LOCUS       sequence-256351-        9469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Piggybac vector for constitutive expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9469)
  AUTHORS   Fischer AD, Veronese-Paniagua DA, Swaminathan S, Kashima H, Rubin 
            DC, Madison BB
  TITLE     The oncogenic function of PLAGL2 is mediated via ASCL2 and IGF2 and 
            a Wnt-independent mechanism in colorectal cancer.
  JOURNAL   Am J Physiol Gastrointest Liver Physiol. 2023 Jun 13. doi: 
            10.1152/ajpgi.00058.2022.
  PUBMED    37310750
REFERENCE   2  (bases 1 to 9469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J 
            Physiol Gastrointest Liver Physiol. 2023 Jun 13. doi: 
            10.1152/ajpgi.00058.2022."
COMMENT     Sequence Label: BII-ChBtW-iresFUCCI
FEATURES             Location/Qualifiers
     source          1..9469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..39
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(308..324)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(308..324)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      1257..1266
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1263..1658
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             1677..1730
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1740..1763
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(1776..1778,1779..1781,1782..2492)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(1821..1842)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     2429..2450
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2523..2852
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     misc_feature    2857..3429
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3024..3041)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3251..3270
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3444..4094
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAH"
     CDS             3444..4094
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAH"
     CDS             4149..4421
                     /codon_start=1
                     /product="degron consisting of residues 30-120 of human 
                     Cdt1 (Zielke and Edgar, 2015)"
                     /label=Cdt1 (30-120)
                     /translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
                     DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
     misc_feature    4446..5034
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4499..4519)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4917..4928)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5114..5248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5138..5157)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5192..5211
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   6026..6088
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6218..6235)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6218..6234)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6227..6249)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6376..6831
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6463..6482)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6673..6694
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6857..6961
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6962..7030,7031..7822)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7180..7199)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7993..8581
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8482..8501
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8735..8752
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8869..8890
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8905..8910,8911..8928,8929..8935)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8943..8959
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8948..8970
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8967..8983
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8967..8983
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat ccactagttc tagagggaca gccccccccc aaagccccca gggatgtaat
      361 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
      421 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
      481 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
      541 ggatacgggg aaaaggcctc caaggcctgc aggagtcaat gggaaaaacc cattggagcc
      601 aagtacactg actcaatagg gactttccat tgggttttgc ccagtacata aggtcaatag
      661 ggggtgagtc aacaggaaag tcccattgga gccaagtaca ttgagtcaat agggactttc
      721 caatgggttt tgcccagtac ataaggtcaa tgggaggtaa gccaatgggt ttttcccatt
      781 actgacatgt atactgagtc attagggact ttccaatggg ttttgcccag tacataaggt
      841 caataggggt gaatcaacag gaaagtccca ttggagccaa gtacactgag tcaataggga
      901 ctttccattg ggttttgccc agtacaaaag gtcaataggg ggtgagtcaa tgggtttttc
      961 ccattattgg cacatacata aggtcaatag gggtgactag tggagaagag catgcttgag
     1021 ggctgagtgc ccctcagtgg gcagagagca catggcccac agtccctgag aagttggggg
     1081 gaggggtggg caattgaact ggtgcctaga gaaggtgggg cttgggtaaa ctgggaaagt
     1141 gatgtggtgt actggctcca cctttttccc cagggtgggg gagaaccata tataagtgca
     1201 gtagtctctg tgaacattcg gtttagtgaa cctgcaggtc ggccgccacg accggtgcca
     1261 ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa
     1321 tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg
     1381 gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg
     1441 tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg
     1501 gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc
     1561 tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga
     1621 ttcgtgaatt gctgccctct ggttatgtgt gggagggcgc tagccttggc tccggcgagg
     1681 gcaggggaag tcttctaaca tgcggggacg tggaggaaaa tcccggccca ctcggcatgg
     1741 actacaagga cgacgatgac aagctcgata gcggcatggt gagcaagggc gaggagctgt
     1801 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     1861 gcgtccgcgg cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg aagttcatct
     1921 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcg
     1981 tggcctgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     2041 tgcccgaagg ctacgtccag gagcgcacca tctctttcaa ggacgacggt acctacaaga
     2101 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     2161 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac ttcaacagcc
     2221 acaacgtcta tatcacggcc gacaagcaga agaacggcat caaggctaac ttcaagatcc
     2281 gccacaacgt tgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     2341 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagccatcag tccgccctga
     2401 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     2461 ggattacaca tggcatggac gagctgtaca aggatatcca tgcacactgg cggccgctcg
     2521 agatgaatcc cagtatgaag cagaaacaag aagaaatcaa agagaatata aagaatagtt
     2581 ctgtcccaag aagaactctg aagatgattc agccttctgc atctggatct cttgttggaa
     2641 gagaaaatga gctgtccgca ggcttgtcca aaaggaaaca tcggaatgac cacttaacat
     2701 ctacaacttc cagccctggg gttattgtcc cagaatctag tgaaaataaa aatcttggag
     2761 gagtcaccca ggagtcattt gatcttatga ttaaagaaaa tccatcctct cagtattgga
     2821 aggaagtggc agaaaaacgg agaaaggcgc tgtaagcccc tctccctccc ccccccctaa
