LOCUS       sequence-256383-       10483 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Piggybac vector for heterologous promoter reporter assay of human 
            ASCL2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10483)
  AUTHORS   Strubberg AM, Veronese Paniagua DA, Zhao T, Dublin L, Pritchard T, 
            Bayguinov PO, Fitzpatrick JAJ, Madison BB
  TITLE     The Zinc Finger Transcription Factor PLAGL2 Enhances Stem Cell Fate 
            and Activates Expression of ASCL2 in Intestinal Epithelial Cells.
  JOURNAL   Stem Cell Reports. 2018 Aug 14;11(2):410-424. doi: 
            10.1016/j.stemcr.2018.06.009. Epub 2018 Jul 12.
  PUBMED    30017821
REFERENCE   2  (bases 1 to 10483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2018.06.009"; journalName: "Stem Cell Reports"; 
            date: "2018-08-14- 14"; volume: "11"; issue: "2"; pages: "410-424"
COMMENT     Sequence Label: BII-BCA-Prom-ASCL2
FEATURES             Location/Qualifiers
     source          1..10483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..39
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     misc_feature    join(580..645,646..683)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     primer_bind     complement(703..722)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    747..795
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      969..978
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2464..2466,2467..2787)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             2794..4221
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             4225..4245
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4308..4532
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      5228..5237
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5240..5260
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5264..5266,5267..5977,5978..5983,5984..6106)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV"
     primer_bind     complement(5306..5327)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5567..5586)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5914..5935
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6143..6367
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7040..7102
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7232..7249)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7232..7248)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7241..7263)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7390..7845
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7477..7496)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7687..7708
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7871..7975
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7976..8044,8045..8836)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8194..8213)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9007..9595
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9496..9515
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9749..9766
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9883..9904
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9919..9924,9925..9942,9943..9949)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9957..9973
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9962..9984
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9981..9997
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9981..9997
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat ctgtggaatg tgtgctagag ggacagcccc cccccaaagc ccccagggat
      361 gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt
      421 ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa
      481 ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc
      541 tggggggata cggggaaaag gcctggtagg ctgcagcggt agggcgcagt agtccagggt
      601 ttccttgatg atgtcatact tatcctgtcc cttttttttc cacagctcgc ggttgaggac
      661 aaactcttcg cggtctttcc agtatgcatg ggggaggcta actgaaacac ggaaggagac
      721 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacgggtg
      781 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
      841 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
      901 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
      961 catattcggc cgccatggcc tggaagtttg aaggaattct cttcttggca tcttgggttt
     1021 ctatgggttc tgaggatttg aactcaggtc ctcacacttg cacagcaagt gctttactta
     1081 acaatctccc cagaccaact ggattgctcc aaacagcaga gaagtgacta gacttatcca
     1141 agtgctctta gcagacaccc gggaaatttc gactcactgc ccacttccca cagcaaggtt
     1201 atcaaaaggc tggattagga tgatacagcc ttgacgaatc ctggagagtc atggaacctg
     1261 caggcagccc cacactggca gtacatgctc caaggcagtg cgaaagcaga gagaagcctg
     1321 gttttggtag ccctccccca gtcggatatt tacaaagaaa gtaactccag gttactaccc
     1381 acaggataga ccacttgaac cctataggtc ttgcttggct cccaagcacc tacctagtcc
     1441 tttgagggaa aaattgggct tgctacggcc ttgagagcac acttctggga caatggggca
     1501 aaactgagac tttgacaagg tcagtgcatt tcccttcccc ttcaaccttc cctccaccgg
     1561 cctcagggga aggatgggac tgagaggtct gctggaatcc gaagaagtgt tattgaacaa
