LOCUS       sequence-256366-        9866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Piggybac vector for constitutive expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9866)
  AUTHORS   Fischer AD, Veronese-Paniagua DA, Swaminathan S, Kashima H, Rubin 
            DC, Madison BB
  TITLE     The oncogenic function of PLAGL2 is mediated via ASCL2 and IGF2 and 
            a Wnt-independent mechanism in colorectal cancer.
  JOURNAL   Am J Physiol Gastrointest Liver Physiol. 2023 Jun 13. doi: 
            10.1152/ajpgi.00058.2022.
  PUBMED    37310750
REFERENCE   2  (bases 1 to 9866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J 
            Physiol Gastrointest Liver Physiol. 2023 Jun 13. doi: 
            10.1152/ajpgi.00058.2022."
COMMENT     Sequence Label: BII-ChBtW-dnTCF4-ires-GFP
FEATURES             Location/Qualifiers
     source          1..9866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..39
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(308..324)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(308..324)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      1257..1266
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1263..1658
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             1677..1730
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1740..1763
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1782..1805
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3487..4052
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3503..4054
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3647..3664)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3874..3893
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4066..4068,4069..4071,4072..4782)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4111..4132)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4372..4391)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4719..4740
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4786..4806
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    4843..5431
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4896..4916)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5314..5325)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5511..5645
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5535..5554)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5589..5608
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   6423..6485
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6615..6632)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6615..6631)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6624..6646)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6773..7228
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6860..6879)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7070..7091
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7254..7358
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7359..7427,7428..8219)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7577..7596)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8390..8978
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8879..8898
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9132..9149
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9266..9287
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9302..9307,9308..9325,9326..9332)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9340..9356
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9345..9367
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9364..9380
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9364..9380
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat ccactagttc tagagggaca gccccccccc aaagccccca gggatgtaat
      361 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
      421 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
      481 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
      541 ggatacgggg aaaaggcctc caaggcctgc aggagtcaat gggaaaaacc cattggagcc
      601 aagtacactg actcaatagg gactttccat tgggttttgc ccagtacata aggtcaatag
      661 ggggtgagtc aacaggaaag tcccattgga gccaagtaca ttgagtcaat agggactttc
      721 caatgggttt tgcccagtac ataaggtcaa tgggaggtaa gccaatgggt ttttcccatt
      781 actgacatgt atactgagtc attagggact ttccaatggg ttttgcccag tacataaggt
      841 caataggggt gaatcaacag gaaagtccca ttggagccaa gtacactgag tcaataggga
      901 ctttccattg ggttttgccc agtacaaaag gtcaataggg ggtgagtcaa tgggtttttc
      961 ccattattgg cacatacata aggtcaatag gggtgactag tggagaagag catgcttgag
     1021 ggctgagtgc ccctcagtgg gcagagagca catggcccac agtccctgag aagttggggg
     1081 gaggggtggg caattgaact ggtgcctaga gaaggtgggg cttgggtaaa ctgggaaagt
     1141 gatgtggtgt actggctcca cctttttccc cagggtgggg gagaaccata tataagtgca
     1201 gtagtctctg tgaacattcg gtttagtgaa cctgcaggtc ggccgccacg accggtgcca
     1261 ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa
     1321 tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg
     1381 gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg
     1441 tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg
     1501 gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc
     1561 tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga
     1621 ttcgtgaatt gctgccctct ggttatgtgt gggagggcgc tagccttggc tccggcgagg
     1681 gcaggggaag tcttctaaca tgcggggacg tggaggaaaa tcccggccca ctcggcatgg
     1741 actacaagga cgacgatgac aagctcgata gcggcatggc tgactacaag gacgacgatg
     1801 acaagtccat ggctgctgat gtcaaatcgt ctctagtcaa tgaatcagaa acgaatcaaa
     1861 acagctcctc cgattccgag gcggaaagac ggcctccgcc tcgctccgaa agtttccgag
     1921 acaaatcccg ggaaagtttg gaagaagcgg ccaagaggca agatggaggg ctctttaagg
     1981 ggccaccgta tcccggctac cccttcatca tgatccccga cctgacgagc ccctacctcc
     2041 ccaacggatc gctctcgccc accgcccgaa cctatctcca gatgaaatgg ccactgcttg
     2101 atgtccaggc agggagcctc cagagtagac aagccctcaa ggatgcccgg tccccatcac
     2161 cggcacacat tgtctctaac aaagtgccag tggtgcagca ccctcaccat gtccaccccc
     2221 tcacgcctct tatcacgtac agcaatgaac acttcacgcc gggaaaccca cctccacact
     2281 taccagccga cgtagacccc aaaacaggaa tcccacggcc tccgcaccct ccagatatat
     2341 ccccgtatta cccactatcg cctggcaccg taggacaaat cccccatccg ctaggatggt
     2401 tagtaccaca gcaaggtcaa ccagtgtacc caatcacgac aggaggattc agacacccct
     2461 accccacagc tctgaccgtc aatgcttcca tgtccaggtt ccctccccat atggtcccac
     2521 cacatcatac gctacacacg acgggcattc cgcatccggc catagtcaca ccaacagtca
     2581 aacaggaatc gtcccagagt gatgtcggct cactccatag ttcaaagcat caggactcca
     2641 aaaaggaaga agaaaagaag aagccccaca taaagaaacc tcttaatgca ttcatgttgt
     2701 atatgaagga aatgagagca aaggtcgtag ctgagtgcac gttgaaagaa agcgcggcca
     2761 tcaaccagat ccttgggcgg aggtggcatg cactgtccag agaagagcaa gcgaaatact
     2821 acgagctggc ccggaaggag cgacagcttc atatgcaact gtaccccggc tggtccgcgc
     2881 gggataacta tggaaagaag aagaagagga aaagggacaa gcagccggga gagaccaatg
     2941 aacacagcga atgtttccta aatccttgcc tttcacttcc tccgattaca gacctgagcg
     3001 ctcctaagaa atgccgagcg cgctttggcc ttgatcaaca gaataactgg tgcggccctt
     3061 gcaggagaaa aaaaaagtgc gttcgctaca tacaaggtga aggcagctgc ctcagcccac
     3121 cctcttcaga tggaagctta ctagattcgc ctcccccctc cccgaacctg ctaggctccc
     3181 ctccccgaga cgccaagtca cagactgagc agacccagcc tctgtcgctg tccctgaagc
