LOCUS       sequence-256377-        8107 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Piggybac vector for bidirectional promoter reporter assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8107)
  TITLE     Madison Lab plasmids
REFERENCE   2  (bases 1 to 8107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BII-tdT-inv-GFP
FEATURES             Location/Qualifiers
     source          1..8107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..39
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     misc_feature    join(580..645,646..683)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     primer_bind     complement(703..722)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    747..795
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     polyA_signal    983..1031
                     /note="synthetic polyadenylation signal"
     misc_feature    1045..1136
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     1085..1104
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    1171..1395
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(1458..1478)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(1482..2909)
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     regulatory      2906..2915
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2971..2980
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2983..3003
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3007..3009,3010..3720)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(3007..3009,3010..3720)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3049..3070)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3310..3329)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3657..3678
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3757..3981
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4664..4726
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(4856..4873)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4856..4872)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4865..4887)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5014..5469
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5101..5120)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5311..5332
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5495..5599
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5600..5668,5669..6460)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5818..5837)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6631..7219
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7120..7139
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7373..7390
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7507..7528
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7543..7548,7549..7566,7567..7573)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7581..7597
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7586..7608
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7605..7621
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7605..7621
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat ctgtggaatg tgtgctagag ggacagcccc cccccaaagc ccccagggat
      361 gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt
      421 ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa
      481 ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc
      541 tggggggata cggggaaaag gcctggtagg ctgcagcggt agggcgcagt agtccagggt
      601 ttccttgatg atgtcatact tatcctgtcc cttttttttc cacagctcgc ggttgaggac
      661 aaactcttcg cggtctttcc agtatgcatg ggggaggcta actgaaacac ggaaggagac
      721 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacgggtg
      781 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
      841 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
      901 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
      961 catattcggc cgccacggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg
     1021 ttttttgtgt gaatcgatag tactaacata cgctctccat caaaacaaaa cgaaacaaaa
     1081 caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctctatcg
     1141 ataggtaccg catgactaca gctagacgcg ccatagagcc caccgcatcc ccagcatgcc
     1201 tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc cccagaatag
     1261 aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg
     1321 gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca gatggctggc
     1381 aactagaagg cacagtcgct cgactcacta cctcccttta aacgatcgac ggccacgaag
     1441 tgcttagctt aggcgcccac cttgcgcttc ttcttggggc ccttgtacag ctcgtccatg
     1501 ccgtacagga acaggtggtg gcggccctcg gagcgctcgt actgttccac gatggtgtag
     1561 tcctcgttgt gggaggtgat gtccagcttg gtgtccacgt agtagtagcc gggcagttgc
     1621 acgggcttct tggccatgta gatggtcttg aactccacca ggtagtggcc gccgtccttc
     1681 agcttcaggg cctggtggat ctcgcccttc agcacgccgt cgcgggggta caggcgctcg
     1741 gtggaggcct cccagcccat ggtcttcttc tgcattacgg ggccgtcggg ggggaagttg
     1801 gtgccgcgca tcttcacctt gtagatcagc gtgccgtcct gcagggagga gtcctgggtc
     1861 acggtcacca gaccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg
     1921 aaggacagct tcttgtaatc ggggatgtcg gcggggtgct tcacgtacgc cttggagccg
     1981 tacatgaact ggggggacag gatgtcccag gcgaagggca gggggccgcc cttggtcacc
     2041 ttcagcttgg cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg
     2101 aactcgtggc cgttcatgga gccctccatg cgcaccttga agcgcatgaa ctctttgatg
     2161 acggccatgt tgttgtcctc ggaggaggcg gtgccggagc tgccgctgcc ggtgctgccg
     2221 gtgccatgcc ccaggaacag gtggtggcgg ccctcggagc gctcgtactg ttccacgatg
     2281 gtgtagtcct cgttgtggga ggtgatgtcc agcttggtgt ccacgtagta gtagccgggc
     2341 agttgcacgg gcttcttggc catgtagatg gtcttgaact ccaccaggta gtggccgccg
     2401 tccttcagct tcagggcctg gtggatctcg cccttcagca cgccgtcgcg ggggtacagg
     2461 cgctcggtgg aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggggggg
     2521 aagttggtgc cgcgcatctt caccttgtag atcagcgtgc cgtcctgcag ggaggagtcc
     2581 tgggtcacgg tcaccagacc gccgtcctcg aagttcatca cgcgctccca cttgaagccc
