LOCUS       sequence-256375-        8860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Piggybac vector for CRISPR/SpCas9 applications (nuclease, base 
            editing, or epigenetic modifications) coexpressed with tdTomato-NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8860)
  TITLE     Madison Lab plasmids
REFERENCE   2  (bases 1 to 8860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BII-gR-PnTW
FEATURES             Location/Qualifiers
     source          1..8860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..39
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(308..324)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(308..324)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        580..828
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     580..600
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     751..770
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        849..924
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             2256..2309
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2316..2342
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2316..2342
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2349..3776
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             3780..3800
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3837..4425
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3890..3910)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4308..4319)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4505..4639
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4529..4548)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4583..4602
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   5417..5479
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5609..5626)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5609..5625)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5618..5640)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5767..6222
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5854..5873)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6064..6085
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6248..6352
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6353..6421,6422..7213)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6571..6590)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7384..7972
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7873..7892
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8126..8143
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8260..8281
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8296..8301,8302..8319,8320..8326)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8334..8350
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8339..8361
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8358..8374
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8358..8374
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat ccactagttc tagagggaca gccccccccc aaagccccca gggatgtaat
      361 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
      421 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
      481 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
      541 ggatacgggg aaaaggcctc cagggccgcc cccttcaccg agggcctatt tcccatgatt
      601 ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat taatttgact
      661 gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag
      721 tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag
      781 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg agacgctgac
      841 ccgtctctgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
      901 gaaaaagtgg caccgagtcg gtgctttttt gtttttgcct ccaaggcctg caggagtcaa
      961 tgggaaaaac ccattggagc caagtacact gactcaatag ggactttcca ttgggttttg
     1021 cccagtacat aaggtcaata gggggtgagt caacaggaaa gtcccattgg agccaagtac
     1081 attgagtcaa tagggacttt ccaatgggtt ttgcccagta cataaggtca atgggaggta
     1141 agccaatggg tttttcccat tactgacatg tatactgagt cattagggac tttccaatgg
     1201 gttttgccca gtacataagg tcaatagggg tgaatcaaca ggaaagtccc attggagcca
     1261 agtacactga gtcaataggg actttccatt gggttttgcc cagtacaaaa ggtcaatagg
     1321 gggtgagtca atgggttttt cccattattg gcacatacat aaggtcaata ggggtgacta
     1381 gtggagaaga gcatgcttga gggctgagtg cccctcagtg ggcagagagc acatggccca
     1441 cagtccctga gaagttgggg ggaggggtgg gcaattgaac tggtgcctag agaaggtggg
     1501 gcttgggtaa actgggaaag tgatgtggtg tactggctcc acctttttcc ccagggtggg
     1561 ggagaaccat atataagtgc agtagtctct gtgaacattc ggtttagtga acctgcaggt
     1621 cggccgccac gaccggtgcc accatgactg aatacaaacc aactgttcgc ctggcaactc
     1681 gtgatgatgt tccacgtgca gttcgcaccc tggctgctgc atttgctgac taccctgcaa
     1741 cccgtcacac tgtggaccca gaccgccaca ttgaacgtgt gactgaactg caggagctgt
     1801 tcctgacccg tgtgggcctg gacattggca aagtgtgggt ggcagatgat ggtgctgctg
     1861 tggcagtgtg gaccacccct gaatctgttg aagctggtgc agtgtttgct gagattggcc
     1921 cacgcatggc agaactgtct ggcagccgcc tggcagcaca acagcagatg gaaggtctgc
     1981 tggcaccaca ccgcccaaaa gaacctgctt ggttcctggc aactgtgggt gtgagccctg
     2041 accaccaggg taagggcctg ggctctgcag tggtgctgcc tggtgtggaa gcagctgaac
     2101 gtgcaggtgt gcctgctttc ctggagacct cagctccacg caacctgcct ttctatgaac
     2161 gcctgggctt cactgtgact gctgatgtgg aagtgccaga aggcccacgc acttggtgca
     2221 tgactcgcaa accaggtgct agccttggct ccggcgaggg caggggaagt cttctaacat
     2281 gcggggacgt ggaggaaaat cccggcccac tcggctaccc ctacgacgtg cccgactacg
     2341 ccagcggcat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc
     2401 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     2461 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     2521 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
     2581 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     2641 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
     2701 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
     2761 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
     2821 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
     2881 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
     2941 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
     3001 ccgagggccg ccaccacctg ttcctggggc atggcaccgg cagcaccggc agcggcagct
