LOCUS       sequence-256345-        9989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Piggybac vector for shRNA-mediated knockdown, co-expressed with 
            FUCCI cell cycle reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   Fischer AD, Veronese-Paniagua DA, Swaminathan S, Kashima H, Rubin 
            DC, Madison BB
  TITLE     The oncogenic function of PLAGL2 is mediated via ASCL2 and IGF2 and 
            a Wnt-independent mechanism in colorectal cancer.
  JOURNAL   Am J Physiol Gastrointest Liver Physiol. 2023 Jun 13. doi: 
            10.1152/ajpgi.00058.2022.
  PUBMED    37310750
REFERENCE   2  (bases 1 to 9989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J 
            Physiol Gastrointest Liver Physiol. 2023 Jun 13. doi: 
            10.1152/ajpgi.00058.2022."
COMMENT     Sequence Label: BII-Sh-FUCCI-Luc
FEATURES             Location/Qualifiers
     source          1..9989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..39
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(308..324)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(308..324)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        580..828
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     580..600
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     751..770
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     CDS             2203..2256
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2263..2289
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2263..2289
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(2296..2298,2299..2301,2302..3012)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(2341..2362)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     2949..2970
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3043..3372
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     misc_feature    3377..3949
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3544..3561)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3771..3790
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3964..4614
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAH"
     CDS             3964..4614
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAH"
     CDS             4669..4941
                     /codon_start=1
                     /product="degron consisting of residues 30-120 of human 
                     Cdt1 (Zielke and Edgar, 2015)"
                     /label=Cdt1 (30-120)
                     /translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
                     DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
     misc_feature    4966..5554
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5019..5039)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5437..5448)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5634..5768
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5658..5677)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5712..5731
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   6546..6608
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6738..6755)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6738..6754)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6747..6769)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6896..7351
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6983..7002)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7193..7214
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7377..7481
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7482..7550,7551..8342)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7700..7719)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8513..9101
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9002..9021
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9255..9272
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9389..9410
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9425..9430,9431..9448,9449..9455)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9463..9479
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9468..9490
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9487..9503
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9487..9503
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat ccactagttc tagagggaca gccccccccc aaagccccca gggatgtaat
      361 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
      421 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
      481 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
      541 ggatacgggg aaaaggcctc cagggccgcc cccttcaccg agggcctatt tcccatgatt
      601 ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat taatttgact
      661 gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag
      721 tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag
      781 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg tcacagaatc
      841 gtcgtatgca gctcgagctg catacgacga ttctgtgatt tttgcctcca aggcctgcag
      901 gagtcaatgg gaaaaaccca ttggagccaa gtacactgac tcaataggga ctttccattg
      961 ggttttgccc agtacataag gtcaataggg ggtgagtcaa caggaaagtc ccattggagc
     1021 caagtacatt gagtcaatag ggactttcca atgggttttg cccagtacat aaggtcaatg
