LOCUS       sequence-256285-       11702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  URA3ex_CrisprCas9-yl_RFP: CAS9 vector with URA3ex marker for gRNA 
            cloning using GoldenGate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11702)
  AUTHORS   Larroude M, Trabelsi H, Nicaud JM, Rossignol T
  TITLE     A set of Yarrowia lipolytica CRISPR/Cas9 vectors for exploiting 
            wild-type strain diversity.
  JOURNAL   Biotechnol Lett. 2020 Jan 23. pii: 10.1007/s10529-020-02805-4. doi: 
            10.1007/s10529-020-02805-4.
  PUBMED    31974649
REFERENCE   2  (bases 1 to 11702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Lett. 2020 Jan 23. pii: 10.1007/s10529-020-02805-4. doi: 
            10.1007/s10529-020-02805-4."
COMMENT     Sequence Label: JME4472
FEATURES             Location/Qualifiers
     source          1..11702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      12..69
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(93..110)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(264..852)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(344..363)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1023..1814,1815..1883))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1646..1665
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1884..1988)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(2078..2121)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     protein_bind    2700..2721
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2736..2741,2742..2759,2760..2766)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2774..2790
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2808..2819
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2826..3503
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      3548..3619
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3635..3662
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    complement(3988..4021)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5321..5343)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5443..5462
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5656..5678
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5670..5687
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5671..5687
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             7294..11397
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7294..11397
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             11410..11430
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      11441..11688
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(11457..11475)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 tcttcctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
       61 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
      121 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      181 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      241 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
      301 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      361 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      421 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      481 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      541 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      601 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      661 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
      721 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
      781 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
      841 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
      901 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
      961 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1021 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1081 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1141 cccagtgctg caatgatacc gcgcgaccca cgctcaccgg ctccagattt atcagcaata
