LOCUS       sequence-256432-        1758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Covalently circularized nanodisc NW30 for studying membrane proteins
            and viral entry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Nasr ML, Baptista D, Strauss M, Sun ZJ, Grigoriu S, Huser S, 
            Pluckthun A, Hagn F, Walz T, Hogle JM, Wagner G
  TITLE     Covalently circularized nanodiscs for studying membrane proteins and
            viral entry.
  JOURNAL   Nat Methods. 2017 Jan;14(1):49-52. doi: 10.1038/nmeth.4079. Epub 
            2016 Nov 21.
  PUBMED    27869813
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4079"; journalName: "Nat Methods"; date: "2017-01"; 
            volume: "14"; issue: "1"; pages: "49-52"
COMMENT     Sequence Label: NW30
FEATURES             Location/Qualifiers
     source          1..1758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..33
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..54
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1732..1749
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 catatgggca gctctcatca ccatcaccat cacgaaaacc tgtattttca gggcagtacc
       61 ttctccaaac tgcgcgaaca gctgggtccg gtgacgcaag aattctggga taacctggaa
      121 aaagaaaccg aaggcctgcg tcaggaaatg agtaaagacc tggaagaagt gaaagccaaa
      181 gttcaaccgt atctggatga cttccagaaa aaatggcaag aagaaatgga actgtaccgc
      241 cagaaagtgg aaccgctgcg tgccgaactg caggaaggcg cacgccaaaa actgcatgaa
      301 ctgcaagaaa aactgtcacc gctgggtgaa gaaatgcgtg atcgcgcccg tgcacatgtt
      361 gacgctctgc gtacccacct ggcgccgtat tcggatgaac tgcgtcagcg tctggccgca
      421 cgtctggaag cgctgaaaga aaacggcggt gcccgtctgg cagaatacca tgctaaagcg
      481 accgaacacc tgtcaacgct gtcggaaaaa gctaaaccgg ccctggaaga tctgcgccag
      541 ggtctgctgc cggtgctgga atcatttaaa gttagcttcc tgtctgcgct ggaagaatat
      601 acgaaaaaac tgaacaccca gggcacgccg gttacccagg aattttggga caatctggaa
      661 aaagaaacgg aaggcctgcg ccaggaaatg tcgaaagacc tggaagaagt caaagccaaa
      721 gtgcagccgt acctggacga ttttcagaaa aaatggcaag aagaaatgga gctgtaccgc
      781 cagaaagtcg aaccgctgcg cgcggaactg caggaaggcg cccgtcagaa actgcatgaa
      841 ctgcaggaaa aactgagccc gctgggcgaa gaaatgcgtg atcgtgcccg cgcacatgtt
      901 gatgccctgc gcacccacct ggcaccgtat tctgatgaac tgcgccagcg cctggcagct
      961 cgtctggaag ccctgaaaga aaatggcggt gcccgcctgg ccgaatatca cgccaaagcc
     1021 accgaacacc tgagtacgct gtccgaaaaa gctaaaccgg cgctggaaga tctgcgccaa
     1081 ggtctgctgc cggtcctgga atcatttaaa gtgtcattcc tgtcggcact ggaagaatac
     1141 acgaaaaaac tgaacacgca gggcaccccg gttacccagg aattctggga caacctggaa
     1201 aaggagacgg aaggcctgcg tcaagaaatg agcaaagatc tggaagaagt taaagcgaaa
     1261 gtccagccgt acctggatga ctttcagaaa aaatggcaag aagaaatgga gttataccgc
     1321 cagaaagttg aaccgctgcg tgcagaactg caggaaggcg ctcgtcagaa actgcacgag
     1381 ttacaagaaa aactgagtcc gctgggagag gaaatgcgcg accgtgcccg tgcacatgtt
     1441 gatgcactgc gtacgcatct ggcaccgtat tccgacgaac tgcgtcaacg cctggccgcc
     1501 cgcctggaag cgttaaagga aaatggcggt gcccgtctgg ccgaatatca cgcgaaagcc
     1561 acggaacatc tgagcacgct gtctgaaaaa gccaagcctg ccctggaaga tctgcgccag
     1621 gggctgctgc cggttctgga aagctttaaa gtcagtttcc tgtccgcgct ggaagaatat
     1681 accaaaaaac tgaataccca gctgccgggt accggtgcag ctgccctgga acaccatcat
     1741 caccatcact aactcgag
//
