LOCUS       sequence-256408-         360 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human BP1 fragment of SREBF1 with GB1 solubility tag in vector for 
            in vitro expression, with T7 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 360)
  TITLE     Wagner Lab plasmids
REFERENCE   2  (bases 1 to 360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHIS-GB1-BP1
FEATURES             Location/Qualifiers
     source          1..360
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..243
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             256..276
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac gattacgata tcccaacgac cgaaaacctg
       61 tattttctgg gcgccatgga gtacaagctt atcctgaacg gtaaaaccct gaaaggtgaa
      121 accaccaccg aagctgttga cgctgctacc gcggaaaaag ttttcaaaca gtacgctaac
      181 gacaacggtg ttgacggtga atggacctac gacgacgcta ccaaaacctt cacggtaacc
      241 gaaatcccaa cgaccgaaaa cctgtatttt cagggcgaat tcgaaccgtg cgatctggat
      301 gcggcgctgc tgaccgacat tgaagatatg ctgcaactga ttaacaatca ggatagcgac
//