     1081 ggaggtaagc caatgggttt ttcccattac tgacatgtat actgagtcat tagggacttt
     1141 ccaatgggtt ttgcccagta cataaggtca ataggggtga atcaacagga aagtcccatt
     1201 ggagccaagt acactgagtc aatagggact ttccattggg ttttgcccag tacaaaaggt
     1261 caataggggg tgagtcaatg ggtttttccc attattggca catacataag gtcaataggg
     1321 gtgactagtg gagaagagca tgcttgaggg ctgagtgccc ctcagtgggc agagagcaca
     1381 tggcccacag tccctgagaa gttgggggga ggggtgggca attgaactgg tgcctagaga
     1441 aggtggggct tgggtaaact gggaaagtga tgtggtgtac tggctccacc tttttcccca
     1501 gggtggggga gaaccatata taagtgcagt agtctctgtg aacattcggt ttagtgaacc
     1561 tgcaggtcgg ccgccacgac cggtgccacc atgactgaat acaaaccaac tgttcgcctg
     1621 gcaactcgtg atgatgttcc acgtgcagtt cgcaccctgg ctgctgcatt tgctgactac
     1681 cctgcaaccc gtcacactgt ggacccagac cgccacattg aacgtgtgac tgaactgcag
     1741 gagctgttcc tgacccgtgt gggcctggac attggcaaag tgtgggtggc agatgatggt
     1801 gctgctgtgg cagtgtggac cacccctgaa tctgttgaag ctggtgcagt gtttgctgag
     1861 attggcccac gcatggcaga actgtctggc agccgcctgg cagcacaaca gcagatggaa
     1921 ggtctgctgg caccacaccg cccaaaagaa cctgcttggt tcctggcaac tgtgggtgtg
     1981 agccctgacc accagggtaa gggcctgggc tctgcagtgg tgctgcctgg tgtggaagca
     2041 gctgaacgtg caggtgtgcc tgctttcctg gagacctcag ctccacgcaa cctgcctttc
     2101 tatgaacgcc tgggcttcac tgtgactgct gatgtggaag tgccagaagg cccacgcact
     2161 tggtgcatga ctcgcaaacc aggtgctagc cttggctccg gcgagggcag gggaagtctt
     2221 ctaacatgcg gggacgtgga ggaaaatccc ggcccactcg gctaccccta cgacgtgccc
     2281 gactacgcca gcggcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     2341 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtccgcgg cgagggcgag
     2401 ggcgatgcca ccaacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     2461 gtgccctggc ccaccctcgt gaccaccttc ggctacggcg tggcctgctt cagccgctac
     2521 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     2581 gagcgcacca tctctttcaa ggacgacggt acctacaaga cccgcgccga ggtgaagttc
     2641 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     2701 aacatcctgg ggcacaagct ggagtacaac ttcaacagcc acaacgtcta tatcacggcc
     2761 gacaagcaga agaacggcat caaggctaac ttcaagatcc gccacaacgt tgaggacggc
     2821 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     2881 ctgcccgaca accactacct gagccatcag tccgccctga gcaaagaccc caacgagaag
     2941 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggattacaca tggcatggac
     3001 gagctgtaca aggatatcca tgcacactgg cggccgctcg agatgaatcc cagtatgaag
     3061 cagaaacaag aagaaatcaa agagaatata aagaatagtt ctgtcccaag aagaactctg
     3121 aagatgattc agccttctgc atctggatct cttgttggaa gagaaaatga gctgtccgca
     3181 ggcttgtcca aaaggaaaca tcggaatgac cacttaacat ctacaacttc cagccctggg
     3241 gttattgtcc cagaatctag tgaaaataaa aatcttggag gagtcaccca ggagtcattt
     3301 gatcttatga ttaaagaaaa tccatcctct cagtattgga aggaagtggc agaaaaacgg
     3361 agaaaggcgc tgtaagcccc tctccctccc ccccccctaa cgttactggc cgaagccgct
     3421 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     3481 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     3541 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     3601 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     3661 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     3721 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     3781 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     3841 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aacgtctagg
     3901 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat ggccacagaa
     3961 ttcatggtga gcgtgatcaa gcccgagatg aagatgaggt actacatgga cggctccgtc
     4021 aatgggcatg agttcacaat cgagggtgag ggcacaggca gaccttacga gggacatcag
     4081 gagatgacac tgcgcgtcac aatggccgag ggcgggccaa tgcctttcgc cttcgacctg
     4141 gtgtcccacg tgttctgtta cggccacaga gtttttacca agtacccaga agagatccca
     4201 gactatttca agcaggcctt tcctgagggc ctgtcctggg agaggtccct ggagttcgag
     4261 gacggcggct ccgcctccgt gagcgcccac atcagcctga ggggcaacac cttctaccac
     4321 aagtccaagt tcaccggcgt gaacttcccc gccgacggcc ccatcatgca gaaccagagc
     4381 gtggactggg agccctccac cgagaagatc accgccagcg acggcgtgct gaagggcgac
     4441 gtgaccatgt acctgaagct ggagggcggc ggcaaccaca agtgccagat gaagaccacc
     4501 tacaaggccg ccaaggagat cctggagatg cccggcgacc actacatcgg ccacaggctg
     4561 gtgaggaaga ccgagggcaa catcaccgag caggtggagg acgccgtggc ccactactca
     4621 atgttgcctt ccctgggcga tatccatgca cactggcggc cgctcgagcc cagccccgcc
     4681 aggcccgcac tccgcgcccc ggcctccgct accagtggca gccgcaagcg cgcccgcccg
     4741 cccgccgccc ccggacgcga ccaggccagg ccaccggccc gcaggagact gcggctgtcg
     4801 gtggacgagg tttccagccc cagtaccccc gaggccccag acatcccagc ctgcccttct
     4861 ccgggccaga agataaagaa atccaccccg gcagcaggtc agccgcccca cctgacatcc
     4921 gcgcaggacc aggacaccat ctgagctaga gttcgcgtct ggaacaatca acctctggat
     4981 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     5041 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5101 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     5161 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     5221 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     5281 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     5341 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     5401 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     5461 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     5521 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgg ctaagcactt
