LOCUS       sequence-256389-        9048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Piggybac vector for SRIRACCHA-mediated mutation enrichment 
            (surrogate target for HDR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Wen Y, Liao G, Pritchard T, Zhao TT, Connelly JP, Pruett-Miller SM, 
            Blanc V, Davidson NO, Madison BB
  TITLE     A stable but reversible integrated surrogate reporter for assaying 
            CRISPR/Cas9-stimulated homology-directed repair.
  JOURNAL   J Biol Chem. 2017 Apr 14;292(15):6148-6162. doi: 
            10.1074/jbc.M117.777722. Epub 2017 Feb 22.
  PUBMED    28228480
REFERENCE   2  (bases 1 to 9048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M117.777722"; journalName: "J Biol Chem"; date: 
            "2017-04-14- 14"; volume: "292"; issue: "15"; pages: "6148-6162"
COMMENT     Sequence Label: BII-C3HTK
FEATURES             Location/Qualifiers
     source          1..9048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..39
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     CDS             1931..2950
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             1931..2950
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    2981..3546
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2997..3548
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3141..3158)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3368..3387
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3548..4678
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMIQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    4693..4827
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4717..4736)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4771..4790
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   5605..5667
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5797..5814)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5797..5813)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5806..5828)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5955..6410
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6042..6061)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6252..6273
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6436..6540
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6541..6609,6610..7401)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6759..6778)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7572..8160
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8061..8080
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8314..8331
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8448..8469
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8484..8489,8490..8507,8508..8514)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8522..8538
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8527..8549
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8546..8562
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8546..8562
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat ctgtggaatg tgtgctagag ggacagcccc cccccaaagc ccccagggat
      361 gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt
      421 ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa
      481 ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc
      541 tggggggata cggggaaaag gcctccaagg cctgcaggag tcaatgggaa aaacccattg
      601 gagccaagta cactgactca atagggactt tccattgggt tttgcccagt acataaggtc
      661 aatagggggt gagtcaacag gaaagtccca ttggagccaa gtacattgag tcaataggga
      721 ctttccaatg ggttttgccc agtacataag gtcaatggga ggtaagccaa tgggtttttc
      781 ccattactga catgtatact gagtcattag ggactttcca atgggttttg cccagtacat
      841 aaggtcaata ggggtgaatc aacaggaaag tcccattgga gccaagtaca ctgagtcaat
      901 agggactttc cattgggttt tgcccagtac aaaaggtcaa tagggggtga gtcaatgggt
      961 ttttcccatt attggcacat acataaggtc aataggggtg actagtggag aagagcatgc
     1021 ttgagggctg agtgcccctc agtgggcaga gagcacatgg cccacagtcc ctgagaagtt
     1081 ggggggaggg gtgggcaatt gaactggtgc ctagagaagg tggggcttgg gtaaactggg
     1141 aaagtgatgt ggtgtactgg ctccaccttt ttccccaggg tgggggagaa ccatatataa
     1201 gtgcagtagt ctctgtgaac attcggttta gtgaacctgc aggtcggccg ccacgaccgg
     1261 tgccaccatg actgaataca aaccaactgt tcgcctggca actcgtgatg atgttccacg
     1321 tgcagttcgc accctggctg ctgcatttgc tgactaccct gcaacccgtc acactgtgga
     1381 cccagaccgc cacattgaac gtgtgactga actgcaggag ctgttcctga cccgtgtggg
     1441 cctggacatt ggcaaagtgt gggtggcaga tgatggtgct gctgtggcag tgtggaccac
     1501 ccctgaatct gttgaagctg gtgcagtgtt tgctgagatt ggcccacgca tggcagaact
     1561 gtctggcagc cgcctggcag cacaacagca gatggaaggt ctgctggcac cacaccgccc
     1621 aaaagaacct gcttggttcc tggcaactgt gggtgtgagc cctgaccacc agggtaaggg
     1681 cctgggctct gcagtggtgc tgcctggtgt ggaagcagct gaacgtgcag gtgtgcctgc
     1741 tttcctggag acctcagctc cacgcaacct gcctttctat gaacgcctgg gcttcactgt
     1801 gactgctgat gtggaagtgc cagaaggccc acgcacttgg tgcatgactc gcaaaccagg
     1861 tgctaggtga ccgcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca
     1921 ctcggcaata aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     1981 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     2041 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     2101 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     2161 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     2221 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     2281 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     2341 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     2401 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     2461 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     2521 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     2581 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     2641 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     2701 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     2761 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     2821 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     2881 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     2941 aaaggaatag agcggccgct cgaggtgcac gaattgcaat tccgcccccc cccctaacgt