     2641 tcggggaagg acagcttctt gtaatcgggg atgtcggcgg ggtgcttcac gtacgccttg
     2701 gagccgtaca tgaactgggg ggacaggatg tcccaggcga agggcagggg gccgcccttg
     2761 gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg
     2821 atctcgaact cgtggccgtt catggagccc tccatgcgca ccttgaagcg catgaactct
     2881 ttgatgacct cctcgccctt gctcaccatg gtggccgagt tggcaacagt accggaatgc
     2941 caagcttcct aggcggccgc gtcgaccggt gccaccatgg gccccaagaa gaagcgcaag
     3001 gtgggcgtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     3061 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     3121 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     3181 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     3241 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     3301 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     3361 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     3421 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     3481 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     3541 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     3601 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     3661 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     3721 aagctagctt aagtttaaac cgctgatcag cctcgactgt gccttctagt tgccagccat
     3781 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     3841 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     3901 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     3961 gggatgcggt gggctctatg gcctgttagg ccttctagag ggacagcccc cccccaaagc
     4021 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
     4081 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
     4141 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
     4201 tgcagacacc tggggggata cggggaaaag ccataccaat ggagctcacg gggacagccc
     4261 ccccccaaag cccccaggga tgtaattacg tccctccccc gctagggggc agcagcgagc
     4321 cgcccggggc tccgctccgg tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg
     4381 ggacagcccg ggcacgggga aggtggcacg ggatcgcttt cctctgaacg cttctcgctg
     4441 ctctttgagc ctgcagacac ctggggggat acggggaaaa agaattcgat aaaagttttg
     4501 ttactttata gaagaaattt tgagtttttg ttttttttaa taaataaata aacataaata
     4561 aattgtttgt tgaatttatt attagtatgt aagtgtaaat ataataaaac ttaatatcta
     4621 ttcaaattaa taaataaacc tcgatataca gaccgataaa acacatgcgt caattttaca
     4681 catgattatc tttaacgtac gtcacaatat gattatcttt ctagggttaa tctagctgcg
     4741 tgttctgcag cgtgtcgagc atcttcatct gctccatcac gctgtaaaac acatttgcac
     4801 cgcgagtctg cccgtcctcc acgggttcaa aaacgtgaat gaacgaggcg cgctcactgg
     4861 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     4921 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     4981 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     5041 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     5101 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     5161 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     5221 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     5281 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     5341 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     5401 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     5461 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     5521 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5581 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5641 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5701 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5761 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5821 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5881 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5941 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     6001 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     6061 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     6121 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     6181 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     6241 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6301 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6361 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6421 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6481 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6541 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6601 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6661 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6721 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     6781 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6841 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6901 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6961 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7021 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7081 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7141 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7201 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7261 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     7321 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     7381 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     7441 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     7501 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     7561 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     7621 ccatgattac gccaagcgcg cccgccgggt aactcacggg gtatccatgt ccatttctgc
     7681 ggcatccagc caggataccc gtcctcgctg acgtaatatc ccagcgccgc accgctgtca
     7741 ttaatctgca caccggcacg gcagttccgg ctgtcgccgg tattgttcgg gttgctgatg
     7801 cgcttcgggc tgaccatccg gaactgtgtc cggaaaagcc gcgacgaact ggtatcccag
     7861 gtggcctgaa cgaacagttc accgttaaag gcgtgcatgg ccacaccttc ccgaatcatc
     7921 atggtaaacg tgcgttttcg ctcaacgtca atgcagcagc agtcatcctc ggcaaactct
     7981 ttccatgccg cttcaacctc gcgggaaaag gcacgggctt cttcctcccc gatgcccaga
     8041 tagcgccagc ttgggcgatg actgagccgg aaaaaagacc cgacgatatg atcctgatgc
     8101 agctaga
//