     3241 ccgaccccct ggcccacctg tccatgatgc ctccgccacc cgccctcctg ctcgctgagg
     3301 ccacccacaa ggcctccgcc ctctgtccca acggggccct ggacctgccc ccagccgctt
     3361 tgcagcctgc cgccccctcc tcatcaattg cacagccgtc gacttcttcc ttacattccc
     3421 acagctccct ggccgggacc cagccccagc cgctgtcgct cgtcaccaag tctttagaat
     3481 aggaattccg cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     3541 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     3601 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     3661 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     3721 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     3781 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     3841 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     3901 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     3961 acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg aaccacgggg
     4021 acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggt gagcaagggc
     4081 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     4141 cacaagttca gcgtgtctgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     4201 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     4261 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     4321 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     4381 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     4441 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     4501 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggcgaac
     4561 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     4621 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     4681 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     4741 accgccgccg ggatcactct cggcatggac gagctgtaca agggccccaa gaagaagcgc
     4801 aaggtgggcg cctaagtaca agtaagagtt cgcgtctgga acaatcaacc tctggattac
     4861 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4921 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     4981 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     5041 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     5101 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     5161 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     5221 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     5281 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     5341 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     5401 agtcggatct ccctttgggc cgcctccccg cctggaatta attctggcta agcacttcgt
     5461 ggccgtcgat cgtttaaagg gaggtagtga gtcgagatat cgatactagt gatccagaca
     5521 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     5581 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     5641 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     5701 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cctctagagg
     5761 gacagccccc ccccaaagcc cccagggatg taattacgtc cctcccccgc tagggggcag
     5821 cagcgagccg cccggggctc cgctccggtc cggcgctccc cccgcatccc cgagccggca
     5881 gcgtgcgggg acagcccggg cacggggaag gtggcacggg atcgctttcc tctgaacgct
     5941 tctcgctgct ctttgagcct gcagacacct ggggggatac ggggaaaagc cataccaatg
     6001 gagctcacgg ggacagcccc cccccaaagc ccccagggat gtaattacgt ccctcccccg
     6061 ctagggggca gcagcgagcc gcccggggct ccgctccggt ccggcgctcc ccccgcatcc
     6121 ccgagccggc agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg gatcgctttc
     6181 ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc tggggggata cggggaaaaa
     6241 gaattcgata aaagttttgt tactttatag aagaaatttt gagtttttgt tttttttaat
     6301 aaataaataa acataaataa attgtttgtt gaatttatta ttagtatgta agtgtaaata
     6361 taataaaact taatatctat tcaaattaat aaataaacct cgatatacag accgataaaa
     6421 cacatgcgtc aattttacac atgattatct ttaacgtacg tcacaatatg attatctttc
     6481 tagggttaat ctagctgcgt gttctgcagc gtgtcgagca tcttcatctg ctccatcacg
     6541 ctgtaaaaca catttgcacc gcgagtctgc ccgtcctcca cgggttcaaa aacgtgaatg
     6601 aacgaggcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     6661 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     6721 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     6781 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6841 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6901 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6961 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     7021 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7081 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7141 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7201 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     7261 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7321 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7381 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7441 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7501 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7561 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7621 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7681 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7741 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7801 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7861 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7921 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7981 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8041 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8101 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8161 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8221 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8281 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8341 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8401 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8461 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8521 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8581 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8641 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8701 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8761 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8821 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8881 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8941 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9001 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9061 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9121 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     9181 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     9241 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     9301 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     9361 acacaggaaa cagctatgac catgattacg ccaagcgcgc ccgccgggta actcacgggg
     9421 tatccatgtc catttctgcg gcatccagcc aggatacccg tcctcgctga cgtaatatcc
     9481 cagcgccgca ccgctgtcat taatctgcac accggcacgg cagttccggc tgtcgccggt
     9541 attgttcggg ttgctgatgc gcttcgggct gaccatccgg aactgtgtcc ggaaaagccg
     9601 cgacgaactg gtatcccagg tggcctgaac gaacagttca ccgttaaagg cgtgcatggc
     9661 cacaccttcc cgaatcatca tggtaaacgt gcgttttcgc tcaacgtcaa tgcagcagca
     9721 gtcatcctcg gcaaactctt tccatgccgc ttcaacctcg cgggaaaagg cacgggcttc
     9781 ttcctccccg atgcccagat agcgccagct tgggcgatga ctgagccgga aaaaagaccc
     9841 gacgatatga tcctgatgca gctaga
//
