LOCUS       sequence-256356-       21179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  vab-1 rescuing minigene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21179)
  AUTHORS   George SE, Simokat K, Hardin J, Chisholm AD
  TITLE     The VAB-1 Eph receptor tyrosine kinase functions in neural and 
            epithelial morphogenesis in C. elegans.
  JOURNAL   Cell. 1998 Mar 6;92(5):633-43.
  PUBMED    9506518
REFERENCE   2  (bases 1 to 21179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1998-03-6"; volume: "92(5)"; pages: "633-43"
COMMENT     Sequence Label: pCZ47
FEATURES             Location/Qualifiers
     source          1..21179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4865..4882
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             6204..6221
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(7164..7178)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             12719..12739
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     primer_bind     complement(14524..14541)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14524..14540)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14533..14555)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      14753..15208
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14840..14859)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15050..15071
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(15225..15244)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15344..15366
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(15404..15422)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        15490..15594
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(15595..15663,15664..16455)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(15813..15832)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      16626..17214
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     17115..17134
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     17368..17385
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    17502..17523
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(17538..17543,17544..17561,17562..17568)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    17576..17592
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     17581..17603
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     17600..17616
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     17600..17616
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gggggggggg gggggggrkg tttcgttaga atacttttca aaaaaatttc aaaattttga
       61 aaaaaaagcc aaattctgca tttttcgtcg tacatatcac attaaatcgt tgatattggt
      121 atattttagg ttgagaggca tccaaattca aaaatattgg aacctaccaa aaaaagtgga
      181 atttgccaag ttttgaaatt taccaataaa ttggaattta ccaaaaaatt ttggaatata
      241 ccaaaattgg caattaccaa aatgttggta tttaccaatt gttggaatcg accaattttt
      301 cgaatttacc aaaaattgga atttatcaaa aattgtaatt cacctaataa taggagatta
      361 ccaaaaaatt ggaatttacc aaaatattgg ttttcaggtt tcagcagaag tttgcacttc
      421 ttccaatatt tagaaattta atataagaaa attcattatg gaattcggtc tgttgtcggg
      481 ctacgttcga gcaaaaagtg ctcttttcag aattccttca aatcttccat atgcccatca
      541 ccatcttccg gatctcatta tttgcaaccc ctccgttcaa aaaaaaagag atttatgact
      601 tccgcggtca gttcctttga tttctattcc catccgctgg gttttttcca cccaaaaccg
      661 caacaaaact aactttatcc ccagtgtaca aggacatttt gattgaaatg caaattcgcc
      721 ctcacttgga ggtaatgtaa ctgtctgttg tatgtccccc ctctaacaat catgtcgcca
      781 atcaagatgg aggcgaagaa gaaggtcggg aagggaaaca agaaaccgat aaagtcgaag