     5581 cgtggccgtc gatcgtttaa agggaggtag tgagtcgaga tatcgatact agtgatccag
     5641 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     5701 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     5761 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     5821 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag
     5881 agggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg
     5941 cagcagcgag ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg
     6001 gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac
     6061 gcttctcgct gctctttgag cctgcagaca cctgggggga tacggggaaa agccatacca
     6121 atggagctca cggggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc
     6181 ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca
     6241 tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct
     6301 ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa
     6361 aaagaattcg ataaaagttt tgttacttta tagaagaaat tttgagtttt tgtttttttt
     6421 aataaataaa taaacataaa taaattgttt gttgaattta ttattagtat gtaagtgtaa
     6481 atataataaa acttaatatc tattcaaatt aataaataaa cctcgatata cagaccgata
     6541 aaacacatgc gtcaatttta cacatgatta tctttaacgt acgtcacaat atgattatct
     6601 ttctagggtt aatctagctg cgtgttctgc agcgtgtcga gcatcttcat ctgctccatc
     6661 acgctgtaaa acacatttgc accgcgagtc tgcccgtcct ccacgggttc aaaaacgtga
     6721 atgaacgagg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     6781 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     6841 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     6901 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6961 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     7021 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     7081 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     7141 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     7201 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     7261 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     7321 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     7381 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     7441 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     7501 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     7561 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     7621 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     7681 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     7741 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     7801 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     7861 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     7921 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     7981 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     8041 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     8101 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     8161 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     8221 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     8281 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     8341 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     8401 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     8461 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     8521 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     8581 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     8641 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     8701 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     8761 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     8821 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     8881 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     8941 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     9001 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     9061 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     9121 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     9181 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     9241 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     9301 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     9361 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     9421 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     9481 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcccgccgg gtaactcacg
     9541 gggtatccat gtccatttct gcggcatcca gccaggatac ccgtcctcgc tgacgtaata
     9601 tcccagcgcc gcaccgctgt cattaatctg cacaccggca cggcagttcc ggctgtcgcc
     9661 ggtattgttc gggttgctga tgcgcttcgg gctgaccatc cggaactgtg tccggaaaag
     9721 ccgcgacgaa ctggtatccc aggtggcctg aacgaacagt tcaccgttaa aggcgtgcat
     9781 ggccacacct tcccgaatca tcatggtaaa cgtgcgtttt cgctcaacgt caatgcagca
     9841 gcagtcatcc tcggcaaact ctttccatgc cgcttcaacc tcgcgggaaa aggcacgggc
     9901 ttcttcctcc ccgatgccca gatagcgcca gcttgggcga tgactgagcc ggaaaaaaga
     9961 cccgacgata tgatcctgat gcagctaga
//
