LOCUS       sequence-256390-        8850 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Piggybac vector for SRIRACCHA-mediated mutation enrichment 
            (surrogate target for HDR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Wen Y, Liao G, Pritchard T, Zhao TT, Connelly JP, Pruett-Miller SM, 
            Blanc V, Davidson NO, Madison BB
  TITLE     A stable but reversible integrated surrogate reporter for assaying 
            CRISPR/Cas9-stimulated homology-directed repair.
  JOURNAL   J Biol Chem. 2017 Apr 14;292(15):6148-6162. doi: 
            10.1074/jbc.M117.777722. Epub 2017 Feb 22.
  PUBMED    28228480
REFERENCE   2  (bases 1 to 8850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M117.777722"; journalName: "J Biol Chem"; date: 
            "2017-04-14- 14"; volume: "292"; issue: "15"; pages: "6148-6162"
COMMENT     Sequence Label: BII-B3HTK
FEATURES             Location/Qualifiers
     source          1..8850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..39
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      1262..1271
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1268..1663
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             1733..2752
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             1733..2752
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    2783..3348
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2799..3350
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2943..2960)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3170..3189
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3350..4480
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMIQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    4495..4629
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4519..4538)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4573..4592
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   5407..5469
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5599..5616)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5599..5615)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5608..5630)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5757..6212
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5844..5863)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6054..6075
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6238..6342
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6343..6411,6412..7203)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6561..6580)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7374..7962
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7863..7882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8116..8133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8250..8271
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8286..8291,8292..8309,8310..8316)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8324..8340
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8329..8351
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8348..8364
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8348..8364
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat ctgtggaatg tgtgctagag ggacagcccc cccccaaagc ccccagggat
      361 gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt
      421 ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa
      481 ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc
      541 tggggggata cggggaaaag gcctccaagg cctgcaggag tcaatgggaa aaacccattg
      601 gagccaagta cactgactca atagggactt tccattgggt tttgcccagt acataaggtc
      661 aatagggggt gagtcaacag gaaagtccca ttggagccaa gtacattgag tcaataggga
      721 ctttccaatg ggttttgccc agtacataag gtcaatggga ggtaagccaa tgggtttttc
      781 ccattactga catgtatact gagtcattag ggactttcca atgggttttg cccagtacat
      841 aaggtcaata ggggtgaatc aacaggaaag tcccattgga gccaagtaca ctgagtcaat
      901 agggactttc cattgggttt tgcccagtac aaaaggtcaa tagggggtga gtcaatgggt
      961 ttttcccatt attggcacat acataaggtc aataggggtg actagtggag aagagcatgc
     1021 ttgagggctg agtgcccctc agtgggcaga gagcacatgg cccacagtcc ctgagaagtt
     1081 ggggggaggg gtgggcaatt gaactggtgc ctagagaagg tggggcttgg gtaaactggg
     1141 aaagtgatgt ggtgtactgg ctccaccttt ttccccaggg tgggggagaa ccatatataa
     1201 gtgcagtagt ctctgtgaac attcggttta gtgaacctgc aggtcggccg ccacgaccgg
     1261 tgccaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc
     1321 tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag
     1381 cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga
     1441 actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc
     1501 gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct
     1561 cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt
     1621 tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggcgctaggt gaccgcaata
     1681 aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cactcggcaa taaagcctga
     1741 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     1801 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     1861 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     1921 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
     1981 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     2041 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
     2101 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     2161 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     2221 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     2281 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     2341 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     2401 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     2461 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     2521 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     2581 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     2641 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     2701 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agagcggccg
     2761 ctcgaggtgc acgaattgca attccgcccc cccccctaac gttactggcc gaagccgctt
     2821 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     2881 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     2941 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     3001 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     3061 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     3121 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     3181 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     3241 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa agctctaggc