      841 ttagtgaagc ctcctctctt ccaattctcc acaggtgttc ttctatgttt atatgttcac
      901 tttcgctttt ccacatcatt ttccttcttt ttgaagtcaa atcggcgggc gatgaagaaa
      961 aaaagaaaag ccgccgccgc cgccctttcc gctttccaga agaaggggga ccccagaaag
     1021 aagtgaccgc aagtcatacc aggtgtgaca tatgaacaac tgctaagcgt atgtggtgag
     1081 agagatgggt gacaaattct ctagtcgtaa attttaacta acaatattgt cagcaaagaa
     1141 tgaggtgaaa agcagccaac tgaccagggt acgggctgcc gattgtttcg gtttttcggg
     1201 tcccttgact caaggaatct ctggtatgac ccatcgtaaa ccctttcaaa tatattgtta
     1261 acaatacaat acggcaagcc agctgacccg atctgatggg ttgcatgttc ttactcatta
     1321 ttctattatc ctcatcgtcg tcttcccgaa aacggtaaac attgtaaacc attctgggga
     1381 gcatttcttt ttgaatatcg ccgcgaaagg tcatgcacct gacaccatca tccattagaa
     1441 tgcacgaaat tcaagaagac catacagtct cttccgcatg cgcgtgtatg ttgtttgcgg
     1501 cattaccaga gagaaccgcg taatacaaca aaggtattat cgaattagag gtggtgtctc
     1561 taatggaagt ccttctccaa cacattttat cagacaccac acgcaaactg ttttctctgt
     1621 tttctccctc cctgtgtgtc acaatttatc aaatgcgcac atttacgaga tatattatgg
     1681 aagaatgaag aatgcgcgta catttttacg acgccgagaa aagttggtat atctcataat
     1741 cttgcaacga tgtactgaaa aattgggaac aaacaactaa taacaaacta ataaaaagtt
     1801 gtcgtgaatt ctacaaaatg tcatattgca ccatgttatt ttaaaattgt ttagtagtgt
     1861 caaaatatag aaaatcactt tcagttgaaa taaatgttat tttcagataa atcgagtgat
     1921 tttctatatt ttaaccctaa cgaacaattt tggaataaca tggtgcaaca tgacatttag
     1981 tagaattcat ggcaactttt attagtttga tcctaatttt tctatatttt ggaactgatt
     2041 ttcaattttt aaaaaaactt tggagcaaca tggcgcatcc ctggggtttt ctcatcagct
     2101 aactgtcacc tgttgtgttg ctcttcatac gattgcgcaa cttcttccgt ctgttgctag
     2161 tgaccccttg tcatattttg atggtccagc agcacacata ccagaagtcc tcataaatgt
     2221 gtaaacattt attcttcagg tggtgagtga cattgacact gactcacacc tcacacacac
     2281 acacacaaca gtttgccaac tcatttgaca cctatttgag agggggtgag gtacacgtgt
     2341 gtgaggggat ttgtttgccc gagttcttga gatgtggtgg tgccaaaaca atcaagaagc
     2401 tcttctctct ctcgtgtttc acacctctat ccatacagat tcggaggagg aagactgtct
     2461 caaaaaccga ccacttaaga gtgtgtgtgt gtgtgtgtgt gtgtgtggaa actgtgtgaa
     2521 caatcgcact tcaaaaacca tgaccatata tgggatggac tgattgattt gaatggatga
     2581 aaaaccgctt atcgtcgtgg attctcaaga gtcgttctct ggttctcagg attctctgga
     2641 tcaaaatagc aaaaagcagt gaactgttgg gatttttagg agtcaaagtg tatgtcacaa
     2701 atcatccaaa tcgcctctac tcttccaaaa acatgtcccg tcagtcaact cgtcaagata
     2761 tgcaaatttc aatatctgcc ttcatccata tctctttttg tctttcaatt ggtccctcgg
     2821 gggcatccaa gagacacgaa acatggggca acacgacttt gacaaggtta caacaacaca
     2881 atgcacgcgc taatcatatg tagagaagcg tctcattttg gtatgcgtat ccctctcacg
     2941 aaaccccgaa attagtggag atgatggtgt ctctctggcc gaatcataat gatggctatg
     3001 catagttgat tgcataacca taaggaatta caacaagaat tacaacaaga aaaagatgat
     3061 tttcgaacaa accttgacat ttaaaagtga aagacaaatt ccttgctctt cgaatgcaat
     3121 tctaatcgtt ttcgtgtccg agtcgatttc cattccaaga gtttcagtcg tcatatttga
     3181 attacttcga gccaaccgtc caatattctt ccaattatcg atatcctccg aaaatcgttc
     3241 cttctcgatt tttgtagatc cgggtcgaga cgttttgaat tgtttcaggt acacggagaa
     3301 tgtttcttta caggatttgg gattcaagta ggcatcacag tcacgtgtgt tgaagagcag
     3361 ttcgatgtag attctggaaa cgatgaaatt gattttttaa acactttttt gaaaaaaaat
     3421 tgacattgta aaaaataata tttcttctca aaactgtgaa ttattataat ttttgtgcac
     3481 tcgaattttg taaaaattgc ctttttgaaa gtacactacg gatttcgttt ttttgttgaa
     3541 aatttagaaa aactgaattt tttaaaaagt tttttttgta aaaaatatcg aaaaattgaa
     3601 attttttgta aaaaataacg aaaaattgaa atttttgatt ttttctttga aaatatcgaa
     3661 aaattgattt tttttaaaac attttttgtt gaaaatctag aaaaattgat tttttttttc
