LOCUS       sequence-256430-         611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Covalently circularized nanodisc NW9 for studying membrane proteins 
            and viral entry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Nasr ML, Baptista D, Strauss M, Sun ZJ, Grigoriu S, Huser S, 
            Pluckthun A, Hagn F, Walz T, Hogle JM, Wagner G
  TITLE     Covalently circularized nanodiscs for studying membrane proteins and
            viral entry.
  JOURNAL   Nat Methods. 2017 Jan;14(1):49-52. doi: 10.1038/nmeth.4079. Epub 
            2016 Nov 21.
  PUBMED    27869813
REFERENCE   2  (bases 1 to 611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4079"; journalName: "Nat Methods"; date: "2017-01"; 
            volume: "14"; issue: "1"; pages: "49-52"
COMMENT     Sequence Label: NW9
FEATURES             Location/Qualifiers
     source          1..611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..32
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             33..53
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             585..602
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ccatgggttc aagccaccac caccaccacc acgaaaatct gtactttcaa ggcagcacgt
       61 tcagcaaact gcgcgaacaa ctgggtccgg ttacccagga attttgggat aacctggaaa
      121 aagaaacgga aggcctgcgc caggaaatga gcaaagatct ggaagaagtg aaagccaaag
      181 ttcaaccgta tctggatgac ttccagaaaa aatggcaaga agaaatggaa ctgtaccgcc
      241 agaaagttga accgctgggt gaagaaatgc gtgatcgcgc acgtgctcat gttgatgccc
      301 tgcgtaccca cctggcaccg tattcggatg aactgcgtca gcgtctggcc gcacgtctgg
      361 aagcactgaa agaaaatggc ggtgcacgtc tggctgaata ccatgcgaaa gccaccgaac
      421 acctgagcac gctgtctgaa aaagcgaaac cggccctgga agacctgcgt cagggtctgc
      481 tgccggtcct ggaaagtttt aaagttagtt tcctgtccgc gctggaagaa tatacgaaaa
      541 aactgaacac gcaactgccg ggtacgggcg ccgctgcact ggaacatcat catcatcatc
      601 actaactcga g
//
