LOCUS       sequence-256363-        9167 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Piggybac vector for constitutive expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9167)
  AUTHORS   Fischer AD, Veronese-Paniagua DA, Swaminathan S, Kashima H, Rubin 
            DC, Madison BB
  TITLE     The oncogenic function of PLAGL2 is mediated via ASCL2 and IGF2 and 
            a Wnt-independent mechanism in colorectal cancer.
  JOURNAL   Am J Physiol Gastrointest Liver Physiol. 2023 Jun 13. doi: 
            10.1152/ajpgi.00058.2022.
  PUBMED    37310750
REFERENCE   2  (bases 1 to 9167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J 
            Physiol Gastrointest Liver Physiol. 2023 Jun 13. doi: 
            10.1152/ajpgi.00058.2022."
COMMENT     Sequence Label: BII-ChBtW-CTNNB1_S33Y
FEATURES             Location/Qualifiers
     source          1..9167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..39
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(308..324)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(308..324)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      1257..1266
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1263..1658
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             1677..1730
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1740..1763
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1776..4121
                     /codon_start=1
                     /gene="CTNNB1"
                     /product="protein involved both in cell-cell adhesion as 
                     part of the cadherin protein complex, and in gene 
                     transcription as part of the Wnt signaling pathway"
                     /label=CTNNB1
                     /note="also known as catenin beta-1; related to Drosophila 
                     armadillo"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDYGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     misc_feature    4144..4732
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4197..4217)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4615..4626)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4812..4946
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4836..4855)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4890..4909
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   5724..5786
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5916..5933)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5916..5932)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5925..5947)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6074..6529
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6161..6180)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6371..6392
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6555..6659
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6660..6728,6729..7520)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6878..6897)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7691..8279
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8180..8199
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8433..8450
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8567..8588
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8603..8608,8609..8626,8627..8633)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8641..8657
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8646..8668
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8665..8681
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8665..8681
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat ccactagttc tagagggaca gccccccccc aaagccccca gggatgtaat
      361 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
      421 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
      481 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
      541 ggatacgggg aaaaggcctc caaggcctgc aggagtcaat gggaaaaacc cattggagcc
      601 aagtacactg actcaatagg gactttccat tgggttttgc ccagtacata aggtcaatag
      661 ggggtgagtc aacaggaaag tcccattgga gccaagtaca ttgagtcaat agggactttc
      721 caatgggttt tgcccagtac ataaggtcaa tgggaggtaa gccaatgggt ttttcccatt
      781 actgacatgt atactgagtc attagggact ttccaatggg ttttgcccag tacataaggt
      841 caataggggt gaatcaacag gaaagtccca ttggagccaa gtacactgag tcaataggga
      901 ctttccattg ggttttgccc agtacaaaag gtcaataggg ggtgagtcaa tgggtttttc
      961 ccattattgg cacatacata aggtcaatag gggtgactag tggagaagag catgcttgag
     1021 ggctgagtgc ccctcagtgg gcagagagca catggcccac agtccctgag aagttggggg
     1081 gaggggtggg caattgaact ggtgcctaga gaaggtgggg cttgggtaaa ctgggaaagt
     1141 gatgtggtgt actggctcca cctttttccc cagggtgggg gagaaccata tataagtgca
     1201 gtagtctctg tgaacattcg gtttagtgaa cctgcaggtc ggccgccacg accggtgcca
     1261 ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa
     1321 tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg
     1381 gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg
     1441 tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg
     1501 gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc
     1561 tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga
     1621 ttcgtgaatt gctgccctct ggttatgtgt gggagggcgc tagccttggc tccggcgagg
     1681 gcaggggaag tcttctaaca tgcggggacg tggaggaaaa tcccggccca ctcggcatgg
     1741 actacaagga cgacgatgac aagctcgata gcggcatggc tactcaagct gatttgatgg
     1801 agttggacat ggccatggaa ccagacagaa aagcggctgt tagtcactgg cagcaacagt
     1861 cttacctgga ctatggaatc cattctggtg ccactaccac agctccttct ctgagtggta
     1921 aaggcaatcc tgaggaagag gatgtggata cctcccaagt cctgtatgag tgggaacagg
     1981 gattttctca gtccttcact caagaacaag tagctgatat tgatggacag tatgcaatga
     2041 ctcgagctca gagggtacga gctgctatgt tccctgagac attagatgag ggcatgcaga
     2101 tcccatctac acagtttgat gctgctcatc ccactaatgt ccagcgtttg gctgaaccat
     2161 cacagatgct gaaacatgca gttgtaaact tgattaacta tcaagatgat gcagaacttg
     2221 ccacacgtgc aatccctgaa ctgacaaaac tgctaaatga cgaggaccag gtggtggtta
     2281 ataaggctgc agttatggtc catcagcttt ctaaaaagga agcttccaga cacgctatca
     2341 tgcgttctcc tcagatggtg tctgctattg tacgtaccat gcagaataca aatgatgtag
     2401 aaacagctcg ttgtaccgct gggaccttgc ataacctttc ccatcatcgt gagggcttac
     2461 tggccatctt taagtctgga ggcattcctg ccctggtgaa aatgcttggt tcaccagtgg
     2521 attctgtgtt gttttatgcc attacaactc tccacaacct tttattacat caagaaggag
     2581 ctaaaatggc agtgcgttta gctggtgggc tgcagaaaat ggttgccttg ctcaacaaaa
     2641 caaatgttaa attcttggct attacgacag actgccttca aattttagct tatggcaacc
     2701 aagaaagcaa gctcatcata ctggctagtg gtggacccca agctttagta aatataatga
     2761 ggacctatac ttacgaaaaa ctactgtgga ccacaagcag agtgctgaag gtgctatctg
     2821 tctgctctag taataagccg gctattgtag aagctggtgg aatgcaagct ttaggacttc
     2881 acctgacaga tccaagtcaa cgtcttgttc agaactgtct ttggactctc aggaatcttt
     2941 cagatgctgc aactaaacag gaagggatgg aaggtctcct tgggactctt gttcagcttc
