LOCUS       sequence-256274-       10934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To make virus expressing Doc2beta D303N mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10934)
  AUTHORS   Courtney NA, Briguglio JS, Bradberry MM, Greer C, Chapman ER
  TITLE     Excitatory and Inhibitory Neurons Utilize Different Ca(2+) Sensors 
            and Sources to Regulate Spontaneous Release.
  JOURNAL   Neuron. 2018 Jun 6;98(5):977-991.e5. doi: 
            10.1016/j.neuron.2018.04.022. Epub 2018 May 10.
  PUBMED    29754754
REFERENCE   2  (bases 1 to 10934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2018.04.022"; journalName: "Neuron"; date: 
            "2018-06-6- 6"; volume: "98"; issue: "5"; pages: "977-991.e5"
COMMENT     Sequence Label: pFSW Doc2beta D303N mutant
FEATURES             Location/Qualifiers
     source          1..10934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..200
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             218..398
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    445..570
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1063..1296
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1481..1525
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1823..1940
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1985..2431
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    3693..4264
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3714..4266
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3858..3875)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4085..4104
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4266..4268,4269..4271,4272..4985)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4266..4268,4269..4271,4272..4982)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4311..4332)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4572..4591)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4919..4940
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5009..5597
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5062..5082)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5480..5491)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5600..5616)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5601..5617)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6122..6302
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6328..6345)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6334..6558
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6604..7032
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6691..6710)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6901..6922
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7041..7061)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7046..7375
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7226..7361
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7288..7307
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7423..7470
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7489..7863
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7993..8114
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8030..8049)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8084..8103
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8163..8179)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8163..8179)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8176..8198)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8187..8203
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8211..8217,8218..8235,8236..8241))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8256..8277
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8394..8411)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8565..9153)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8645..8664)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9324..10115,10116..10184))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9947..9966
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10185..10289)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10364..10383)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10555..10934
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat
      241 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      301 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      361 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      421 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      481 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      541 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      601 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
      661 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
      721 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
      781 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
      841 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
      901 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
      961 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1021 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1081 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1141 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1201 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1261 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1321 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1381 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1441 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1501 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1561 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1621 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1681 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1741 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     1801 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     1861 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     1921 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttaa
     1981 ttaaagtgca agtggtttta ggaccaggat gaggcggggt gggggtgcct acctgacgac
     2041 cgaccccgac ccactggaca agcacccaac ccccattccc caaattgcgc atcccctatc
     2101 agagaggggg aggggaaaca ggatgcggcg aggcgcgtgc gcactgccag cttcagcacc
     2161 gcggacagtg ccttcgcccc cgcctggcgg cgcgcgccac cgccgcctca gcactgaagg
     2221 cgcgctgacg tcactcgccg gtcccccgta aactcccctt cccggccacc ttggtcgcgt
     2281 ccgcgccgcc gccggcccag ccggaccgca ccacgcgagg cgcgagatag gggggcacgg
     2341 gcgcgaccat ctgcgctgcg gcgccggcga ctcagcgctg cctcagtctg cggtgggcag
     2401 cggaggagtc gtgtcgtgcc tgagagcgca ggtacggcta ggccaccatg accctccgaa
     2461 gacgtggaga gaaggctaca atcagcatcc aagagcatat ggcaatcgac gtgtgtcctg
     2521 gacctatccg acctatcaag cagatctccg attacttccc acgattccct cgtggactcc
     2581 caccaacagc tgcaccacgt gcttcagcac ctccagacgc tcctgcacga tcgccagcag
     2641 ctaccgcagg tcctcgtagc ccctccgacg gagcacgcga cgacgacgaa gatgtggacc
     2701 agctcttcgg agcctacggt gccagcccag gccccagccc cggacccagc cccgtgaggc
     2761 cgccggccaa gccccccgag gacgaaccgg acgccgacgg ctacgagtca gacgactgca
     2821 ccgccctggg tacactggac ttcagtctgc tctatgacca ggagaacaac gcactgcact
     2881 gcaccatcag caaagccaag ggcctgaagc cgatggatca caatggactg gctgatccct
     2941 acgtcaaact acacttgctg cctggagcca gcaaggcaaa taagctcaga acaaaaaccc
     3001 tacggaacac tctgaacccc tcctggaacg agaccctcac ctattacggg atcacggatg
     3061 aggacatgat ccgaaagacc ctgaggatct ccgtgtgtga cgaggacaaa ttccgccaca
     3121 acgagttcat cggagagact cgagtgcccc tgaagaagct gaaacccaac cacaccaaga
     3181 cattcagcat ctgcctggag aagcagctgc cggtggacaa ggcagaggac aagtccctgg
     3241 aggagcgtgg ccgcatcctc atctctctca agtacagctc acagaagcag ggcctgctgg
     3301 tgggcatcgt tcgctgcgca cacctggcgg ccatggatgc taacggctac tcaaacccct
     3361 acgtgaaaac atatctgaaa ccagatgtag acaagaaatc caagcataag accgcagtca
     3421 agaagaaaac actaaaccct gaattcaatg aggagttctg ttacgagatc aagcatggag
     3481 acctagccaa aaagaccctg gaggtcactg tctgggacta tgatattgga aaatccaatg
     3541 atttcatcgg tggggtggtt ctgggcatca atgccaaggg tgagcgcctg aagcactggt
     3601 ttgactgtct gaagaacaag gacaagagga ttgagcgttg gcacacgctc accaatgaga
     3661 tcccaggggc tgtactcagc gactaggaat tccccctctc cctccccccc ctaacgttac
     3721 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     3781 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     3841 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     3901 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     3961 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     4021 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     4081 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     4141 ttgtatggga tctgatctgg ggcctcggta cacatgcttt acatgtgttt agtcgaggtt
     4201 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     4261 ataatatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     4321 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     4381 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     4441 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     4501 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     4561 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     4621 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     4681 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     4741 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     4801 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     4861 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     4921 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     4981 agtaaaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     5041 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     5101 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     5161 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     5221 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     5281 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     5341 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     5401 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     5461 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     5521 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     5581 ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat
     5641 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     5701 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     5761 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     5821 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     5881 ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta
     5941 caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg
     6001 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg
     6061 gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc cggactgtac
     6121 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6181 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6241 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6301 cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     6361 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     6421 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     6481 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     6541 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     6601 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     6661 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6721 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     6781 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     6841 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     6901 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     6961 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     7021 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     7081 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     7141 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7201 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     7261 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     7321 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     7381 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     7441 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
     7501 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     7561 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     7621 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     7681 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
     7741 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
     7801 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
     7861 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     7921 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     7981 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8041 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8101 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     8161 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8221 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     8281 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     8341 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     8401 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     8461 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8521 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8581 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8641 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8701 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8761 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     8821 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     8881 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     8941 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9001 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9061 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9121 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9181 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9241 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     9301 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9361 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9421 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     9481 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     9541 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9601 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     9661 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     9721 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     9781 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     9841 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     9901 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     9961 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10021 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10081 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10141 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10201 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    10261 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    10321 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
    10381 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
    10441 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
    10501 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
    10561 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
    10621 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
    10681 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
    10741 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
    10801 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
    10861 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
    10921 tcgctattac catg
//
