LOCUS       sequence-256219-        3421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SapTrap mNG-C1 donor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3421)
  TITLE     Unpublished SapTrap/SEC plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDD346
FEATURES             Location/Qualifiers
     source          1..3421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      983..992
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1546..1645)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1662..1681)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1663..1681)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1686..1702)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1686..1702)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1804..1823)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1815..2624
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1882..1901)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2771..3359
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3260..3279
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctgc tcttcgatgg tctccaaggg agaggaggac aacatggcct ccctcccagc
      721 cacccacgag ctccacatct tcggatccat caacggagtc gacttcgaca tggtcggaca
      781 aggaaccgga aacccaaacg acggatacga ggagctcaac ctcaagtcca ccaaggtaag
      841 tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagggag acctccaatt
      901 ctccccatgg atcctcgtcc cacacatcgg atacggattc caccaatacc tcccataccc
      961 agacggaatg tccccattcc aagccgccat ggtcgacgga tccggatacc aagtccaccg
     1021 taccatgcaa ttcgaggacg gagcctccct caccgtcaac taccgttaca cctacgaggg
     1081 atcccacatc aaggtaagtt taaacagttc ggtactaact aaccatacat atttaaattt
     1141 tcagggagag gcccaagtca agggaaccgg attcccagcc gacggaccag tcatgaccaa
     1201 ctccctcacc gccgccgact ggtgccgttc caagaagacc tacccaaacg acaaggtaag
     1261 tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagacca tcatctccac
     1321 cttcaagtgg tcctacacca ccggaaacgg aaagcgttac cgttccaccg cccgtaccac
     1381 ctacaccttc gccaagccaa tggccgccaa ctacctcaag aaccaaccaa tgtacgtctt
     1441 ccgtaagacc gagctcaagc actccaagac cgagctcaac ttcaaggagt ggcaaaaggc
     1501 cttcaccgac gtcatgggaa tggacgagct ctacaagcga agagcaccca gctttcttgt
     1561 acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta
     1621 tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
     1681 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     1741 ttgcacaaga taaaataata tcatcatgaa caataaaact gtctgcttac ataaacagta
     1801 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
     1861 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     1921 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     1981 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     2041 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     2101 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     2161 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     2221 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata
     2281 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     2341 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     2401 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     2461 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     2521 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     2581 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
     2641 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa
     2701 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     2761 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     2821 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2881 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     2941 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3001 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3061 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3121 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3181 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3241 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3301 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3361 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     3421 t
//