     3721 gttttttgtt aaattttagt tttaaaaatt atttttttcg tttttttttg ttgaaaatac
     3781 cgaagaattg aaatttttgg ttttttttta attcgagttt aaaaaattga attttttctt
     3841 ttttttcgaa tttttcaaaa ctactgtata tttttcaatt ttttgtcttt ttggaaaatt
     3901 ttactgcatt ttttctcgat tttccgatga tcatttttca ataatttttt tttcgatttt
     3961 caaaaaaaaa atcgaaatat aacaaatttt tttaaaaatt ttgattttca aattttctca
     4021 gaaaaatacc aaaaaatcga acattttaag ttttttttat ttttattttt aatatagctt
     4081 tcatttttct agaatttttg gagcatttct agttttttca aattcaatct ttcattaaaa
     4141 atttcagaaa aaattacctc ctcgcagttt taacctcgat aaaatgactg aacaaccaat
     4201 tgctcggatt tatcatatca taattacacg tgacgtaggc tctctgattg gcgtgcttct
     4261 cgtctgtcgc cgctggattt cgccaagttt cttccatcca ctgaaaattg atcatattcg
     4321 tttatacatt ttctgatgcc ttttccccgt caaataatga ataaattccc gcaattaatt
     4381 agtgaactgt gtttattcct tgtcatgtca catcaaggat taatatggta tttacaagtg
     4441 taaatgccct tatcttcgcc tctcgatctc tctaatccat ctctattagt tagtactaca
     4501 accaccactt tgcatcagca tcagcagtag cttctactat tcccgctaga aaatgaataa
     4561 aacaatcaat tccttcatcg actcgttcat gcagcaacaa taatggaaac atttgcattt
     4621 acatttggaa tgcataacaa aaccaatgtc atttgcagtg tagaagagtc atcacctttt
     4681 tgtgtacttt tgcttctttt agtgagtata ctactttact agtaactaca tatatatata
     4741 ggtcaaatgt cataatcaaa acatatggtg gaggagcaac acagacggtt tgaccggaag
     4801 aaggagagag cgtatgtcgt aatgtgtcca tgcatatcat gttgcgtcat accgtaacct
     4861 gccacatcat catcatcatc attgtttatt aatataaact aacattaagg aaagagtagc
     4921 gtttttgatt aagcataata aaactgggtg aatttcctaa tgggaattct cgaaaaaatt
     4981 ggtaaattcc aatttttggt agattccatt attttggtaa attaactcag tttaatcatc
     5041 tgcgaagttg ccgaaattcc agatttgagc aaaatttggc actttttttt ttgaaattct
     5101 gattaaagtc tgagtttcag tacatctaag caaaaacttc gaaccgttag ttctccttta
     5161 atgttgtaga tcccttccaa atttccaaga agagcctgct ttctgattat tgcgcaacag
     5221 cacgctaggc tccacttttg gaactttttg aacaatggaa ggaccccacc accaactttt
     5281 cattatatcc acactctggc cggcttcagt cttcctaaaa gaggcagacg cacacacgca
     5341 atgatgacta cgtctttttg ataaacatct tgtgtacaac ttcttcactc gcttatttta
     5401 gatggacact cacacagaca cgggtggtcc attctctctg gaatgccaaa ataatccccg
     5461 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgcatc tactcatttt atatggaact
     5521 tctcctagta gttgtacata tgtatggaga cgtagctgtc cttgaaaagt tttgaaataa
     5581 agtgtacaag ttgcattgag aagactatcc tctccgatga tgaggatgat gcacacatct
     5641 ttttctgcat tctcgctatt tctcgaacac acggagagac acacaatacc agggtgtgac
     5701 ctcctctcaa aaaaccattc aacggtgaag acactgcgtg ttctgagttg cctacttact
     5761 cttttctaaa cgtgtagtaa agaagatggg tgaacaagag aagattagga cacctcttcg
     5821 cgttttcaag tggtgtcaca ttgagcagtt ctttgggaaa acgaaacaag tcccttgaaa
     5881 acatcatcgt cgtcggatcg tccgtctccc tagtccgtga gagagtttac cgcgcggatt
     5941 agcataagac aaagtgtgcc ttctcctcct ctcttcgcgc attcagccat tttccatgat
     6001 gtttgttttc ccttcttctc actcactggg acacaggcat gagaaaatgc tcacttgtca
     6061 atcagctcat cattttcatc gtcaaagagt agttagttcc gtttagtagt ggatgggatg
     6121 agtgaaggag gggaagaagc tcataagttc gaagagcccg cttgatttat gtcctctaat
     6181 ttgtcaccac acaatgctga agacatcatc atcatcatca tcttcttcga gttaaacgcg
     6241 accacaaaac ctcacattat ttttgatttt ccgttttttt tctaaaaaaa agtgacaaaa
     6301 gcgacaaagt acatgtacag gaaccagttc ttctcttcca gtgtttctgt ttcaatacaa
     6361 cgccacataa tcataatatt aataataata tgtactattc catgcatcca gaagctaacg
     6421 gaacgagttt ctacagccgg cccggccagg tggtgtatct cttatgtaat ttcttttgtc
     6481 gtcaaagatg atggcgatac agtgacgagt agaatagtag aatacacctg cgtgtttaag
