057
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6458..6477)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6954..6974
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7074..7662
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7127..7147)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7545..7556)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7737..7970
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8089..8107)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8175..8279
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8280..8348,8349..9140)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8498..8517)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9311..9594,1..305)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
       61 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      121 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      181 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      241 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      301 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      361 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      421 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      481 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      541 gcaagctcat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
      601 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
      661 ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
      721 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
      781 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga
      841 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
      901 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
      961 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
     1021 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     1081 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
     1141 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
     1201 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
     1261 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
     1321 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
     1381 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
     1441 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
     1501 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
     1561 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     1621 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     1681 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     1741 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     1801 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     1861 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     1921 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     1981 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     2041 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     2101 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     2161 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     2221 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     2281 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     2341 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     2401 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     2461 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     2521 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     2581 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     2641 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     2701 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     2761 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     2821 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     2881 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     2941 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     3001 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     3061 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa
     3121 ttaattcaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     3181 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     3241 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     3301 tttggactag tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     3361 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     3421 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     3481 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     3541 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     3601 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     3661 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     3721 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     3781 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     3841 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg
     3901 ccgcagatct ctcgaggttg atctaatacg actcactata gggagaccca agctggctag
     3961 ttaagctatc aacaagtttg tacaaaaaag caggctccac catggcggtg cgacaagcgc
     4021 tgggccgcgg cctgcagctg ggtcgagcgc tgctgctgcg cttcacgggc aagcccggcc
     4081 gggcctacgg cttggggcgg ccgggcccgg cggcgggctg tgtccgcggg gagcgtccag
     4141 gctgggccgc aggaccgggc gcggagcctc gcagggtcgg gctcgggctc cctaaccgtc
     4201 tccgcttctt ccgccagtcg gtggccgggc tggcggcgcg gttgcagcgg cagttcgtgg
     4261 tgcgggcctg gggctgcgcg ggcccttgcg gccgggcagt ctttctggcc ttcgggctag
     4321 ggctgggcct catcgaggaa aaacaggcgg agagccggcg ggcggtctcg gcctgtcagg
     4381 agatccaggc aatttttacc cagaaaagca agccggggcc tgacccgttg gacacgagac
     4441 gcttgcaggg ctttcggctg gaggagtatc tgatagggca gtccattggt aagggctgca
     4501 gtgctgctgt gtatgaagcc accatgccta cattgcccca gaacctggag gtgacaaaga
     4561 gcaccgggtt gcttccaggg agaggcccag gtaccagtgc accaggagaa gggcaggagc
     4621 gagctgcggg ggcccctgcc ttccccttgg ccatcaagat gatgtggaac atctcggcag
     4681 gttcctccag cgaagccatc ttgaacacaa tgagccagga gctggtccca gcgagccgag
     4741 tggccttggc tggggagtat ggagcagtca cttacagaaa atccaagaga ggtcccaagc
     4801 aactagcccc tcaccccaac atcatccggg ttctccgcgc cttcacctct tccgtgccgc
     4861 tgctgccagg ggccctggtc gactaccctg atgtgctgcc ctcacgcctc caccctgaag
     4921 gcctgggcca tggccggacg ctgttcctcg ttatgaagaa ctatccctgt accctgcgcc
     4981 agtacctttg tgtgaacaca cccagccccc gcctcgccgc catgatgctg ctgcagctgc
     5041 tggaaggcgt ggaccatctg gttcaacagg gcatcgcgca cagagacctg aaatccgaca
     5101 acatccttgt ggagctggac ccagacggct gcccctggct ggtgatcgca gattttggct
     5161 gctgcctggc tgatgagagc atcggcctgc agttgccctt cagcagctgg tacgtggatc
     5221 ggggcggaaa cggctgtctg atggccccag aggtgtccac ggcccgtcct ggccccaggg
     5281 cagtgattga ctacagcaag gctgatgcct gggcagtggg agccatcgcc tatgaaatct
     5341 tcgggcttgt caatcccttc tacggccagg gcaaggccca ccttgaaagc cgcagctacc
     5401 aagaggctca gctacctgca ctgcccgagt cagtgcctcc agacgtgaga cagttggtga
     5461 gggcactgct ccagcgagag gccagcaaga gaccatctgc ccgagtagcc gcaaatgtgc
     5521 ttcatctaag cctctggggt gaacatattc tagccctgaa gaatctgaag ttagacaaga
     5581 tggttggctg gctcctccaa caatcggccg ccactttgtt ggccaacagg ctcacagaga
     5641 agtgttgtgt ggaaacaaaa atgaagatgc tctttctggc taacctggag tgtgaaacgc
     5701 tctgccaggc agccctcctc ctctgctcat ggagggcagc cctgttggac ccagctttct
     5761 tgtacaaagt ggttgatcta gagggcccgc ggttcgaact cgaggactac aaggacgacg
     5821 atgacaagtc ggccgctgga ggatacccct acgacgtgcc cgactacgcc tagaattccg
     5881 cccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     5941 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     6001 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     6061 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     6121 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     6181 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     6241 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     6301 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     6361 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     6421 ttcctttgaa aaacacgatg ataatatggc cacaaccatg accgagtaca agcccacggt
     6481 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     6541 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     6601 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     6661 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     6721 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     6781 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     6841 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     6901 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     6961 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     7021 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgatgc atcgatggat cctaatcaac
     7081 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     7141 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     7201 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     7261 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     7321 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     7381 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     7441 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     7501 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     7561 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     7621 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc ctttaagacc
     7681 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7741 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     7801 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7861 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7921 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     7981 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     8041 gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac
     8101 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     8161 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     8221 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     8281 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     8341 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     8401 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     8461 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     8521 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     8581 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     8641 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     8701 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     8761 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     8821 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     8881 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     8941 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     9001 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     9061 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     9121 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     9181 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     9241 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9301 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9361 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     9421 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     9481 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     9541 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agac
//
