LOCUS       sequence-25632-e         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Ohta T, Michel JJ, Schottelius AJ, Xiong Y
  TITLE     ROC1, a homolog of APC11, represents a family of cullin partners 
            with an associated ubiquitin ligase activity.
  JOURNAL   Mol Cell. 1999 Apr . 3(4):535-41.
  PUBMED    10230407
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            1999 Apr . 3(4):535-41."
COMMENT     Sequence Label: pcDNA3-HA2-ROC1
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             83..109
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             113..139
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             119..145
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     regulatory      185..194
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      189..198
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     gap             565..580
                     /estimated_length=16
     gap             589..604
                     /estimated_length=16
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagcta tgtacccata cgatgttcct gactatgcgg gctatcccta
      121 tgacgtcccg gactatgcaa gcttggtacc gttgggctgc aggaattcgg cacgagcggc
      181 acgagccacc atggcggcag cgatggatgt ggataccccg agcggcacca acagcggcgc
      241 gggcaagaag cgctttgaag tgaaaaagtg gaatgcagta gccctctggg cctgggatat
      301 tgtggttgat aactgtgcca tctgcaggaa ccacattatg gatctttgca tagaatgtca
      361 agctaaccag gcgtccgcta cttcagaaga gtgtactgtc gcatggggag tctgtaacca
      421 tgcttttcac ttccactgca tctctcgctg gctcaaaaca cgacaggtgt gtccattgga
      481 caacagagag tgggaattcc aaaagtatgg gcactaggaa aagacttctt ccatcaagct
      541 taattgtttt gttattcatt taatnnnnnn nnnnnnnnnn annnnnntnn nnnnnnnnnn
      601 nnnngtgnnt tttttnnnnn ntntnttttt cnntngnngn nngnttctgn ancctnttgn
      661 attctgngtc aanaanncca gttg
//