     6541 tcaagtcaca actaggtgat atgcagtcac acctgcctag taggcgccca gggtccatta
     6601 cccattaata caattgtgac atataacaat ccattattca acccgagggg gggggggggc
     6661 tccagaccgc gcatttgtca ctctggtcga gtcaactgcc accgagtctg aatagttgaa
     6721 tcagcaacaa caacaacaag cagaagcagc agcataatgc tccgaaggat atgggtgaaa
     6781 aatttgcatt tcaaatgctc cccccgaatc ttttcgtgga ttattagcca tctcacacct
     6841 gttgtctatt ggttgcacac tgagataacg catgaattga ttctatattg ttaattaata
     6901 gagagccaca cacttgactg agcactagtg gagcctccgg aatttcggac acattctaaa
     6961 ttacaggcta ccatgggtcc ctaatggatg cgcgcgtcaa atgacagggt cattatcact
     7021 tctacgaaaa gagagtacaa ggtcagccat aaaaacactg tgtagagagg tgttcaaaca
     7081 gtgacaagtt tcacatgttg caatttaccg cttcacttgg taggtataac ggaggggccc
     7141 ttttcttcac tattcaaaca acacttgtcg tcgtcgtcat tgaaaagaaa ttggtgatgt
     7201 gatgacgact ccggatgttt catcttgttg cagagagggt ccttcggaga gaaacagtat
     7261 aacatataac atccgctggc aggcatattg aggcgtaggt ctaggtcact taggactttg
     7321 ccaaaattca tgcctttctc tttcgtcttt ttcgattact agtaaattta caacccaatt
     7381 taggagtcag aagagctctc tacgaaacat atggacatgt tcttaataga ctttagctct
     7441 attttctatt cccccctccc tcctccatct cctctcatct acatcttcct acaccaatat
     7501 caacaccgtc tctcgactga ctaattgaat atgtccgagc gtgtgtgcgt cttcctagta
     7561 aagtgtatgg gaggaagaga agaaatgcaa aaatgaaacg aattcaattc aggccaacac
     7621 cgttaatggg attattttgt gtgttcagtt ttcctagaga agataaggga gttgaagttc
     7681 aaaatttcaa actaaaaatt ctgaagaaga ataaaaccgt cccaaaagta tgtctagtct
     7741 agcaaagtct agcacagggt taatagcccc cttttccttc ccaacggccg ttcttcaatg
     7801 ttaatgtcaa ctaataactg tacaatgtca caaattacta tgtagcacta tgtagtgacc
     7861 ttccatcaac tattcccgga tgtgctcata tttctgtccc ccttttcaaa aagttgttag
     7921 agatgggcaa aacctcctcc catcccaact aaaccaatgt gcttctcgtt tcaccctcat
     7981 cagatgtata tgcacaaaaa agccgctatg cttctttttc tttttcttct tccacaaaaa
     8041 ttcagaaaaa ttccaaattc ccatgggtca ctcactacta aaagagggag taggaaacac
     8101 aacaacttgt ttctttgcaa ttttgccgcc gccgacccca gcgagcgcat ctttttccta
     8161 catcttttca atttcaaacc aattcaagtg tactctagaa gattggaagg agactcacca
     8221 catcgtctcg atcgtgtcgc agcgagactt ggtcccattt tagatccgat ccaactttgg
     8281 acaagtcgaa gagaacttct gaaaaaaaaa atacttattt tgcatttctg ttcccactat
     8341 tctccaattt cacaaatgtt tttcgcccac aaatttttcc gaggcaggat atatatatgc
     8401 atgctgatgt ctcaaataca aacagcgccc cggggtctac ggggaggcca acacacacag
     8461 gaaaaaaact tcaatgatct caagatgttc ggaaattcgg aaatgaatag ggaggaatgg
     8521 ccacttgaca ctgtattcat gcaatgtcca tcaatacact caagcgccgg gataactgtt
     8581 ttttttgtct ggttggcaat ctcaatttct aggacgcccc ctgcttcctc actaataaaa
     8641 atatctccgc tttgaggtgg gggaggagga ggtgataaac tatttgctca tttttcgcgc
     8701 gcaaattttg ttttgttttt tttttctcgc gcccggaatc cgcttaattt gcttggcggc
     8761 tgggctgggc tctaagagtt cgtgacaatt tatcaggtag aacccgtgcg gaaagaagcc
     8821 taactccctc actttttatt catccaaaat gatagacttt tcctgaactc agcgaaccac
     8881 acgcacacac caaaagctag tactaattga ataggcacca cgtcgtcaaa taaaaaacgc
     8941 gaaagggtac taatcgccgg aagagggttt caaaaggact tcacacttaa aacggccctc
     9001 caaaaaaaaa ggacgcatgt ttcccgtgat ttattatgtt aaaagtggcg gtggtggggt
     9061 gactaaacgg tgcatgcatc gacgataagg atctctgggc gccaggcgcg cgggggttcc
     9121 catttccaaa aggggaaccg atttgtaaaa gatttgtatc tctggaaggc gcaagggaag
     9181 gagacgaggt ttacaagtca tcaatattga ccgggtctac ggtaggaatt cgagagtacc
     9241 ccgggaccct gtcagcttct gctcaaaggt gtcgtgtcaa tcagaacagt agggtaatcc
     9301 gagtaggatg tccgcgtaca ccactcaaaa acctctctcc ccctctcagg cgacacctat
