LOCUS       sequence-256404-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mutant yeast STE2 coding sequence in vector for expression in yeast 
            S. cerevisiae, with Gal1 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  TITLE     Wagner Lab plasmids
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pYES2-STE2-1D4
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..22
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3..21
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgtaatacga ctcactatag ggaatattaa gcttggtacc gagctcggat ccactagtaa
       61 cggccgccag tgtgctggaa ttcataatgt ctgatgcggc tccttcattg agcaatctat
      121 tttatgatcc aacgtataat cctggtcaaa gcaccattaa ctacacttcc atatatggga
      181 atggatctac catcactttc gatgagttgc aaggtttagt taacagtact gttactcagg
      241 ccattatgtt tggtgtcaga tgtggtgcag ctgctttgac tttgattgtc atgtggatga
      301 catcgagaag cagaaaaacg ccgattttca ttatcaacca agtttcattg tttttaatca
      361 ttttgcattc tgcactctat tttaaatatt tactgtctaa ttactcttca gtgacttacg
      421 ctctcaccgg atttcctcag ttcatcagta gaggtgacgt tcatgtttat ggtgctacaa
      481 atataattca agtccttctt gtggcttcta ttgagacttc actggtgttt cagataaaag
      541 ttattttcac aggcgacaac ttcaaaagga taggtttgat gctgacgtcg atatctttca
      601 ctttagggat tgctacagtt accatgtatt ttgtaagcgc tgttaaaggt atgattgtga
      661 cttataatga tgttagtgcc acccaagata aatacttcaa tgcatccaca attttacttg
      721 catcctcaat aaactttatg tcatttgtcc tggtagttaa attgatttta gctattagat
      781 caagaagatt ccttggtctc aagcagttcg atagtttcca tattttactc ataatgtcat
      841 gtcaatcttt gttggttcca tcgataatat tcatcctcgc atacagtttg aaaccaaacc
      901 agggaacaga tgtcttgact actgttgcaa cattacttgc tgtattgtct ttaccattat
      961 catcaatgtg ggccacggct gctaataatg cgtcgtcgac caccgtctcc aagacagaga
     1021 ccagccaagt ggcgcctgcc
//