     1201 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1261 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1321 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     1381 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     1441 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     1501 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     1561 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     1621 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     1681 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     1741 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     1801 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     1861 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     1921 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     1981 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     2041 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
     2101 gctttcgcta aggatgattt ctggaattcg aagagcctct gacctgacgt ccccagttgc
     2161 aaaagttgac acaactctag atctgcttcc aaatatagaa tcataacaag ggttagggtg
     2221 tgattatata atattggtct taattgatgt gctagggctt taaaagttgg ttaaaataac
     2281 gctctaatgc ctttttaata tattgtcttt ttcaaaatct caaatcggac acttcttcgt
     2341 gtatgagact ccattttttg gctccgtcac gtgatatgta ttatcagcta tagtggtgta
     2401 aacaaagttt tttactagct gtaatggcat tttgtcggag tggtaaatcg ccttcttgtt
     2461 gtgcgttcga gttctggact ctgcactggg ctactttgaa aaatacctct aatgcgccga
     2521 tggtttagtg gtaaaatcca tcgttgccat cgatgggccc ccggttcgag agacggggcc
     2581 ggccatctgg gccctagagc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2641 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     2701 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     2761 atgttgtgtg gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat
     2821 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg
     2881 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg
     2941 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg
     3001 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca
     3061 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact
     3121 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca
     3181 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa
     3241 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag
     3301 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa
     3361 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac
     3421 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag
     3481 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc
     3541 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     3601 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     3661 cgtttatata ggccatctgg gccgggggcg tctctgagct agaaatagca agttaaaata
     3721 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tttacgtcta
     3781 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggcccagatc
     3841 ctctagagtc gaagcggccg ctatgtctga taaaaggatg taacataggc aagctgctcg
     3901 tgagtgttga gtacgaacct tagatccaaa tcacccgcac ccacggatat acttgcttga
     3961 atatacagta gtatgcggcc gctaggtata acttcgtata gcatacatta tacgaagtta
     4021 ttctgaattc cgagaaacac aacaacatgc cccattggac agaccatgcg gatacacagg
     4081 ttgtgcagta ccatacatac tcgatcagac aggtcgtctg accatcatac aagctgaaca
     4141 gcgctccata cttgcacgct ctctatatac acagttaaat tacatatcca tagtctaacc
     4201 tctaacagtt aatcttctgg taagcctccc agccagcctt ctggtatcgc ttggcctcct
     4261 caataggatc tcggttctgg ccgtacagac ctcggccgac aattatgata tccgttccgg
     4321 tagacatgac atcctcaaca gttcggtact gctgtccgag agcgtcgccc ttgtcgtcaa
     4381 gacccacccc gggggtcaga ataagccagt cctcagagtc gcccttaggt cggttctggg
     4441 caatgaagcc aaccacaaac tcggggtcgg atcgggcaag ctcaatggtc tgcttggagt
     4501 actcgccagt ggccagagag cccttgcaag acagctcggc cagcatgagc agacctctgg
     4561 ccagcttctc gttgggagag gggactagga actccttgta ctgggagttc tcgtagtcag
     4621 acacgtcctc cttcttctgt tcagagacag tttcctcggc accagctcgc aggccagcaa
     4681 tgattccggt tccgggtaca ccgtgggcgt tggtgatatc ggaccactcg gcgattcggt
     4741 gacaccggta ctggtgcttg acagtgttgc caatatctgc gaactttctg tcctcgaaca
     4801 ggaagaaacc gtgcttaaga gcaagttcct tgagggggag cacagtgccg gcgtaggtga
     4861 agtcgtcaat gatgtcgata tgggtcttga tcatgcacac ataaggtccg accttatcgg
     4921 caagctcaat gagctccttg gtggtggtaa catccagaga agcacacagg ttggttttct
     4981 tggctgccac gagcttgagc actcgagcgg caaaggcgga cttgtggacg ttagctcgag