     9361 cccgtataaa caaattggtc cgggggcttc ttctctaggc agttgccggg aggaagtgtt
     9421 ctcaaatgtc aatgtgtggt tcgaaaaata ataaagagag actcttgctc gtcttgttct
     9481 caattattca acatctcatt gtcgagattt tcaattttca attttcaatt tttcaattca
     9541 aagagagctc cgccgccgcc gcttgaacgc tcatttatct tttcccattg aaatctgtga
     9601 attcttcaat tgaggggtct aaaggtgcga ttcttgtcgc ttcagctccc gactgagctc
     9661 cgtgtgagaa acagttgttc aggaatatcc aatatctctc tttgcttatc acacctcgca
     9721 aatgatctat tattctaggc aggagcagca gaagaagcat aggaaagtaa aagctacttg
     9781 caaattgacc ggtggtcccc taaattctaa attaattgct accaaaaagg cgccgtggtg
     9841 tggcgccaca aagtcgctgc gtatcggctg gcttgaaatt agaatagtct caattggaat
     9901 agtaatcgaa gccaattcaa aacgcaattt gaaccagcaa ctagatcata gagagaaaga
     9961 aagaattcaa actacctaat acagtgtcac acatacagcc tgctctttac tactgcgcga
    10021 accggtttca caccaaggac gtggtacctg agcaagttcc caaaccacac cgtacaaaga
    10081 gacatgtacc ccaaaacaaa agtgttaatc tattcacgaa aaggaagagg ggactgtgtg
    10141 tgtgtgtgcg caaacaagta cgtatataca tcaatacaca gggattgctc tttttctttt
    10201 tttgactgtg aggtaaaata ttttttctct gacttacctt gatgtgatgt gacgattgtg
    10261 atcagacact gtagaaccag aataaatatc gattggtggg gatttagaat ccgcgaattg
    10321 tacaaccgca ttttcttgaa aaatgggggt tcttttgaaa atggttagtg gatttagtga
    10381 cgcgagtttt gatgcatttg aggtgtgtcg tttttttggg ctgaaaattg gtgaaaatta
    10441 agaaaaaaaa atgaaaaaaa aaagacattt tcagaaatta agagcaaaga aaaaaaaggt
    10501 gatttggaga cgagaattgg gaaaaattcc aaaaagactt tcgccgaatt ttttgttttg
    10561 ttttttgtta aaaaagaaga aaattatatt caaaaattcc aaacttatat aaaaaatcca
    10621 aaaaaaagga aaatttgtga tatttttaaa aaaatcaaaa aaaaaagtta tttaattata
    10681 aaaatgaatt ttcagtggag tttatacgaa aaatgaatca ttttttgaaa attttcttca
    10741 aaattttttt ttttaatttt ataaagtttg aaaatgttta aattacaatt ttcaaatttc
    10801 aaaaatttca cttttttaca gtaattgaac tcggcgaaaa attttattaa ttttttttat
    10861 ttgaaatggt ggtacagaat tgtgggaagg aatttttcaa aaaaaaaatt taaaataggg
    10921 taatttttgc aaaaccagta caagcaaaaa aattgggaag taataataaa tgatgaaaaa
    10981 tgaattccag aaaaaagttg agaaatagag aaaaaaggaa aagctcttct gtgggtcaac
    11041 cggacactct cttctcagat gcacccccca gcagaaacca agaaagatag gcctttgggg
    11101 ggcggggcag gtggtcgtag tgaaggcccc gccccccgat cgggcggcag agggcaaaac
    11161 atatgcgaga gcggcggcgg tgatctactt gtgggaggac gagaagtgcg ccaactatta
    11221 gaaactgtag atcatatgaa gaagaagaat tgaatccact tagttgtgtt ctaacaggga
    11281 tgaatccaga agaagaagaa gaagtcacta tgatttgatt attggaacat gaaagaagga
    11341 gacaaaagac aagatgccca tttacatagc taatctggtt taatttataa ccgtttcaaa
    11401 tttttatgat ttagcagaaa gagaaaaatt attcggaaaa ggtactgtaa ctccgggtgc
    11461 tggtaaactt tttgatacaa ttaattatga ctaaaactga aaattgggta actgaataag
    11521 tagatataat tttgataaaa cattaccaat tagttgagaa atatatttct aatctgtcaa
    11581 ttgccatttt tctttcattt tcggcaattt gcctatttgc cggaaatttt cattttcggc
    11641 acattgccga tttgtcggaa ttttcatttt cgacaatttg ccgatttgtc ggaattttca
    11701 ttttcgacaa tttaccgatg tgccggaaat tttcattgtt ggacatttta ccgatgtgcc
    11761 ggaaattttc agtttcggca gtttgccgat ttgccggaaa ttttcattat cggcattttg
    11821 ccggaaattt tccttttcgg caaattgccg gtttgtcgga aaattttcat ttctggcaaa
    11881 ttggcgattt gccggaaatt ttcattgttg gacaatttgc cggtttgccg ggaattttaa
    11941 ttttcggcaa tttgccgatt tgccgggaat tttctttttc ggcaatatgc cgatttgccg
    12001 aattttcatt gggattcttt ccatttttca taggtttctt taaattttat agaaaatcat
    12061 gtggaaatgt ataaaacata caaaaaactt tatttaaaaa actagacaaa attgaagaaa
    12121 atcccacaac tacagtactc cttcaacgca cacgctcgtt tgaattcaag aaaatctgtc