     3301 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggcttcgta
     3361 ccccggccat cagcacgcgt ctgcgttcga ccaggctgcg cgttctcgcg gccatagcaa
     3421 ccgacgtacg gcgttgcgcc ctcgccggca gcaagaagcc acggaagtcc gcccggagca
     3481 gaaaatgccc acgctactgc gggtttatat agacggtccc cacgggatgg ggaaaaccac
     3541 caccacgcaa ctgctggtgg ccctgggttc gcgcgacgat atcgtctacg tacccgagcc
     3601 gatgacttac tggcgggtgc tgggggcttc cgagacaatc gcgaacatct acaccacaca
     3661 acaccgcctt gaccagggtg agatatcggc cggggacgcg gcggtggtaa tgacaagcgc
     3721 ccagataaca atgggcatgc cttatgccgt gaccgacgcc gttctggctc ctcatatcgg
     3781 gggggaggct gggagctcac atgccccgcc cccggccctc accctcatct tcgaccgcca
     3841 tcccatcgcc gccctcctgt gctacccggc cgcgcgatac cttatgggca gcatgacccc
     3901 ccaggccgtg ctggcgttcg tggccctcat cccgccgacc ttgcccggca caaacatcgt
     3961 gttgggggcc cttccggagg acagacacat cgaccgcctg gccaaacgcc agcgccccgg
     4021 cgagcggctt gacctggcta tgctggccgc gattcgccgc gtttacgggc tgcttgccaa
     4081 tacggtgcgg tatctgcagg gcggcgggtc gtggcgggag gattggggac agctttcggg
     4141 gacggccgtg ccgccccagg gtgccgagcc ccagagcaac gcgggcccac gaccccatat
     4201 cggggacacg ttatttaccc tgtttcgggc ccccgagttg ctggccccca acggcgacct
     4261 gtacaacgtg tttgcctggg ccttggacgt cttggccaaa cgcctccgtc ccatgcacgt
     4321 ctttatcctg gattacgacc aatcgcccgc cggctgccgg gacgccctgc tgcaacttac
     4381 ctccgggatg atccagaccc acgtcaccac cccaggctcc ataccgacga tctgcgacct
     4441 ggcgcgcacg tttgcccggg agatggggga ggctaactga aacacggaac tagtgatcca
     4501 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     4561 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     4621 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     4681 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcctcta
     4741 gagggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg
     4801 gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca tccccgagcc
     4861 ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct ttcctctgaa
     4921 cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa aagccatacc
     4981 aatggagctc acggggacag ccccccccca aagcccccag ggatgtaatt acgtccctcc
     5041 cccgctaggg ggcagcagcg agccgcccgg ggctccgctc cggtccggcg ctccccccgc
     5101 atccccgagc cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc acgggatcgc
     5161 tttcctctga acgcttctcg ctgctctttg agcctgcaga cacctggggg gatacgggga
     5221 aaaagaattc gataaaagtt ttgttacttt atagaagaaa ttttgagttt ttgttttttt
     5281 taataaataa ataaacataa ataaattgtt tgttgaattt attattagta tgtaagtgta
     5341 aatataataa aacttaatat ctattcaaat taataaataa acctcgatat acagaccgat
     5401 aaaacacatg cgtcaatttt acacatgatt atctttaacg tacgtcacaa tatgattatc
     5461 tttctagggt taatctagct gcgtgttctg cagcgtgtcg agcatcttca tctgctccat
     5521 cacgctgtaa aacacatttg caccgcgagt ctgcccgtcc tccacgggtt caaaaacgtg
     5581 aatgaacgag gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     5641 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     5701 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     5761 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     5821 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     5881 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     5941 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     6001 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     6061 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     6121 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     6181 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     6241 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     6301 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     6361 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     6421 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     6481 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     6541 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     6601 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     6661 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6721 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     6781 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     6841 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     6901 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6961 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     7021 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     7081 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     7141 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     7201 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     7261 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     7321 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     7381 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7441 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7501 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     7561 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7621 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7681 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7741 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7801 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7861 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7921 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7981 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     8041 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     8101 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     8161 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     8221 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     8281 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     8341 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcccgccg ggtaactcac
     8401 ggggtatcca tgtccatttc tgcggcatcc agccaggata cccgtcctcg ctgacgtaat
     8461 atcccagcgc cgcaccgctg tcattaatct gcacaccggc acggcagttc cggctgtcgc
     8521 cggtattgtt cgggttgctg atgcgcttcg ggctgaccat ccggaactgt gtccggaaaa
     8581 gccgcgacga actggtatcc caggtggcct gaacgaacag ttcaccgtta aaggcgtgca
     8641 tggccacacc ttcccgaatc atcatggtaa acgtgcgttt tcgctcaacg tcaatgcagc
     8701 agcagtcatc ctcggcaaac tctttccatg ccgcttcaac ctcgcgggaa aaggcacggg
     8761 cttcttcctc cccgatgccc agatagcgcc agcttgggcg atgactgagc cggaaaaaag
     8821 acccgacgat atgatcctga tgcagctaga
//