     3001 tgggttcaga tgatataaat gtggtcacct gtgcagctgg aattctttct aacctcactt
     3061 gcaataatta taagaacaag atgatggtct gccaagtggg tggtatagag gctcttgtgc
     3121 gtactgtcct tcgggctggt gacagggaag acatcactga gcctgccatc tgtgctcttc
     3181 gtcatctgac cagccgacac caagaagcag agatggccca gaatgcagtt cgccttcact
     3241 atggactacc agttgtggtt aagctcttac acccaccatc ccactggcct ctgataaagg
     3301 ctactgttgg attgattcga aatcttgccc tttgtcccgc aaatcatgca cctttgcgtg
     3361 agcagggtgc cattccacga ctagttcagt tgcttgttcg tgcacatcag gatacccagc
     3421 gccgtacgtc catgggtggg acacagcagc aatttgtgga gggggtccgc atggaagaaa
     3481 tagttgaagg ttgtaccgga gcccttcaca tcctagctcg ggatgttcac aaccgaattg
     3541 ttatcagagg actaaatacc attccattgt ttgtgcagct gctttattct cccattgaaa
     3601 acatccaaag agtagctgca ggggtcctct gtgaacttgc tcaggacaag gaagctgcag
     3661 aagctattga agctgaggga gccacagctc ctctgacaga gttacttcac tctaggaatg
     3721 aaggtgtggc gacatatgca gctgctgttt tgttccgaat gtctgaggac aagccacaag
     3781 attacaagaa acggctttca gttgagctga ccagctctct cttcagaaca gagccaatgg
     3841 cttggaatga gactgctgat cttggacttg atattggtgc ccagggagaa ccccttggat
     3901 atcgccagga tgatcctagc tatcgttctt ttcactctgg tggatatggc caggatgcct
     3961 tgggtatgga ccccatgatg gaacatgaga tgggtggcca ccaccctggt gctgactatc
     4021 cagttgatgg gctgccagat ctggggcatg cccaggacct catggatggg ctgcctccag
     4081 gtgacagcaa tcagctggcc tggtttgata ctgacctgta atgctagagt tcgcgtctgg
     4141 aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     4201 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     4261 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     4321 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     4381 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4441 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4501 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     4561 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     4621 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     4681 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     4741 aattctggct aagcacttcg tggccgtcga tcgtttaaag ggaggtagtg agtcgagata
     4801 tcgatactag tgatccagac atgataagat acattgatga gtttggacaa accacaacta
     4861 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     4921 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     4981 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     5041 ctgattatga tcctctagag ggacagcccc cccccaaagc ccccagggat gtaattacgt
     5101 ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt ccggcgctcc
     5161 ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg
     5221 gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc tggggggata
     5281 cggggaaaag ccataccaat ggagctcacg gggacagccc ccccccaaag cccccaggga
     5341 tgtaattacg tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg
     5401 tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga
     5461 aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac
     5521 ctggggggat acggggaaaa agaattcgat aaaagttttg ttactttata gaagaaattt
     5581 tgagtttttg ttttttttaa taaataaata aacataaata aattgtttgt tgaatttatt
     5641 attagtatgt aagtgtaaat ataataaaac ttaatatcta ttcaaattaa taaataaacc
     5701 tcgatataca gaccgataaa acacatgcgt caattttaca catgattatc tttaacgtac
     5761 gtcacaatat gattatcttt ctagggttaa tctagctgcg tgttctgcag cgtgtcgagc
     5821 atcttcatct gctccatcac gctgtaaaac acatttgcac cgcgagtctg cccgtcctcc
     5881 acgggttcaa aaacgtgaat gaacgaggcg cgctcactgg ccgtcgtttt acaacgtcgt
     5941 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     6001 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     6061 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     6121 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     6181 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     6241 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     6301 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     6361 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     6421 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6481 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     6541 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6601 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6661 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6721 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     6781 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6841 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6901 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6961 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     7021 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     7081 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     7141 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     7201 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     7261 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     7321 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     7381 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7441 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7501 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7561 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7621 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7681 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7741 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7801 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     7861 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7921 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7981 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     8041 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     8101 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     8161 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     8221 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     8281 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     8341 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8401 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8461 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     8521 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     8581 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     8641 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     8701 cccgccgggt aactcacggg gtatccatgt ccatttctgc ggcatccagc caggataccc
     8761 gtcctcgctg acgtaatatc ccagcgccgc accgctgtca ttaatctgca caccggcacg
     8821 gcagttccgg ctgtcgccgg tattgttcgg gttgctgatg cgcttcgggc tgaccatccg
     8881 gaactgtgtc cggaaaagcc gcgacgaact ggtatcccag gtggcctgaa cgaacagttc
     8941 accgttaaag gcgtgcatgg ccacaccttc ccgaatcatc atggtaaacg tgcgttttcg
     9001 ctcaacgtca atgcagcagc agtcatcctc ggcaaactct ttccatgccg cttcaacctc
     9061 gcgggaaaag gcacgggctt cttcctcccc gatgcccaga tagcgccagc ttgggcgatg
     9121 actgagccgg aaaaaagacc cgacgatatg atcctgatgc agctaga
//