     1621 aaagtccagg aaccgaacaa aaactagtct gggtagattc accattagga aaagagaccc
     1681 agacttggag agctggctgt aaggtgcaga tgggtgacag tgtgagaaag gagtggactt
     1741 gattgtattc tctcaggtca gggcaaccgg tcctggggat tcttgaaaac acagaggcat
     1801 ggggtgtgca tgaggggcta aatgggtaca ggtgcccagg aacatgaaat ttcccattac
     1861 tggtctgaac cactcctcaa gcccttctca cactcaaggg gcacaggtgt gctcctgcaa
     1921 tgcgtggaca ccgggtcctg gtggaggagc agggtggggc ggagtgggcg tggagccttg
     1981 tgcgagacac tcccccatct ctggagccac gcgccaggcg tacgcttcct tctggccccg
     2041 gcataggacc gcgccaattg tcattggcca aacgggcgat cccagattgg ctgagacccc
     2101 ggctcccgcc tcctccgcca gggggaggga cgtgggtgcg ggtttgaggc tcgtgctacc
     2161 ctagggggtc gcgctcttct gcctcctacc tcttggtcac cgcaaagctt ggtccggttc
     2221 ttcatccggc tgcaagcgct aggtgtgcgg agacctggca gctcttgggg cttaagggct
     2281 gagcaccagg acgggtggag gtgcctgtag agtacattcg gaccctctct cggaccctct
     2341 ctcagcccct gagtgtgcgg gacctgcgga gcgcagttcg ggatctgcac tcgaggattt
     2401 ttcgaggacg caataagcta agcatctgcc cggagcatgg aagcaggccc actcggcctg
     2461 accatgggag tgcaggtgga aaccatctcc ccaggagacg ggcgcacctt ccccaagcgc
     2521 ggccagacct gtgtggtgca ctacaccggg atgcttgaag atggaaagaa agtcgattcc
     2581 tcccgggaca gaaacaagcc ctttaagttt atgctaggca agcaggaggt gatccgaggc
     2641 tgggaagaag gggttgccca gatgagtgtg ggtcagagag ccaaactgac tatatctcca
     2701 gattatgcct atggtgccac tgggcaccca ggcatcatcc caccacatgc cactctcgtc
     2761 ttcgatgtgg agcttctaaa accggaaaga tctatggtga gcaagggcga ggaggtcatc
     2821 aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag
     2881 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     2941 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc
     3001 tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc
     3061 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc
     3121 caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac
     3181 ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag
     3241 cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag
     3301 gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa
     3361 ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac
     3421 accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct ggggcatggc
     3481 accggcagca ccggcagcgg cagctccggc accgcctcct ccgaggacaa caacatggcc
     3541 gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag
     3601 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     3661 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg
     3721 tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc
     3781 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc
     3841 gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc
     3901 accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     3961 accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag
     4021 ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc
     4081 gtgcaactgc ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag
     4141 gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgtac
     4201 ggcatggacg agctgtacaa gggccccaag aagaagcgca aggtgggcgc ctaagctaag
     4261 cacttcgtgg ccgtcgatcg tttaaaggga ggtagtgagt cgagcgactg tgccttctag
     4321 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     4381 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     4441 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     4501 caggcatgct ggggatgcgg tgggctctat ggcgcgcctg caggagtcaa tgggaaaaac
     4561 ccattggagc caagtacact gactcaatag ggactttcca ttgggttttg cccagtacat
     4621 aaggtcaata gggggtgagt caacaggaaa gtcccattgg agccaagtac attgagtcaa
     4681 tagggacttt ccaatgggtt ttgcccagta cataaggtca atgggaggta agccaatggg
     4741 tttttcccat tactgacatg tatactgagt cattagggac tttccaatgg gttttgccca
     4801 gtacataagg tcaatagggg tgaatcaaca ggaaagtccc attggagcca agtacactga
     4861 gtcaataggg actttccatt gggttttgcc cagtacaaaa ggtcaatagg gggtgagtca
     4921 atgggttttt cccattattg gcacatacat aaggtcaata ggggtgacta gtggagaaga
     4981 gcatgcttga gggctgagtg cccctcagtg ggcagagagc acatggccca cagtccctga
     5041 gaagttgggg ggaggggtgg gcaattgaac tggtgcctag agaaggtggg gcttgggtaa
     5101 actgggaaag tgatgtggtg tactggctcc acctttttcc ccagggtggg ggagaaccat
     5161 atataagtgc agtagtctct gtgaacattc ggtttagtga acctgcaggt cggccgccac
     5221 gaccggtgcc accatgggcc ccaagaagaa gcgcaaggtg ggcgtgagca agggcgagga
     5281 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     5341 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     5401 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     5461 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     5521 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     5581 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     5641 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     5701 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     5761 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     5821 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     5881 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     5941 cgccgggatc actctcggca tggacgagct gtacaagaag cttagccatg gcttcccgcc
