LOCUS       sequence-256407-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human ARC105 coding sequence in vector for in vitro transcription 
            and protein expression, with T7 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Yang F, Vought BW, Satterlee JS, Walker AK, Jim Sun ZY, Watts JL, 
            DeBeaumont R, Saito RM, Hyberts SG, Yang S, Macol C, Iyer L, Tjian 
            R, van den Heuvel S, Hart AC, Wagner G, Naar AM
  TITLE     An ARC/Mediator subunit required for SREBP control of cholesterol 
            and lipid homeostasis.
  JOURNAL   Nature. 2006 Aug 10;442(7103):700-4. doi: 10.1038/nature04942. Epub 
            2006 Jun 21.
  PUBMED    16799563
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature04942"; journalName: "Nature"; date: "2006-08-10- 
            10"; volume: "442"; issue: "7103"; pages: "700-4"
COMMENT     Sequence Label: pETM30-ARC105
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..21
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             244..897
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     855..877
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gaaaacctgt acttccaggg tgggcaagag accgactggc ggagcaccgc cttccggcag
       61 aagctggtca gtcaaatcga ggatgccatg aggaaagctg gtgtggcaca cagtaaatcc
      121 agcaaggata tggagagcca tgttttcctg aaggccaaga cccgggacga atacctttct
      181 ctcgtggcca ggctcattat ccattttcga gacattcata acaagaaatc tcaagcttcc
      241 gtcatgtccc ctatactagg ttattggaaa attaagggcc ttgtgcaacc cactcgactt
      301 cttttggaat atcttgaaga aaaatatgaa gagcatttgt atgagcgcga tgaaggtgat
      361 aaatggcgaa acaaaaagtt tgaattgggt ttggagtttc ccaatcttcc ttattatatt
      421 gatggtgatg ttaaattaac acagtctatg gccatcatac gttatatagc tgacaagcac
      481 aacatgttgg gtggttgtcc aaaagagcgt gcagagattt caatgcttga aggagcggtt
      541 ttggatatta gatacggtgt ttcgagaatt gcatatagta aagactttga aactctcaaa
      601 gttgattttc ttagcaagct acctgaaatg ctgaaaatgt tcgaagatcg tttatgtcat
      661 aaaacatatt taaatggtga tcatgtaacc catcctgact tcatgttgta tgacgctctt
      721 gatgttgttt tatacatgga cccaatgtgc ctggatgcgt tcccaaaatt agtttgtttt
      781 aaaaaacgta ttgaagctat cccacaaatt gataagtact tgaaatccag caagtatata
      841 gcatggcctt tgcagggctg gcaagccacg tttggtggtg gcgaccatcc tccaaaatcg
      901 gatctggttc c
//
