LOCUS       sequence-256414-        1134 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human CXCR4 coding sequence in vector for in vitro transcription and
            protein synthesis, with T7 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1134)
  TITLE     Wagner Lab plasmids
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET-24a-CXCR4
FEATURES             Location/Qualifiers
     source          1..1134
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..21
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1087..1107
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1108..1131
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gaaaacctgt acttccaggg tatggaagga atctctattt atacctctga taactatact
       61 gaagaaatgg gatccggaga ttacgattcc atgaaagaac cttgtttccg tgaggaaaac
      121 gccaacttca acaaaatatt ccttccaact atctattcca tcattttttt gaccggaatc
      181 gttggtaatg gactagttat tttggttatg ggataccaaa aaaaacttcg ttccatgact
      241 gataagtaca gacttcatct ttctgttgct gatttgcttt tcgttattac ccttcctttc
      301 tgggccgtcg acgctgtcgc caattggtac tttggaaatt tcctttgtaa ggccgttcac
      361 gttatttata ccgtcaacct ttactcctca gtactgattc ttgcttttat ttcccttgat
      421 agataccttg ccatcgtgca tgccactaac tctcagagac ctagaaagct tcttgccgag
      481 aaagtcgtct acgtaggagt ttggattcca gctcttttgt tgaccattcc agatttcatt
      541 tttgctaacg tctccgaggc tgatgatcgt tacatttgtg accgttttta cccaaatgac
      601 ctttgggttg tcgttttcca atttcaacat atcatggtcg gacttatttt gccaggaatt
      661 gtcattcttt cctgttactg catcatcatc tccaagcttt ctcattctaa aggtcaccag
      721 aaaagaaaag ccttgaagac caccgtcatc ttgattcttg ccttttttgc ttgttggctt
      781 ccatattata tcggtatttc catcgattcc ttcatccttc ttgaaattat taaacaagga
      841 tgtgaatttg agaacactgt ccataagtgg atctccatta ccgaggcctt ggctttcttc
      901 cattgctgtc ttaaccctat tttgtatgcc tttttgggcg ccaaattcaa gacttccgct
      961 caacacgcct tgacctctgt ttctcgtgga tcttctctta agattttgtc taaaggaaaa
     1021 cgtggaggac actcctctgt ctccaccgaa tctgaatcct cttcctttca ttcctctgga
     1081 gggcccgaaa acctgtactt ccagggtcac catcatcacc accatcatca ctaa
//