    12181 gattgcacca tgtcagagag ggtgagaacc ctattatctt aaactgccag cctcactaat
    12241 cgtatcgaaa aatctcgtgg caacatgatt tacaatgata acctcaacct caagatgggg
    12301 gtgttgcgaa acaaacacgg ataaccacaa cagatttacg acccggtcgt aactttgtaa
    12361 cccgcgtgcg agaagaatat tagaatggat agattttcca taatattcag gatgaaccta
    12421 gaaagataat tataataatc agcgaaaacg ctacgcccat cccaataaag ttatgtaacc
    12481 tgtgtgatga atagacgaag tcggggggtg atttgcgact gactcaagtg gtggtggaga
    12541 cacacaaaaa tgagggaaaa tggagaacaa ctagttgtaa tttggaacac cgcgtacctc
    12601 ccgatcacaa aaatagatac ttcttcttga caatcgagcg tgtgaaaaac accttttttc
    12661 gtccattttt ctccacaatt gaccactatg aagaaagcat gactatacaa gggggaatca
    12721 tcatcatcat catcatcatc aaaaatgttt tatatagttg ttgataagtt tgtgtgcttt
    12781 cgtcgtgtcg gtccccggtg ctttgatgtt ttacgcaata gtatatggtc gagaatgggc
    12841 aaagaaccta atttattggt gtacttggag acaagaacct tttggggcta gtcatcgtgt
    12901 tgattaggtt aagggtatct atcgatcaac acaaaacatt tatgagacta attaataaaa
    12961 ctggggatag tagatgctag taggtttctg tgagacaatt gtcaatttga aggtattcga
    13021 atatttcagt tggtaaattg ctaacttttg cgaatttcta aaagaattta tttgattagt
    13081 ttagtaagtt ctctcaaata tttttcctca ttatttattg atagaatata ggggtgggcg
    13141 ggaattgaaa atatccggca aattggcaaa ctgccggaat taaaaatttc cggcaaatcg
    13201 gtaatttgcc gaaaatgaaa ttttccggga aaccggtaaa ttgccggaat tgaaattttc
    13261 cggcaaatcg gtaatttgcc gaaaatgaaa ttttctggca aatcagaaat ttgccggaat
    13321 cgaatatttc aggaaaatcc gtgattttcc gaaaattaaa ttttccggca aaccggcaat
    13381 tttccgaaaa ttgaaaattc cggcaaattg tcgatttgtc gaacttgtcg gcaacatatt
    13441 cgccgccctt ccaaccaggg gcggcaattg acgctcggca aattctgcaa atcgccaaat
    13501 tgccagaaat tttaattttc gacaaattgc cggtttgcca atttgccgga aatttttaga
    13561 gggctttttt ataagacaag cacacttaaa cagtgtattt tgaaaaattt tcccgttttc
    13621 tttagatatt ttcatagaat ttactaactt ttcaaaatag atgtaggaaa attcatagga
    13681 taagtacaat tttgtgaaat tttttggaaa aattcgtagc tttttagagt aaaataaact
    13741 agtaaatcac taatattcta ttcaaaagaa cacagaaaat cgatggctaa atgcctgaaa
    13801 gaaattcgaa ttccagtccg atttaaattt ctcgaaagaa aagacgattg gtgaatgttt
    13861 aatgaaacaa acaaagagaa taagtaggta ggcaaaaaag tagaaagaag ggaataataa
    13921 cgagtcattc agacaacctg tcttacggga agcagagtaa gagagaccct tcctaactgt
    13981 ctcaatctca tgtgctaata atcgtggaaa cgtagtgggt gcaaaggacg agtatcattg
    14041 tcttattgct cttctctttt tcatgttctc acccaatttt ccgatagaga gacggctaat
    14101 tgcctttccc acagtatgta tctctaggga agacaccacc gatatggaga gcactgttgt
    14161 ttggttaggc tcctactggg aagaagaaga aaaaagagcc cgccgactaa ccaacaataa
    14221 caacatgacg aaaaagaaga agaatggata tccgctcgta tccggaggat acaggcattc
    14281 cctgctatta gcttatgctg ttgaaaacat gaatattcgg aatttttacg attttgaaaa
    14341 gttgaaaatc ggcaaaaatg cagctaatca gggctgggac ttttttggtt tttttttgtt
    14401 ttttggtatt ttgacgaaaa atcgtttttt tttttggttt tttggtacca aaaaaccaaa
    14461 aaaattaaaa aaaaattttg ccgtgtcgag tctcgactcg acgtcccatg gccattcgaa
    14521 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
    14581 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
    14641 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct
    14701 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
    14761 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
    14821 gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
    14881 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
    14941 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
    15001 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
    15061 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