     5041 cttcgtagga gggcattttg gtggtgaaga ggagactgaa ataaatttag tctgcagaac
     5101 tttttatcgg aaccttatct ggggcagtga agtatatgtt atggtaatag ttacgagtta
     5161 gttgaactta tagatagact ggactatacg gctatcggtc caaattagaa agaacgtcaa
     5221 tggctctctg ggcgcgaatt cgtataactt cgtatagcag gagttatccg aagcgaacgg
     5281 ggccctttcg tcacgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     5341 cggtgacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     5401 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     5461 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     5521 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     5581 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     5641 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttcgagctcg
     5701 gtacccgctt ttcgtagata atggaataca aatggatatc cagagtatac acatggatag
     5761 tatacactga cacgacaatt ctgtatctct ttatgttaac tactgtgagg cgttaaatag
     5821 agcttgatat ataaaatgtt acatttcaca gtctgaactt ttgcagatta cctaatttgg
     5881 taagatatta attatgaact gaaagttgat ggcatcccta aatttgatga aagggggaac
     5941 ccccgggttc gaaggtacca aggaagcatg cggtacccga attcgcttcg atcgcgtatc
     6001 gatgatacgc gtccatggtg aggtgtctca caagtgccgt gcagtcccgc ccccacttgc
     6061 ttctctttgt gtgtagtgta cgtacattat cgagagggtt gttcccgccc acctcgatcc
     6121 ggcatgctga ggtgtctcac aagtgccgtg cagtcccgcc cccacttgct tctctttgtg
     6181 tgtagtgtac gtacattatc gagagggttg ttcccgccca cctcgatccg gcatgctgag
     6241 gtgtctcaca agtgccgtgc agtcccgccc ccacttgctt ctctttgtgt gtagtgtacg
     6301 tacattatcg agagggttgt tcccgcccac ctcgatccgg catgctgagg tgtctcacaa
     6361 gtgccgtgca gtcccgcccc cacttgcttc tctttgtgtg tagtgtacgt acattatcga
     6421 gagggttgtt cccgcccacc tcgatccggc atgctgaggt gtctcacaag tgccgtgcag
     6481 tcccgccccc acttgcttct ctttgtgtgt agtgtacgta cattatcgag agggttgttc
     6541 ccgcccacct cgatccggca tgctgaggtg tctcacaagt gccgtgcagt cccgccccca
     6601 cttgcttctc tttgtgtgta gtgtacgtac attatcgaga gggttgttcc cgcccacctc
     6661 gatccggcat gctgaggtgt ctcacaagtg ccgtgcagtc ccgcccccac ttgcttctct
     6721 ttgtgtgtag tgtacgtaca ttatcgagag ggttgttccc gcccacctcg atccggcatg
     6781 ctgaggtgtc tcacaagtgc cgtgcagtcc cgcccccact tgcttctctt tgtgtgtagt
     6841 gtacgtacat tatcgagagg gttgttcccg cccacctcga tccggcatgc actgatcacg
     6901 ggcaaaagtg cttcgataga gagggggttg gcggcgcatt tgtgtcccaa aaaacagccc
     6961 caattgcccc aattgacccc aaattgaccc agtagcggac ccaaccccgg cgagagcccc
     7021 cttcacccca catatcaaac ctcccccggt tcccacactt gccgttaagg gcgtagggta
     7081 ctgcagtctg gaatctacgc ttgttcagac tttgtactag tttctttgtc tggccatccg
     7141 ggtaacccat gccggacgca aaatagacta ctgaaaattt ttttgctttg tggttgggac
     7201 tttagccaag ggtataaaag accaccgtcc ccgaattacc tttcctcttc ttttctctct
     7261 ctccttgtca actcacaccc gaagacaacc acaatggata agaaatactc cattggcctg
     7321 gacatcggaa ccaactccgt gggttgggcc gtgatcaccg atgagtacaa ggtgccctct
     7381 aagaaattca aggtcctggg caacaccgac cgacactcca tcaagaagaa cctgatcggc
     7441 gctctgctct tcgactctgg cgagactgct gaggccaccc gactgaagcg aaccgctcga
     7501 agacgataca cccgaagaaa gaaccgaatc tgttacctgc aggagatctt ctctaacgag
     7561 atggccaagg tggacgactc tttcttccac cgactggagg agtctttcct ggtggaggag
     7621 gacaagaagc acgagcgaca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac
     7681 gagaagtacc ccaccatcta ccacctgcga aagaagctgg tggactctac cgacaaggcc
     7741 gacctgcgac tgatctacct ggccctggcc cacatgatca agttccgagg ccacttcctg
     7801 atcgagggcg acctgaaccc cgacaactct gacgtggaca agctgttcat ccagctggtg
     7861 cagacctaca accagctctt cgaagagaac cccattaacg cttctggcgt ggatgctaag
     7921 gccatcctgt ctgcccgact gtctaagtct cgacgactcg agaacctgat tgctcagctc
     7981 cccggagaga agaagaacgg tctgttcgga aacctgattg ctctgtccct gggtctgacc
     8041 cctaacttca agtccaactt cgatctggct gaggacgcta agctgcagct gtctaaggac
     8101 acctacgacg atgacctgga taacctgctc gcccagattg gcgaccagta cgccgacctg
     8161 ttcctggccg ccaagaacct gtctgacgcc atcctgctgt ctgacatcct gcgagtgaac
     8221 accgagatca ccaaggcccc cctgtctgcc tccatgatta agcgatacga tgagcaccac
     8281 caggatctga ccctcctcaa ggctctggtc cgacagcagc tgcccgagaa gtacaaggag
     8341 attttcttcg accagtctaa gaacggctac gccggctaca tcgacggcgg cgcctctcag
