LOCUS       sequence-25633-e         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Ohta T, Michel JJ, Schottelius AJ, Xiong Y
  TITLE     ROC1, a homolog of APC11, represents a family of cullin partners 
            with an associated ubiquitin ligase activity.
  JOURNAL   Mol Cell. 1999 Apr . 3(4):535-41.
  PUBMED    10230407
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            1999 Apr . 3(4):535-41."
COMMENT     Sequence Label: pcDNA3-HA-APC11
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             83..109
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             113..139
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             119..145
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     promoter        complement(463..481)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(464..481)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(501..518)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     gap             559..572
                     /estimated_length=14
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagcta tgtacccata cgatgttcct gactatgcgg gctatcccta
      121 tgacgtcccg gactatgcaa gcttggtacc gttaacatgg atccccatga aggtgaagat
      181 taagtgctgg aacggcgtgg ccacttggct ctgggtggcc aacgatgaga actgtggcat
      241 ctgcaggatg gcatttaacg gatgctgccc tgactgcaag gtgcccggcg acgactgccc
      301 gctggtgtgg ggccagtgct cccactgctt ccacatgcat tgcatcctca agtggctgca
      361 cgcacagcag gtgcagcagc actgccccat gtgccgccag gaatggaagt tcaaggagtg
      421 aggcccgacc tggctctcga gcatgcatct agagggccct attctatagt gtcacctaaa
      481 tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
      541 cccctccccc gtgccttcnn nnnnnnnnnn nngnccnnnn nncnnnnnnn nntncctnnn
      601 nnnatgagga aattgcatcg cattgtctga gtangtgtca ttctattctg gggggtgggg
      661 tgg
//