    15121 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
    15181 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
    15241 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
    15301 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
    15361 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
    15421 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
    15481 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
    15541 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
    15601 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
    15661 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
    15721 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
    15781 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
    15841 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
    15901 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
    15961 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
    16021 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
    16081 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
    16141 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
    16201 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
    16261 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
    16321 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
    16381 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
    16441 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
    16501 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    16561 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    16621 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    16681 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    16741 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    16801 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    16861 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    16921 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    16981 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    17041 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    17101 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    17161 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    17221 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    17281 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    17341 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    17401 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
    17461 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
    17521 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
    17581 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcttttcctc
    17641 ttttttttct ccttctctcc ttcaactagt tgttgtataa tatgtatagc ttttccctct
    17701 gaaaatttca gatttttttc tgtgatagtc gcagtctgtc tcttttttcc ggtttctctc
    17761 ttcatccaac acttccagcc aaacctcttt atattttgta ttccggtgcg ttttagcaac
    17821 taactatccc aattttcctc caaaaaaaaa cccaccaagt catattatgt atagcacttt
    17881 atatatatcg tattgctttt ttcccaatta ctggggataa ataagtacgg tatcaggttt
    17941 aattaaattt tattttcaag caatttgttc caagtttgcc aaaaaaaacg aacaagaaat
    18001 cgcccgcaaa acacagtgag cagggtatca cagaaaatga tgggagaggg ggaaaatgcg
    18061 caaaaaaatt gaaaaagtgt gtgtgtgtac cagtaaaact ttctgaaaag caatgtcagg
    18121 agggtggagg cttacaaatc gttgctaaaa tactaaattt gttgagtaga tcatatcatc
    18181 ggggagaggg gaacattagg gggaaaaatg cagaaaaaaa ggatttaaag acacagagga
    18241 aaacataata tcgaatcggc tacccataac agttttgaaa atgcacatat catcggattg
    18301 attttggaat ttttttgggg gaaagaaaaa cgaaatttac acatacaaac agtcctaaaa
