LOCUS       sequence-25634-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Ohta T, Michel JJ, Schottelius AJ, Xiong Y
  TITLE     ROC1, a homolog of APC11, represents a family of cullin partners 
            with an associated ubiquitin ligase activity.
  JOURNAL   Mol Cell. 1999 Apr . 3(4):535-41.
  PUBMED    10230407
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            1999 Apr . 3(4):535-41."
COMMENT     Sequence Label: pcDNA3-HA-ROC2
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             83..109
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             113..139
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             119..145
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     gap             577..595
                     /estimated_length=19
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagcta tgtacccata cgatgttcct gactatgcgg gctatcccta
      121 tgacgtcccg gactatgcaa gcttggtacc gttaacatgg atccccatgg ccgacgtgga
      181 agacggagag gaaacctgcg ccctggcctc tcactccggg agctcaggct ccaagtcggg
      241 aggcgacaag atgttctccc tcaagaagtg gaacgcggtg gccatgtgga gctgggacgt
      301 ggagtgcgat acgtgcgcca tctgcagggt ccaggtgatg gatgcctgtc ttagatgtca
      361 agctgaaaac aaacaagagg actgtgttgt ggtctgggga gaatgtaatc attccttcca
      421 caactgctgc atgtccctgt gggtgaaaca gaacaatcgc tgccctctct gccagcagga
      481 ctgggtggtc caaagaatcg gcaaatgaga gtggttagaa ggcttcttag cgcagttgtt
      541 cagagccctg gtggatcttg taatccagtg ccctacnnnn nnnnnnnnnn nnnnnannnn
      601 nntnnnnnna ntagganccn annnnnnnnc tcgagcatgn ntctananng nncnntnntn
      661 cnntanngnn ncctaaanng nnnnnnagct cgctgancan cctcgactgn gccttctant
      721 tgccagccat ctgtngnttg cccct
//