     6001 ggaggtggag gagcaggatg atggcacgct gcccatgtct tgtgcccagg agagcgggat
     6061 ggaccgtcac cctgcagcct gtgcttctgc taggatcaat gtgtgatgca tggtctcgcc
     6121 ccaaaccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     6181 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     6241 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     6301 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     6361 tctatggctt ctagagggac agcccccccc caaagccccc agggatgtaa ttacgtccct
     6421 cccccgctag ggggcagcag cgagccgccc ggggctccgc tccggtccgg cgctcccccc
     6481 gcatccccga gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc
     6541 gctttcctct gaacgcttct cgctgctctt tgagcctgca gacacctggg gggatacggg
     6601 gaaaagccat accaatggag ctcacgggga cagccccccc ccaaagcccc cagggatgta
     6661 attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg ctccggtccg
     6721 gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca cggggaaggt
     6781 ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc agacacctgg
     6841 ggggatacgg ggaaaaagaa ttcgataaaa gttttgttac tttatagaag aaattttgag
     6901 tttttgtttt ttttaataaa taaataaaca taaataaatt gtttgttgaa tttattatta
     6961 gtatgtaagt gtaaatataa taaaacttaa tatctattca aattaataaa taaacctcga
     7021 tatacagacc gataaaacac atgcgtcaat tttacacatg attatcttta acgtacgtca
     7081 caatatgatt atctttctag ggttaatcta gctgcgtgtt ctgcagcgtg tcgagcatct
     7141 tcatctgctc catcacgctg taaaacacat ttgcaccgcg agtctgcccg tcctccacgg
     7201 gttcaaaaac gtgaatgaac gaggcgcgct cactggccgt cgttttacaa cgtcgtgact
     7261 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     7321 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     7381 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     7441 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     7501 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     7561 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     7621 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     7681 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     7741 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     7801 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     7861 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7921 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     7981 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     8041 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     8101 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     8161 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     8221 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     8281 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     8341 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     8401 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     8461 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     8521 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     8581 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     8641 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     8701 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     8761 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     8821 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     8881 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8941 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     9001 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     9061 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     9121 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     9181 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     9241 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     9301 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     9361 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     9421 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     9481 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     9541 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     9601 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     9661 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     9721 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     9781 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     9841 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     9901 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     9961 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcccg
    10021 ccgggtaact cacggggtat ccatgtccat ttctgcggca tccagccagg atacccgtcc
    10081 tcgctgacgt aatatcccag cgccgcaccg ctgtcattaa tctgcacacc ggcacggcag
    10141 ttccggctgt cgccggtatt gttcgggttg ctgatgcgct tcgggctgac catccggaac
    10201 tgtgtccgga aaagccgcga cgaactggta tcccaggtgg cctgaacgaa cagttcaccg
    10261 ttaaaggcgt gcatggccac accttcccga atcatcatgg taaacgtgcg ttttcgctca
    10321 acgtcaatgc agcagcagtc atcctcggca aactctttcc atgccgcttc aacctcgcgg
    10381 gaaaaggcac gggcttcttc ctccccgatg cccagatagc gccagcttgg gcgatgactg
    10441 agccggaaaa aagacccgac gatatgatcc tgatgcagct aga
//