     2881 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     2941 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     3001 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     3061 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     3121 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     3181 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     3241 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     3301 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     3361 gaggttaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     3421 cgatgataat ggccacagaa ttcatggtga gcgtgatcaa gcccgagatg aagatgaggt
     3481 actacatgga cggctccgtc aatgggcatg agttcacaat cgagggtgag ggcacaggca
     3541 gaccttacga gggacatcag gagatgacac tgcgcgtcac aatggccgag ggcgggccaa
     3601 tgcctttcgc cttcgacctg gtgtcccacg tgttctgtta cggccacaga gtttttacca
     3661 agtacccaga agagatccca gactatttca agcaggcctt tcctgagggc ctgtcctggg
     3721 agaggtccct ggagttcgag gacggcggct ccgcctccgt gagcgcccac atcagcctga
     3781 ggggcaacac cttctaccac aagtccaagt tcaccggcgt gaacttcccc gccgacggcc
     3841 ccatcatgca gaaccagagc gtggactggg agccctccac cgagaagatc accgccagcg
     3901 acggcgtgct gaagggcgac gtgaccatgt acctgaagct ggagggcggc ggcaaccaca
     3961 agtgccagat gaagaccacc tacaaggccg ccaaggagat cctggagatg cccggcgacc
     4021 actacatcgg ccacaggctg gtgaggaaga ccgagggcaa catcaccgag caggtggagg
     4081 acgccgtggc ccactactca atgttgcctt ccctgggcga tatccatgca cactggcggc
     4141 cgctcgagcc cagccccgcc aggcccgcac tccgcgcccc ggcctccgct accagtggca
     4201 gccgcaagcg cgcccgcccg cccgccgccc ccggacgcga ccaggccagg ccaccggccc
     4261 gcaggagact gcggctgtcg gtggacgagg tttccagccc cagtaccccc gaggccccag
     4321 acatcccagc ctgcccttct ccgggccaga agataaagaa atccaccccg gcagcaggtc
     4381 agccgcccca cctgacatcc gcgcaggacc aggacaccat ctgagctaga gttcgcgtct
     4441 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     4501 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4561 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     4621 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     4681 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     4741 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     4801 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     4861 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     4921 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     4981 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     5041 ttaattctgg ctaagcactt cgtggccgtc gatcgtttaa agggaggtag tgagtcgaga
     5101 tatcgatact agtgatccag acatgataag atacattgat gagtttggac aaaccacaac
     5161 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     5221 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     5281 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     5341 ggctgattat gatcctctag agggacagcc cccccccaaa gcccccaggg atgtaattac
     5401 gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg gtccggcgct
     5461 ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac
     5521 gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca cctgggggga
     5581 tacggggaaa agccatacca atggagctca cggggacagc ccccccccaa agcccccagg
     5641 gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg gctccgctcc
     5701 ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg
     5761 gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga gcctgcagac
     5821 acctgggggg atacggggaa aaagaattcg ataaaagttt tgttacttta tagaagaaat
     5881 tttgagtttt tgtttttttt aataaataaa taaacataaa taaattgttt gttgaattta
     5941 ttattagtat gtaagtgtaa atataataaa acttaatatc tattcaaatt aataaataaa
     6001 cctcgatata cagaccgata aaacacatgc gtcaatttta cacatgatta tctttaacgt
     6061 acgtcacaat atgattatct ttctagggtt aatctagctg cgtgttctgc agcgtgtcga
     6121 gcatcttcat ctgctccatc acgctgtaaa acacatttgc accgcgagtc tgcccgtcct
     6181 ccacgggttc aaaaacgtga atgaacgagg cgcgctcact ggccgtcgtt ttacaacgtc
     6241 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     6301 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     6361 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6421 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6481 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6541 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6601 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6661 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     6721 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     6781 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     6841 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6901 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6961 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     7021 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     7081 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     7141 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     7201 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7261 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     7321 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     7381 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7441 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7501 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7561 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7621 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7681 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7741 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7801 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7861 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7921 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7981 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8041 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8101 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     8161 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8221 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8281 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8341 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8401 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8461 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8521 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8581 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8641 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8701 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8761 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     8821 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     8881 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     8941 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     9001 cgcccgccgg gtaactcacg gggtatccat gtccatttct gcggcatcca gccaggatac
     9061 ccgtcctcgc tgacgtaata tcccagcgcc gcaccgctgt cattaatctg cacaccggca
     9121 cggcagttcc ggctgtcgcc ggtattgttc gggttgctga tgcgcttcgg gctgaccatc
     9181 cggaactgtg tccggaaaag ccgcgacgaa ctggtatccc aggtggcctg aacgaacagt
     9241 tcaccgttaa aggcgtgcat ggccacacct tcccgaatca tcatggtaaa cgtgcgtttt
     9301 cgctcaacgt caatgcagca gcagtcatcc tcggcaaact ctttccatgc cgcttcaacc
     9361 tcgcgggaaa aggcacgggc ttcttcctcc ccgatgccca gatagcgcca gcttgggcga
     9421 tgactgagcc ggaaaaaaga cccgacgata tgatcctgat gcagctaga
//