     8401 gaggagttct acaagttcat taagcccatc ctggagaaga tggacggaac cgaggaactg
     8461 ctcgtgaagc tgaaccgaga ggacctcctg cgaaagcagc gaaccttcga caacggctct
     8521 atcccccacc agatccacct gggcgagctg cacgccatcc tgcgacgaca ggaggacttc
     8581 taccccttcc tgaaggacaa ccgagagaag atcgagaaga tcctgacctt ccgaatcccc
     8641 tactacgtgg gacccctggc ccgaggaaac tctcgattcg cttggatgac ccgaaagtct
     8701 gaggagacta ttaccccctg gaacttcgag gaggtggtgg ataagggcgc ctctgctcag
     8761 tctttcatcg agcgaatgac caacttcgac aagaacctcc ccaacgagaa ggtcctgccc
     8821 aagcactctc tgctctacga gtacttcacc gtctacaacg agctcaccaa ggtcaagtac
     8881 gtgaccgagg gaatgcgaaa gcccgctttc ctgtctggag agcagaagaa ggctattgtg
     8941 gatctgctct tcaagactaa ccgaaaggtc accgtcaagc agctgaagga ggattacttc
     9001 aagaagattg agtgtttcga ttctgtcgag atctccggcg tcgaggaccg attcaacgcc
     9061 tctctgggta cctaccacga cctgctgaag attatcaagg acaaggattt cctggataac
     9121 gaggagaacg aggatattct cgaggacatt gtcctgaccc tcaccctgtt cgaggatcga
     9181 gagatgattg aggagcgact caagacctac gctcacctgt tcgacgacaa ggtgatgaag
     9241 cagctgaagc gacgacgata caccggctgg ggccgactgt ctcgaaagct gatcaacggc
     9301 atccgagaca agcagtctgg caagaccatc ctggacttcc tgaagtctga cggcttcgcc
     9361 aaccgaaact tcatgcagct gatccacgac gactctctga ccttcaagga ggacatccag
     9421 aaggcccagg tgtctggcca gggcgactct ctgcacgagc acatcgccaa cctggccggc
     9481 tctcccgcca ttaagaaagg tatcctgcag accgtcaagg tggtcgatga gctcgtcaag
     9541 gtgatgggcc gacacaagcc cgagaacatt gtcattgaga tggctcgaga gaaccagact
     9601 actcagaagg gtcagaaaaa ctcccgagag cgaatgaagc gaattgagga aggtattaag
     9661 gagctgggct cccagattct caaggagcat cccgtggaga acactcagct ccagaacgag
     9721 aagctgtacc tgtactatct gcagaacggt cgagacatgt acgtcgacca ggagctggat
     9781 atcaaccgac tctccgacta cgatgtggac cacattgtgc cccagtcctt cctgaaggac
     9841 gattctatcg ataacaaggt gctgacccga tccgacaaga accgaggcaa gtctgacaac
     9901 gtgccctccg aggaggtggt caagaagatg aagaactact ggcgacagct gctgaacgcc
     9961 aagctgatta cccagcgaaa gttcgacaac ctgaccaagg ccgagcgagg cggcctgtct
    10021 gagctggaca aggccggctt catcaagcga cagctggtgg agactcgaca gatcaccaag
    10081 cacgtggccc agatcctgga ctctcgaatg aacaccaagt acgacgagaa cgacaagctg
    10141 atccgagagg tgaaggtgat caccctgaag tctaagctgg tgtctgactt ccgaaaggac
    10201 ttccagttct acaaggtgcg agagattaac aactaccacc acgcccacga tgcctacctg
    10261 aacgctgtcg tgggcaccgc cctcatcaag aagtatccca agctggagtc cgagttcgtc
    10321 tacggcgact acaaggtcta cgatgtgcga aaaatgattg ccaagtccga gcaggagatt
    10381 ggcaaggcta ccgccaagta cttcttctac tccaacatta tgaacttctt caagaccgag
    10441 attaccctgg ctaacggcga gattcgaaag cgacccctca ttgagactaa cggagagact
    10501 ggtgagatcg tgtgggacaa gggacgagac ttcgccaccg tgcgaaaggt gctgtctatg
    10561 ccccaggtga acatcgtgaa gaagaccgag gtgcagaccg gaggtttctc taaggagtcc
    10621 atcctgccca agcgaaactc tgacaagctg atcgcccgaa agaaggactg ggaccccaag
    10681 aagtacggag gtttcgactc tcccaccgtg gcttactctg tgctggtggt ggccaaggtg
    10741 gagaagggca agtctaagaa gctgaagtct gtgaaggagc tgctgggcat taccatcatg
    10801 gagcgatctt ctttcgagaa gaaccccatt gacttcctgg aggccaaggg atacaaggag
    10861 gtgaagaaag atctgattat caagctcccc aagtactctc tgttcgagct ggagaacgga
    10921 cgaaagcgaa tgctggcctc tgccggcgag ctgcagaagg gaaacgagct ggccctgccc
    10981 tccaagtacg tcaacttcct gtacctcgcc tcccattacg agaagctgaa gggctctccc
    11041 gaggataacg agcagaagca gctcttcgtg gagcagcata agcactacct ggacgagatc
    11101 atcgagcaga tttctgagtt ctctaagcga gtgatcctgg ctgacgccaa cctggataag
    11161 gtgctgtctg cttacaacaa gcaccgagac aagcccattc gagagcaggc tgagaacatc
    11221 attcacctgt tcaccctgac caacctggga gcccccgctg ccttcaagta cttcgacacc
    11281 accatcgacc gaaagcgata cacctctacc aaggaggtgc tggacgccac cctgatccac
    11341 cagtctatca ccggcctgta cgagactcga atcgacctgt ctcagctggg cggcgactct
    11401 cgagccgacc ccaagaagaa gcgaaaggtg taagctagcc tcatgtaatt agttatgtca
    11461 cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg agttagacaa
    11521 cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt
    11581 atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg
    11641 aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt taatttgcaa gcttggcggt
    11701 ct
//