     3001 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     3061 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     3121 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     3181 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     3241 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     3301 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     3361 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     3421 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     3481 gttaaaaaag ctctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     3541 tgataatatg gcttcgtacc ccggccatca gcacgcgtct gcgttcgacc aggctgcgcg
     3601 ttctcgcggc catagcaacc gacgtacggc gttgcgccct cgccggcagc aagaagccac
     3661 ggaagtccgc ccggagcaga aaatgcccac gctactgcgg gtttatatag acggtcccca
     3721 cgggatgggg aaaaccacca ccacgcaact gctggtggcc ctgggttcgc gcgacgatat
     3781 cgtctacgta cccgagccga tgacttactg gcgggtgctg ggggcttccg agacaatcgc
     3841 gaacatctac accacacaac accgccttga ccagggtgag atatcggccg gggacgcggc
     3901 ggtggtaatg acaagcgccc agataacaat gggcatgcct tatgccgtga ccgacgccgt
     3961 tctggctcct catatcgggg gggaggctgg gagctcacat gccccgcccc cggccctcac
     4021 cctcatcttc gaccgccatc ccatcgccgc cctcctgtgc tacccggccg cgcgatacct
     4081 tatgggcagc atgacccccc aggccgtgct ggcgttcgtg gccctcatcc cgccgacctt
     4141 gcccggcaca aacatcgtgt tgggggccct tccggaggac agacacatcg accgcctggc
     4201 caaacgccag cgccccggcg agcggcttga cctggctatg ctggccgcga ttcgccgcgt
     4261 ttacgggctg cttgccaata cggtgcggta tctgcagggc ggcgggtcgt ggcgggagga
     4321 ttggggacag ctttcgggga cggccgtgcc gccccagggt gccgagcccc agagcaacgc
     4381 gggcccacga ccccatatcg gggacacgtt atttaccctg tttcgggccc ccgagttgct
     4441 ggcccccaac ggcgacctgt acaacgtgtt tgcctgggcc ttggacgtct tggccaaacg
     4501 cctccgtccc atgcacgtct ttatcctgga ttacgaccaa tcgcccgccg gctgccggga
     4561 cgccctgctg caacttacct ccgggatgat ccagacccac gtcaccaccc caggctccat
     4621 accgacgatc tgcgacctgg cgcgcacgtt tgcccgggag atgggggagg ctaactgaaa
     4681 cacggaacta gtgatccaga catgataaga tacattgatg agtttggaca aaccacaact
     4741 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     4801 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     4861 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     4921 gctgattatg atcctctaga gggacagccc ccccccaaag cccccaggga tgtaattacg
     4981 tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg tccggcgctc
     5041 cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg
     5101 ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac ctggggggat
     5161 acggggaaaa gccataccaa tggagctcac ggggacagcc cccccccaaa gcccccaggg
     5221 atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg
     5281 gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg
     5341 aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca
     5401 cctgggggga tacggggaaa aagaattcga taaaagtttt gttactttat agaagaaatt
     5461 ttgagttttt gtttttttta ataaataaat aaacataaat aaattgtttg ttgaatttat
     5521 tattagtatg taagtgtaaa tataataaaa cttaatatct attcaaatta ataaataaac
     5581 ctcgatatac agaccgataa aacacatgcg tcaattttac acatgattat ctttaacgta
     5641 cgtcacaata tgattatctt tctagggtta atctagctgc gtgttctgca gcgtgtcgag
     5701 catcttcatc tgctccatca cgctgtaaaa cacatttgca ccgcgagtct gcccgtcctc
     5761 cacgggttca aaaacgtgaa tgaacgaggc gcgctcactg gccgtcgttt tacaacgtcg
     5821 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     5881 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     5941 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     6001 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     6061 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     6121 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     6181 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     6241 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     6301 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     6361 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     6421 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     6481 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     6541 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     6601 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     6661 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     6721 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     6781 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     6841 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     6901 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     6961 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     7021 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     7081 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     7141 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     7201 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     7261 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     7321 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     7381 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     7441 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     7501 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7561 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7621 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7681 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     7741 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7801 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7861 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7921 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7981 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     8041 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     8101 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     8161 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     8221 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     8281 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     8341 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     8401 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     8461 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     8521 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     8581 gcccgccggg taactcacgg ggtatccatg tccatttctg cggcatccag ccaggatacc
     8641 cgtcctcgct gacgtaatat cccagcgccg caccgctgtc attaatctgc acaccggcac
     8701 ggcagttccg gctgtcgccg gtattgttcg ggttgctgat gcgcttcggg ctgaccatcc
     8761 ggaactgtgt ccggaaaagc cgcgacgaac tggtatccca ggtggcctga acgaacagtt
     8821 caccgttaaa ggcgtgcatg gccacacctt cccgaatcat catggtaaac gtgcgttttc
     8881 gctcaacgtc aatgcagcag cagtcatcct cggcaaactc tttccatgcc gcttcaacct
     8941 cgcgggaaaa ggcacgggct tcttcctccc cgatgcccag atagcgccag cttgggcgat
     9001 gactgagccg gaaaaaagac ccgacgatat gatcctgatg cagctaga
//
