LOCUS       sequence-256412-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast STE2 coding sequence in vector for in vitro transcription and 
            protein synthesis, with T7 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  TITLE     Wagner Lab plasmids
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET-24a-STE2
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..21
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             940..960
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             961..984
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gaaaacctgt acttccaggg tatgtctgat gctgctcctt ctctttccaa ccttttttat
       61 gaccctacgt acaatccagg tcagtctact atcaactaca cttccatcta tggaaatggt
      121 tccactatta cctttgatga acttcaaggt cttgttaaca gtactgttac tcaagccatt
      181 atgttcggag tccgttgtgg tgctgcagcc cttactctta ttgttatgtg gatgacctct
      241 agatctagaa agactccaat attcattatc aaccaggtct ccttgttcct tatcattttg
      301 cactctgcct tgtatttcaa gtatcttctt tctaactact cctctgttac atatgccttg
      361 actggtttcc ctcaatttat ttctagaggt gacgttcacg tgtacggtgc cactaacatc
      421 attcaggttt tgcttgttgc ttctattgag acctcactag tttttcaaat taaggtcatt
      481 ttcaccggtg acaattttaa gagaattgga cttatgttga cttccatctc ctttacccta
      541 ggtatcgcta ctgtcactat gtattttgtt tccgccgtta agggtatgat cgtcacttac
      601 aatgacgtct ccgctaccca ggacaaatac tttaacgctt ccactatttt gctagcctct
      661 tccattaatt tcatgtcttt tgttttggtt gtcaaattga ttcttgccat tcgttctcgt
      721 cgttttcttg gtttgaaaca atttgactcc tttcacattc ttcttatcat gtcctgtcaa
      781 tctcttcttg ttccatctat catctttatc ttggcctatt ctcttaagcc aaatcaaggt
      841 accgacgttt tgactactgt cgccactctt ttggctgtcc tttctcttcc tttgtcttct
      901 atgtgggcta ccgctgccaa taatgcctcc ttgggcccag aaaacctgta cttccagggt
      961 caccatcatc accaccatca tcactaataa
//
