LOCUS       sequence-256433-        2838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Covalently circularized nanodisc NW50 for studying membrane proteins
            and viral entry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Nasr ML, Baptista D, Strauss M, Sun ZJ, Grigoriu S, Huser S, 
            Pluckthun A, Hagn F, Walz T, Hogle JM, Wagner G
  TITLE     Covalently circularized nanodiscs for studying membrane proteins and
            viral entry.
  JOURNAL   Nat Methods. 2017 Jan;14(1):49-52. doi: 10.1038/nmeth.4079. Epub 
            2016 Nov 21.
  PUBMED    27869813
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4079"; journalName: "Nat Methods"; date: "2017-01"; 
            volume: "14"; issue: "1"; pages: "49-52"
COMMENT     Sequence Label: NW50
FEATURES             Location/Qualifiers
     source          1..2838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..33
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..54
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2812..2829
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 catatgggca gctctcatca ccatcaccat cacgaaaacc tgtattttca gggcagtacc
       61 ttctccaaac tgcgcgaaca gctgggtccg gtgacgcaag aattctggga taacctggaa
      121 aaagaaaccg aaggcctgcg tcaggaaatg agtaaagacc tggaagaagt gaaagccaaa
      181 gttcaaccgt atctggatga cttccagaaa aaatggcaag aagaaatgga actgtaccgc
      241 cagaaagtcg aaccgctgcg tgccgaactg caggaaggcg cacgccaaaa actgcatgaa
      301 ctgcaagaaa aactgagccc gctgggtgaa gaaatgcgtg atcgcgcccg tgcacatgtt
      361 gatgctctgc gtacccacct ggccccgtat tctgatgaac tgcgtcagcg tctggccgca
      421 cgtctggaag cgctgaaaga aaacggcggt gcccgtctgg cagaatacca tgctaaagcg
      481 accgaacacc tgtcaacgct gtcggaaaaa gctaaaccgg ccctggaaga tctgcgccag
      541 ggtctgctgc cggttctgga atcatttaaa gtcagcttcc tgtctgccct ggaagaatat
      601 acgaaaaaac tgaacaccca gggcacgccg gttacccagg aattttggga caatctggaa
      661 aaagaaacgg aaggcctgcg ccaggaaatg tcgaaagacc tggaagaagt caaagcaaaa
      721 gtgcagccgt acctggacga ttttcagaaa aaatggcaag aagaaatgga gctgtaccgc
      781 cagaaagttg aaccgctgcg cgcggaactg caggaaggcg cccgtcagaa actgcacgaa
      841 ctgcaggaaa aactgagtcc gctgggcgaa gaaatgcgtg atcgtgcccg cgcacacgtt
      901 gatgccctgc gcacgcacct ggccccgtat tccgatgaac tgcgccagcg cctggcagct
      961 cgtctggaag ccctgaaaga aaatggcggt gcccgcctgg ccgaatatca cgccaaagcc
     1021 accgaacacc tgagtacgct gtccgaaaaa gctaaaccgg cgctggaaga tctgcgccaa
     1081 ggtctgctgc cggtgctgga aagctttaaa gtttcattcc tgtcggcgct ggaagaatac
     1141 acgaaaaaac tgaacacgca gggcaccccg gttacccagg aattctggga caacctggaa
     1201 aaggagacgg aaggcctgcg tcaagaaatg agcaaagatc tggaagaagt taaagccaaa
     1261 gtccagccgt acctggacga ctttcagaaa aaatggcaag aagaaatgga gttataccgc
     1321 cagaaagtgg aaccgctgcg cgctgaactg caggaaggcg cgcgtcagaa actgcatgag
     1381 ttacaggaaa aactgtcacc gctgggagag gaaatgcgcg accgtgcccg tgcacacgtt
     1441 gacgccctgc gcacccacct ggcgccgtat tcggacgaac tgcgtcaacg cctggccgcc
     1501 cgcctggaag ccttaaagga aaatggcggt gcccgtctgg ccgaatatca cgcgaaagcc
     1561 acggaacatc tgagcacgct gtctgaaaaa gccaagcctg ccctggaaga tctgcgccag
     1621 gggctgctgc cggtcctgga aagttttaaa gttagtttcc tgtccgcact ggaagagtat
     1681 acgaaaaaac tgaataccca gggcacgccg gtgacccagg aattttggga taatctggaa
     1741 aaggagaccg aaggcctgcg ccaagaaatg tccaaggatc tggaagaagt gaaagcgaaa
     1801 gtgcagccgt atctggacga tttccagaaa aaatggcaag aagaaatgga gctatatcgt
     1861 caaaaagttg aaccgctgcg tgcggagtta caggaaggcg cccgtcaaaa actgcacgag
     1921 ttacaagaga agctgagccc gctgggcgag gagatgcgcg atcgtgcccg cgcacatgtt
     1981 gacgccctgc gtacgcacct ggcaccgtac agcgacgaac tgcgccaacg cctggccgcc
     2041 cgactggaag ccttaaaaga gaatggcggt gcccgattag ccgaatatca cgcaaaagct
     2101 acggaacatc tgtctaccct gagcgaaaaa gccaagcccg ccctggaaga tctgcgccag
     2161 ggcttattac cggtcctgga aagcttcaaa gttagcttcc tgagcgcgct ggaagaatat
     2221 accaaaaaac tgaataccca aggcaccccg gttacccaag aattttggga caacctggaa
     2281 aaagagacgg agggcctgcg ccaagaaatg agcaaggacc tggaagaagt caaggcgaaa
     2341 gtgcaaccgt acctggacga cttccagaaa aaatggcaag aagaaatgga gctttatcgt
     2401 caaaaagtcg aaccgctgcg cgccgagtta caggaaggcg cgcgtcaaaa actgcacgaa
     2461 ttacaagaga aactgagtcc gctgggagag gagatgcgcg accgcgcccg tgcacacgtg
     2521 gacgcactgc gtacgcatct ggcgccgtac agcgatgagt tacgtcaacg cctggccgcg
     2581 cgcctggaag ctttaaagga gaacggcggt gcccgcctgg cggaatatca cgccaaagct
     2641 acggaacacc tgagcaccct gagcgaaaaa gcgaagccag ccctggaaga tctgcgccag
     2701 ggattattgc cggtcctgga atctttcaaa gtgtcattcc tgagcgccct ggaagaatac
     2761 accaaaaaac tgaacaccca actgccgggt accggtgcag ctgccctgga acaccatcat
     2821 caccatcact aactcgag
//