     3061 ccggcaccgc ctcctccgag gacaacaaca tggccgtcat caaagagttc atgcgcttca
     3121 aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
     3181 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
     3241 ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc
     3301 accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc
     3361 gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg
     3421 acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg
     3481 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
     3541 gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg
     3601 tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg
     3661 tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     3721 agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg tacaagggcc
     3781 ccaagaagaa gcgcaaggtg ggcgcctaag tacaagtaag agttcgcgtc tggaacaatc
     3841 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     3901 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     3961 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     4021 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     4081 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     4141 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     4201 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     4261 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     4321 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     4381 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg
     4441 gctaagcact tcgtggccgt cgatcgttta aagggaggta gtgagtcgag atatcgatac
     4501 tagtgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     4561 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     4621 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     4681 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     4741 tgatcctcta gagggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc
     4801 ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca
     4861 tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct
     4921 ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa
     4981 aagccatacc aatggagctc acggggacag ccccccccca aagcccccag ggatgtaatt
     5041 acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc cggtccggcg
     5101 ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc
     5161 acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga cacctggggg
     5221 gatacgggga aaaagaattc gataaaagtt ttgttacttt atagaagaaa ttttgagttt
     5281 ttgttttttt taataaataa ataaacataa ataaattgtt tgttgaattt attattagta
     5341 tgtaagtgta aatataataa aacttaatat ctattcaaat taataaataa acctcgatat
     5401 acagaccgat aaaacacatg cgtcaatttt acacatgatt atctttaacg tacgtcacaa
     5461 tatgattatc tttctagggt taatctagct gcgtgttctg cagcgtgtcg agcatcttca
     5521 tctgctccat cacgctgtaa aacacatttg caccgcgagt ctgcccgtcc tccacgggtt
     5581 caaaaacgtg aatgaacgag gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     5641 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     5701 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     5761 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5821 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5881 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5941 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     6001 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     6061 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     6121 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6181 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     6241 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6301 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6361 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6421 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6481 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6541 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6601 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6661 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6721 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6781 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6841 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6901 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6961 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     7021 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     7081 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     7141 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7201 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7261 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7321 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7381 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7441 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7501 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     7561 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7621 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7681 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7741 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7801 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7861 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7921 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7981 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     8041 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     8101 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     8161 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     8221 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     8281 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     8341 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcccgccg
     8401 ggtaactcac ggggtatcca tgtccatttc tgcggcatcc agccaggata cccgtcctcg
     8461 ctgacgtaat atcccagcgc cgcaccgctg tcattaatct gcacaccggc acggcagttc
     8521 cggctgtcgc cggtattgtt cgggttgctg atgcgcttcg ggctgaccat ccggaactgt
     8581 gtccggaaaa gccgcgacga actggtatcc caggtggcct gaacgaacag ttcaccgtta
     8641 aaggcgtgca tggccacacc ttcccgaatc atcatggtaa acgtgcgttt tcgctcaacg
     8701 tcaatgcagc agcagtcatc ctcggcaaac tctttccatg ccgcttcaac ctcgcgggaa
     8761 aaggcacggg cttcttcctc cccgatgccc agatagcgcc agcttgggcg atgactgagc
     8821 cggaaaaaag acccgacgat atgatcctga tgcagctaga
//