    18361 aggttttgaa acaactagga atcagatgct tctaaacaaa gaatccctct tctcgaggcg
    18421 gtctcgtcgt tcttgttcca cgtgtcgtcg cgtggattgt tccagaagag cccggtggtg
    18481 agccactgat gcgtccgttg agtccatccc gaacggtcga gcactccgcg gacatgagac
    18541 cataggcaag aagatccaag tgagacgtgt tggccaagtc actcacagag tgaatgttat
    18601 tcgacacgag ttgtccgtag acatgattta ggccaatttg acggagaaaa tcgtcgagcg
    18661 atggcatcgg agcagccgac gatggtgggg ttggaactac tccgacattc acagatccat
    18721 aggtattcag ggcagattga ctctgaaccc gtcgcggaag ctctccagga tcatgctcaa
    18781 taagtgtcgg ctgcaggatg tacttgtgga atgtggcgag aagctgtgtg aacgtcggac
    18841 gctcgtgtcg ctccatcttc caacaccact gggcgatccg atacaatccc atcggacaat
    18901 ccatcggtgg tggtagtcga tagccgatca tcacttcgct gatcaccttc tgattcgtcc
    18961 aatcccaata tggtcgttca ccgaatgaac acacttccca gatcacaact ccgaacgacc
    19021 agacatcaga ggatggagtg aactttcgat gggtaattgc ttctggagca gtccatctga
    19081 ctggaatctt tccgccattt gtagtgtatt cgggttcgac agaagatcct tcacttctga
    19141 caccacggga aagtccaaaa tcagcgattt tgcagcgaag ctccatgtcg agaagaacgt
    19201 ttcgggcggc gagatctctg tgcacatatc ccatatctgt caaatacttc attccagaag
    19261 caattccata caacatttca gtgatcttct cccaatgaac cttctctcct cgttgatccg
    19321 tattccgaag gaattgatcc aaagatccat ttgccatgta ctcggccaca atcatcactg
    19381 gctccgctga cgtcacaact ccaatgagcc gaatcacatt tggatgtgag aattgtccca
    19441 tgatggttgc ttccgtcaaa aattcggctt tcgctttcgc actgcttcca gatttcagag
    19501 ttttgatggc aaccgtctca tatggttctg attcgtatcc tccaccacct cctcctcgtc
    19561 ccattgttgt atttgaaaca ctccatttcg cggccgcagg cgagtttttc gacagaccac
    19621 ccaggcagac atcgccgaat tctcctccac caataacctg agtaatgaag acgtcgtttg
    19681 gtgaaatgtc gaatgtgaat tcgatgagtg cttgatatgg atcttcatat gttgttggat
    19741 ccacatatgt cttatatttt ccactattcg gatgtactcc accgtagtat ggtggtggtt
    19801 gggagattgg agatccaaat gatggaatta gtggagcatt caactttgta gtcgatcgaa
    19861 gttgttcgtg cagagtagcc ggtagatttc cctgatgatg atgatgtcga gatgttgtat
    19921 gatagtcagg tgtcattgaa tcttgtttat aagtgtccaa aacatccaaa tcgctcatct
    19981 gtttccgatt cttcgacttt ttctgcacca caatcataca aagagcaact gcaatgacaa
    20041 taagtatcaa agctataatg attaaaagtg cagatccagt ttgattcaaa aactgagatt
    20101 cttcgacttc attcgacgac ggagagctca tcaatcctcg tccaagttga taactgatcg
    20161 gttgactcca acgaagatca tctcgaattc gtactcggaa ttgatatacc gtttctggat
    20221 ttaatccaat aaaagtggta tatgtcatgt taacatgtcg agtttcgaaa gttctcttct
    20281 tcacaattgc cggacgaact tccacttcaa aatccgaaac atcagaatcc gaaacactcc
    20341 aggcgatggt gattccatcg gattgagaag catcaactct taaatgtgac acaggtggaa
    20401 catcttcctc ttctgatcgt gttctgatat caataaccgc attcttctca ctaaactcgg
    20461 gaaactgagc agacacctta ttcttggcga acaccaaaaa agtgtagtcg gatgatggtc
    20521 tgagcccatt gatttgcaca gatcttgtcg atagtttctt atcaccaggt gtcataacta
    20581 ctgttccaca ctctcctcgt cccgaacatt tcacttcata ccaaatttct gggcgacctc
    20641 ccagactgct cggctcattc caagagagac gggtcgacgt ggcggtgatc gcgttggcca
    20701 ctagttttat cggtcgggat ggtggttgag tgcatggcat cgaaatgagc tcatcttctg
    20761 ctctgaaata accattaaga cagcgacaag agctggcagc ttccgaagat atcgaattgg
    20821 atggacacga tttgcaaagg gattggcctt ttggtttgta cgtgttcgtt ggacagctct
    20881 cacaccgagc tccattcgaa tcggcaattt gagaatatcc actatcacaa acgcactcgc
    20941 cagacgtttt gataccactt ccagtcgaca tacacaatcc gatcaaatcg actcccggga
    21001 tctttttcga cgcattcgga atacacgaac cattcattcg gaccatatca gtctcttttg
    21061 gcccggtaac ttgtggtcgg aagtagacaa gttgatcggt gaattcgtcg catattcggt
    21121 agtaaatctg aaaataaatg gaattaaaat ggggtattaa aaggcatgtt aggctgatg
//
